RT Journal Article SR Electronic(1) A1 Huang, Xueyong A1 Liu, Licheng A1 Du, Yanhua A1 Ma, Hongxia A1 Mu, Yujiao A1 Pengzhi Wang A1 Ma, Hong A1 Tang, Xiaoyan A1 Wang, Haifeng A1 Kang, Kai A1 Zhang, Shiqiang A1 Wu, Weili A1 Chen, Haomin A1 Liu, Guohua A1 Yang, Yinhui A1 Yongqiangj A1 Jiang A1 Xu, Bianli A1 Chen, WeijunYR 2013 T1 Detection of a novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome in Henan Province, China, using real-time reverse transcription PCR JF Journal of Medical Microbiology, VO 62 IS 7 SP 1060 OP 1064 DO https://doi.org/10.1099/jmm.0.049577-0 PB Microbiology Society, SN 1473-5644, AB A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cross-reactivity towards 13 other viruses that cause similar symptoms. To evaluate the performance of this assay in detecting clinical samples, we analysed 261 serum samples from patients in Henan Province between 2007 and 2010. Of these samples, 91.95 % were bunyavirus positive. Compared with serological assays, the real-time PCR assay was much more sensitive in identifying infected patients 1 to 7 days after the onset of symptoms., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.049577-0