A compound produced by Bacillus pumilus (MSH) that inhibits Mucoraceae and Aspergillus species is described. Fungicidal activity was demonstrated by lawn-spotting and by diffusion through 0.45 μm Millipore membranes placed on 5 % sheep-blood agar, nutrient agar, trypticase soy agar and Mueller–Hinton agar, followed by spore inoculation of the bacterium-free underlying agar surface. With either technique, zones of fungal inhibition correlated with the zone of haemolysis produced by B. pumilus (MSH). The active compound inhibited Mucor and Aspergillus spore germination and aborted elongating hyphae, presumably by inducing a cell-wall lesion. Antifungal activity was stable in agar for a minimum of 8 days, resistant to Pronase degradation, and partially inactivated by chloroform exposure and at pH 5.6. Its molecular mass was determined by diffusion through dialysis membrane to be 500–3000 Da. Attempts at further isolation of the compound have proven unsuccessful to date.
BerdyJ.1974; Recent developments of antibiotic research and classification of antibiotics according to chemical structure. Adv Appl Microbiol 18:309–406
KaruseN,
TenyoO,
KobaruS,
KameiH,
MiyakiT,
KonishiM,
OkiT.1990; Pumilucidin, a complex of new antiviral antibiotics. Production, isolation, chemical properties, structure and biological activity. J Antibiot 43:267–280[CrossRef]
MunimbaziC,
BullermanL. B.1998b; Inhibition of aflatoxin production of Aspergillus parasiticus NRRL 2999 by Bacillus pumilus . Mycopathologia 140:163–169
RichardsonM. D,
ShanklandG. S.1995; Rhizopus, Rhizomucor, Absidia and other agents of systemic and subcutaneous zygomycoses. In Manual of Clinical Microbiology , 6th edn. pp 809–846 Edited by
MurrayP. R.,
BaronE. J.,
PhallerM. A.,
TenoverF. C.,
YolkenR. H.
Washington, DC: ASM Press;
Silo-SuhL. A,
LethbridgeB. J,
RaffelS. J,
ClardyJ,
HandelsmanJ.1994; Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW 85. Appl Environ Microbiol 60:2023–2030