1887

Abstract

Two hundred verocytotoxigenic and 216 non-verocytotoxigenic (VTEC and non-VTEC), isolated from a variety of sources were tested for their resistances to 11 antimicrobial agents. The strains included isolates from domestic food animals and both symptomatic and asymptomatic infections in man. A much higher level of resistance was found among the non-VTEC than among the VTEC, regardless of source. The resistant VTEC isolated from animals were predominantly from specimens associated with sick animals. Antibiotic resistance was detected in only four of the 59 (6.8 %) VTEC of human origin, whereas more of the human non-VTEC possessed antibiotic resistance determinants. It was particularly noteworthy that 24/87 (28 %) strains isolated from healthy babies, who had neither contact with antibiotics nor had gastrointestinal symptoms for at least 2 weeks prior to the specimen being taken, were resistant to one or more of the antibiotics tested.

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2003-02-01
2020-01-20
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References

  1. Acheson, D. W. K., Keusch, G. T., Lightowlers, M. & Donohue-Rolfe, A. ( 1990;). Enzyme-linked immunosorbent assay for Shiga toxin and Shiga-like toxin II using P1 glycoprotein from hydatid cysts. J Infect Dis 127, 1145–1150.
    [Google Scholar]
  2. Bettelheim, K. A. ( 1997;). Escherichia coli in the normal flora of humans and animals. In Escherichia coli Mechanisms of Virulence, pp. 85–109. Edited by M. Sussman. Cambridge: Cambridge University Press.
    [Google Scholar]
  3. Bettelheim, K. A. ( 2001;). Enterohaemorrhagic Escherichia coli O157 : H7: a red herring? J Med Microbiol 50, 201–202.
    [Google Scholar]
  4. Bettelheim, K. A. & Thompson, C. J. ( 1987;). New method of serotyping Escherichia coli: implementation and verification. J Clin Microbiol 25, 781–786.
    [Google Scholar]
  5. Bettelheim, K. A., Breadon, A., Faiers, M., O'Farrell, S. M. & Shooter, R. A. ( 1974;a). The origin of ‘O’ serotypes of Escherichia coli in babies after normal delivery. J Hyg 72, 67–70.[CrossRef]
    [Google Scholar]
  6. Bettelheim, K. A., Teoh-Chan, C. H., Chandler, M. E., O'Farrell, S. M., Rahamim, L., Shaw, E. J. & Shooter, R. A. ( 1974;b). Further studies of Escherichia coli in babies after normal delivery. J Hyg 73, 277–285.[CrossRef]
    [Google Scholar]
  7. Bettelheim, K. A., Teoh-Chan, C. H., Chandler, M. E., O'Farrell, S. M., Rahamim, L., Shaw, E. J. & Shooter, R. A. ( 1974;c). Spread of Escherichia coli colonizing new-born babies and their mothers. J Hyg 73, 383–387.[CrossRef]
    [Google Scholar]
  8. Beutin, L., Geier, D., Steinrück, H., Zimmermann, S. & Scheutz, F. ( 1993;). Prevalence and some properies of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J Clin Microbiol 31, 2483–2488.
    [Google Scholar]
  9. Chandler, M. E. & Bettelheim, K. A. ( 1974;). A rapid method of identifying Escherichia coli ‘H’ antigens. Zentbl Bakteriol Parasitenkd Hyg I Abt Orig A 129, 74–79.
    [Google Scholar]
  10. Djordjevic, S. P., Hornitzky, M. A., Bailey, G., Gill, P., Vanselow, B., Walker, K. & Bettelheim, K. A. ( 2001;). Virulence properties and serotypes of Shiga toxin-producing Escherichia coli from healthy Australian slaughter-age sheep. J Clin Microbiol 39, 2017–2021.[CrossRef]
    [Google Scholar]
  11. Dorn, C. R., Scotland, S. M., Smith, H. R., Willshaw, G. A. & Rowe, B. ( 1989;). Properties of verocytotoxin-producing Escherichia coli of human and animal origin belonging to serogroups other than O157 : H7. Epidemiol Infect 103, 83–95.[CrossRef]
    [Google Scholar]
  12. Fagan, P. K., Hornitzky, M. A., Bettelheim, K. A. & Djordjevic, S. P. (1999). Detection of Shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Appl Environ Microbiol 65, 868–872.
