1887

Abstract

Multidrug-resistant has become a global problem. This study characterized amikacin-resistant isolated from eight patients during April 2010–March 2011 in our university hospital and examined the possible mechanisms in three cases in which carbapenem susceptibility changed to carbapenem resistance during treatment of the patients. The gene, which is one of the 16S rRNA methylase genes and is associated with high MICs of aminoglycosides, was positive in all isolates. The gene and IS and IS- were positive in all isolates, but IS- was positive in only three isolates. The CarO outer-membrane protein was lost in one isolate. In the first case, both the susceptible and the resistant isolates were positive for IS- , but the expression of the gene was increased 3.1-fold in the carbapenem-resistant isolate of the pair. In the second case, the carbapenem-resistant strain became positive for IS- , resulting in 21.5-fold increased expression of , compared to the carbapenem-susceptible strain of the pair. In the third case, the carbapenemase genes remained negative despite the carbapenem resistance, but the expression of the gene was increased 4.6-fold after acquisition of carbapenem resistance. Multilocus sequence typing analysis of two isolates showing representative pulsed-field gel electrophoresis patterns demonstrated that both isolates were classified to sequence type 2 (ST2). These results showed that the 16S rRNA methylase-producing , initially susceptible to carbapenem, acquired carbapenem resistance via diverse mechanisms.

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2013-04-01
2019-12-14
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