1887

Abstract

This study investigated the species prevalence and antibacterial resistance among enterococci isolated in Kuwait hospitals. They consisted of 415 isolates of (85.3 %), (7.7 %), (4.0 %), (1.2 %), (1.0 %), (0.5 %) and (0.2 %) isolated from urine (36.6 %), blood (10.4 %), wound swabs (11.0 %), stool samples (12.0 %), high vaginal swabs (9.0 %), endocervical swabs (3.0 %) and miscellaneous sources (18.0 %). All of them were susceptible to linezolid. Fifty-two (12.5 %) isolates were ampicillin resistant but none of them produced β-lactamase. They were resistant to erythromycin (63.3 %), tetracycline (60.5 %), ciprofloxacin (40.0 %), chloramphenicol (28.0 %), vancomycin (2.6 %), and teicoplanin (2.6 %). Fourteen, 19 and 20 % of them expressed high-level resistance to gentamicin, kanamycin and streptomycin, respectively. All of the vancomycin-resistant strains carried the phenotype and genotype. There was no evidence of clonal spread of the vancomycin-resistant isolates.

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2003-02-01
2024-12-08
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References

  1. Arthur M, Courvalin P. 1993; Genetics and mechanisms of glycopeptide resistance in enterococci. Antimicrob Agents Chemother 37:1563–1571 [CrossRef]
    [Google Scholar]
  2. Cetinkaya Y, Falk P, Mayhall C. G. 2000; Vancomycin-resistant enterococci. Clin Microbiol Rev 13:686–707 [CrossRef]
    [Google Scholar]
  3. Donskey C. J, Schreiber J. R, Jacobs M. R, Sheker R, Salata R. A, Gordo S, Whalen C. C, Smith F, Rice L. B. 1999; A polyclonal outbreak of predominantly vanB vancomycin-resistant enterococci in North East Ohio. Northeast Ohio vancomycin-resistant enterococcus surveillance program. Clin Infect Dis 29:573–579 [CrossRef]
    [Google Scholar]
  4. Dutka-Malen S, Evers S, Courvalin P. 1995; Detection of glycopeptide resistance and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33:24–27
    [Google Scholar]
  5. Huycke M. M, Sahm D. F, Gilmore M. S. 1998; Multiple-drug resistant enterococci: The nature of the problem and an agenda for the future. Emerg Infect Dis 4:239–249 [CrossRef]
    [Google Scholar]
  6. Ieven M, Vercauteren E, Deschemaeker P, Van Laer F, Goossens H. 1999; Comparison of direct plating and broth enrichment culture for the detection of intestinal colonisation by glycopeptide-resistant enterococci among hospitalized patients. J Clin Microbiol 37:1436–1440
    [Google Scholar]
  7. Jones R. N, Marshall S. A, Pfaller M. A, Wilke W. W, Hollis R. J, Erwin M. E, Edmond M. B, Wenzel R. P. 1997; Nosocomial enterococcal bloodstream infections in the SCOPE program: antimicrobial resistance, species occurrence, molecular testing results and laboratory testing accuracy. SCOPE Hospital Study Group. Diagn Microbiol Infect Dis 29:95–102 [CrossRef]
    [Google Scholar]
  8. Jordens J. Z, Bates J, Griffiths D. J. 1994; Fecal carriage and nosocomial spread of vancomycin-resistant Enterococcus faecium . J Antimicrob Chemother 34:515–528
    [Google Scholar]
  9. Leber A. l, Hindler J. F, Kato E. O, Bruckner D. A, Peques D. A. 2001; Laboratory based surveillance for vancomycin-resistant enterococci: utility of screening stool specimens submitted for Clostridium toxin assay. Infect Control Hosp Epidemiol 22:160–164 [CrossRef]
    [Google Scholar]
  10. Leclercq R, Derlot E, Weber M, Duval J, Courvalin P. 1989; Transferable vancomycin and teicoplanin resistance in Enterococcus faecium . Antimicrob Agents Chemother 33:10–15 [CrossRef]
    [Google Scholar]