  13. Garrett, L. (1995). The Coming Plague. London: Virago Press.
  14. Glynn, M. K., Bopp, C. & Dewitt, W. ( 1998;). Emergence of multidrug-resistant Salmonella enterica serotype typhimurium DT104 infection in the United States. N Engl J Med 338, 1333–1338.[CrossRef]
    [Google Scholar]
  15. Gonzales, E. A. & Blanco, J. ( 1989;). Serotypes and antibiotic resistance of verocytotoxigenic (VTEC) and necrotizing (NTEC) Escherichia coli strains isolated from calves with diarrhoea. FEMS Microbiol Lett 51, 31–36.
    [Google Scholar]
  16. Hornitzky, M. A., Bettelheim, K. A. & Djordjevic, S. P. ( 2000;). The isolation of enterohaemorrhagic Escherichia coli O111 : H- from Australian cattle. Aust Vet J 78, 636–637.[CrossRef]
    [Google Scholar]
  17. Hornitzky, M. A., Bettelheim, K. A. & Djordjevic, S. P. ( 2001;). The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR. FEMS Microbiol Lett 198, 17–22.[CrossRef]
    [Google Scholar]
  18. Johnston, R. E. (1987). In Pig Production Vol. II (Proceeding Number 95), J. D. Stewart Memorial Refresher Course for Veterinarians, pp. 779–788. Sydney, Australia: University of Sydney.
  19. Khan, A., Das, S. C., Ramamurthy, T., Sikdar, A., Khanam, J., Yamasaki, S., Takeda, Y. & Balakrish Nair, G. ( 2002;). Antibiotic resistance, virulence gene, and molecular profiles of Shiga toxin-producing Escherichia coli isolates from diverse sources in Calcutta, India. J Clin Microbiol 40, 2009–2015.[CrossRef]
    [Google Scholar]
  20. Konowalchuk, J., Speirs, J. L. & Stavric, S. (1977). Vero response to a cytotoxin of Escherichia coli. Infect Immun 18, 775–779.
  21. Kudva, I. T., Hatfield, P. G. & Hovde, C. J. ( 1997;). Characterization of Escherichia coli O157 : H7 and other Shiga toxin-producing E. coli serotypes isolated from sheep. J Clin Microbiol 35, 892–899.
    [Google Scholar]
  22. Leung, P. H. M., Yam, W. C., Ng, W. W. S. & Peiris, J. S. M. ( 2001;). The prevalence and characterization of verotoxin-producing Escherichia coli isolated from cattle and pigs in an abattoir in Hong Kong. Epidemiol Infect 126, 173–179.
    [Google Scholar]
  23. Leverstein-van Hall, M. A., Paauw, A., Box, A. T. A., Blok, H. E. M., Verhoef, J. & Fluit, A. C. (2002). Presence of integron-associated resistance in the community is widespread and contributes to multidrug resistance in the hospital. J Clin Microbiol 40, 3038–3040.
  24. Levy, S. & Cruz, J. ( 1999;). Two Americas confront antibiotic resistance. ASM News 65, 132–133.
    [Google Scholar]
  25. Lidin-Janson, G., Falsen, E., Jodal, U., Kaijser, B. & Lincoln, K. ( 1977;). Characteristics of antibiotic resistant Escherichia coli in the rectum of healthy school children. J Med Microbiol 10, 299–308.[CrossRef]
    [Google Scholar]
  26. Paton, A. W., Ratcliff, R. M., Doyle, R. M., Seymour-Murray, J., Davos, D., Lanser, J. A. & Paton, J. C. ( 1996;). Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli. J Clin Microbiol 34, 1622–1627.
    [Google Scholar]
  27. Ramachandran, V., Hornitzky, M. A., Bettelheim, K. A., Walker, M. J. & Djordjevic, S. P. ( 2001;). The common ovine shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2d toxin type. J Clin Microbiol 39, 1932–1937.[CrossRef]
    [Google Scholar]
  28. Richter, H., Klie, H., Timm, M., Gallien, P., Steinruck, H., Perlberg, K. W. & Protz, D. (1997). Verotoxin-producing E. coli (VTEC) in faeces from cattle slaughtered in Germany. Berl Muench Tieraerztl Wochenschr 110, 121–127.
  29. Schroeder, C. M., Zhao, C., DebRoy, C., Torcolini, J., Zhao, S., White, D. G., Wagner, D. D., McDermott, P. F., Walker, R. D. & Meng, J. ( 2002;). Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. Appl Environ Microbiol 68, 576–581.[CrossRef]
    [Google Scholar]
  30. Sotto, A., de Boever, C. M., Fabbro-Peray, P. Gouby, A., Sirot, D. & Jourdan, J. ( 2001;). Risk factors for antibiotic-resistant Escherichia coli isolated from hospitalized patients with urinary tract infections: a prospective study. J Clin Microbiol 39, 438–444.[CrossRef]
    [Google Scholar]
  31. Starr, M., Bennett-Wood, V., Bigham, A. K., de Koning-Ward, T. F., Bordun, A. M., Lightfoot, D., Bettelheim, K. A., Jones, C. L. & Robins-Browne, R. M. ( 1998;). Hemolytic-uremic syndrome following urinary tract infection with enterohemorrhagic Escherichia coli: case report and review. Clin Infect Dis 27, 310–315.[CrossRef]
    [Google Scholar]
  32. Stephan, R. & Kuhn, K. ( 1999;). Prevalence of verotoxin-producing Escherichia coli (VTEC) in bovine coli mastitis and their antibiotic resistance. J Vet Med 46, 423–427.[CrossRef]
    [Google Scholar]
  33. Threlfall, E. J., Ward, L. R. & Rowe, B. ( 1997;). Increasing incidence of resistance to trimethoprim and ciprofloxacin in epidemic Salmonella typhimurium DT104 in England and Wales. Eurosurveillance 2, 81–84.
    [Google Scholar]
  34. Wells, J. G., Shipman, L. D., Green, K. D. & other authors ( 1991;). Isolation of Escherichia coli O157 : H7 and other Shiga-like toxin-producing E. coli from dairy cattle. J Clin Microbiol 29, 985–989.
    [Google Scholar]
  35. White, P. A., McIver, C. J. & Rawlinson W. D. ( 2001;). Integrons and gene cassettes in the Enterobacteriaceae. Antimicrob Agents Chemother 45, 2658–2661.[CrossRef]
    [Google Scholar]
  36. Wieler, L. H., Vieler, E., Erpenstein, C., Schlapp, T., Steinruck, H., Bauerfeind, R., Byomi, A. & Baljer, G. ( 1996;). Shiga toxin-producing Escherichia coli strains from bovines: association of adhesion with carriage of eae and other genes. J Clin Microbiol 34, 2980–2984.
    [Google Scholar]
  37. Willis, C. ( 2000;). Antibiotics in the food chain: their impact on the consumer. Rev Med Microbiol 11, 153–160.[CrossRef]
    [Google Scholar]
  38. Willshaw, G. A., Cheasty, T., Smith, H. R., O'Brien, S. J. & Adak, G. K. ( 2001;). Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995–1998. J Med Microbiol 50, 135–142.
    [Google Scholar]
  39. Wong, C. S., Jelacic, S., Habeeb, R. L., Watkins, S. L. & Tarr, P. I. ( 2000;). The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157 : H7 infections. N Engl J Med 342, 1930–1936.[CrossRef]
    [Google Scholar]
  40. Zhao, S., White, D. G., Ge, B., Ayers, S., Friedman, S., English, L., Wagner, D., Gaines, S. & Meng, J. ( 2001;). Identification and characterization of integron-mediated antibiotic resistance among shiga toxin-producing Escherichia coli isolates. Appl Environ Microbiol 67, 1558–1564.[CrossRef]
    [Google Scholar]
  41. Zimmerhackl, L. B. ( 2000;). E. coli, antibiotics, and the hemolytic-uremic syndrome. N Engl J Med 342, 1990–1991.[CrossRef]
    [Google Scholar]
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