  11. Linden P. K, Pasculle A. W, Manez R, Kramer D. J, Fung J. J, Pinna A. D, Kusne S. 1996; Differences in outcomes for patients with bacteremia due to vancomycin-resistant Enterococcus faecium or vancomycin susceptible E. faecium . Clin Infect Dis 22:663–670 [CrossRef]
    [Google Scholar]
  12. Miele A, Bandera M, Goldstein B. P. 1995; Use of primers selective for vancomycin resistance genes to determine van genotypes in enterococci and to study gene organisation in vanA isolates. Antimicrob Agents Chemother 39:1772–1778 [CrossRef]
    [Google Scholar]
  13. Morrison D, Woodford N, Barrett S. P, Sisson P, Cookson B. D. 1999; DNA banding pattern polymorphism in vancomycin-resistant Enterococcus faecium and criteria for defining strains. J Clin Microbiol 37:1084–1091
    [Google Scholar]
  14. National Committee for Clinical Laboratory Standards 2000 Performance standard for disk susceptibility tests, Approved Standard 7th edn. Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  15. Nelson R. R. S, McGregor K. F, Brown A. R, Amyes S. G. B, Young H.-K. 2000; Isolation and characterization of glycopeptide-resistant enterococci from hospitalized patients over a 30-month period. J Clin Microbiol 38:2112–2116
    [Google Scholar]
  16. Perlada D. E, George S. A, Cushion M. T. 1997; Molecular epidemiology and antibiotic susceptibility of enterococci in Cincinnati, Ohio: a prospective citywide survey. J Clin Microbiol 35:2342–2347
    [Google Scholar]
  17. Quale J, Laudman D, Atwood E. 9 other authors 1996; Experience with a hospital-wide outbreak of vancomycin-resistant enterococci. Am J Infect Control 24:372–379 [CrossRef]
    [Google Scholar]
  18. Reinert R. R, Conrad G, Schaeger J. J, Werner G, Witte W, Lutticken R, Klare I. 1999; Survey of antibiotic resistance among enterococci in North Rhine-Wesphalia, Germany. J Clin Microbiol 37:1638–1641
    [Google Scholar]
  19. Schouten M. A, Hoogkamp-Korstanje J. A, Meis J. F, Voss A. 2000; Prevalence of vancomycin-resistant enterococci in Europe. Eur J Clin Microbiol Infect Dis 19:816–822
    [Google Scholar]
  20. Shlaes D. M, Al-Obeid S, Shlaes J. H, Boisivon A, Williamson R. 1989; Inducible, transferable resistance to vancomycin in Enterococcus faecium , D399. J Antimicrob Chemother 23:503–508 [CrossRef]
    [Google Scholar]
  21. Toutouza M, Skandami V, Poujiouka-Bei M, Fakiri H, Karabassi V, Komniou. 2001; Resistance phenotypes in enterococci isolated from clinical specimens during a 3-year period. Clin Microbiol Infect 7:Suppl. 181
    [Google Scholar]
  22. Udo E. E, Dashti A. A. 2000; Detection of genes encoding aminoglycoside modifying enzymes in Staphylococci by polymerase chain reaction and dot blot hybridization. Int J Antimicrob Agents 13:273–279 [CrossRef]
    [Google Scholar]
  23. Werner G, Klare I, Witte W. 1999; Large conjugative vanA plasmids in vancomycin-resistant Enterococcus faecium . J Clin Microbiol 37:2383–2384
    [Google Scholar]
  24. Woodford N, Johnson A. P, George R. C. 1991; Detection of glycopeptide resistance in clinical isolates of Gram-positive bacteria. J Antimicrob Chemother 28:483–486
    [Google Scholar]
  25. Woodford N, Morrison D, Johnson A. P, George R. C. 1993; Antimicrobial resistance amongst enterococci isolated in the United Kingdom: a reference laboratory perspective. J Antimicrob Chemother 32:344–346 [CrossRef]
    [Google Scholar]
  26. Zouain M. G., Araj G. F. 2001; Antimicrobial resistance of Enterococci in Lebanon. Int J Antimicrob Agents 17:209–213 [CrossRef]
    [Google Scholar]
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