1887

Abstract

In this prospective study, the fully automated cobas 4800 CT/NG and the cobas TaqMan CT tests were compared for detection in urine and in genital specimens collected with Copan flocked swabs in culture media. A protocol was also established for the highly sensitive detection of in semen specimens using the cobas 4800 CT/NG test. A total of 708 consecutive urogenital samples (293 male urine samples and 356 vaginal, 45 cervical and 14 urethral swabs) obtained from the Bacteriology Department, as well as 100 consecutive semen samples collected from patients attending the Reproduction Biology Department, Bordeaux University Hospital, France, from July to September 2010, were analysed. Positive and negative agreements between the cobas 4800 CT/NG and cobas TaqMan CT tests were 92.7 % [95 % confidence interval (CI), 82.7–97.1 %] and 99.2 % (95 % CI, 98.2–99.7 %), respectively, with an overall agreement of 98.7 % (699/708). The clinical sensitivity of the cobas 4800 CT/NG assay for ranged from 90.9 to 100 % depending on specimen type, with an overall prevalence of 7.2 % (51/708). The clinical specificity ranged from 99.1 to 100 % depending on specimen type. Dilution of 25 µl semen samples in cobas PCR medium proved to be the most sensitive protocol with the lowest inhibition rate. In conclusion, the cobas 4800 CT/NG test was found to be an effective method for detection of in semen, male urine and genital swab samples collected dry or in universal transport medium.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.048652-0
2013-02-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/2/217.html?itemId=/content/journal/jmm/10.1099/jmm.0.048652-0&mimeType=html&fmt=ahah

References

  1. Bébéar C., de Barbeyrac B.. ( 2009;). Genital Chlamydia trachomatis infections. . Clin Microbiol Infect 15:, 4–10. [CrossRef][PubMed]
    [Google Scholar]
  2. Bhalla P., Baveja U. K., Chawla R., Saini S., Khaki P., Bhalla K., Mahajan S., Reddy B. S.. ( 2007;). Simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis by PCR in genitourinary specimens from men and women attending an STD clinic. . J Commun Dis 39:, 1–6.[PubMed]
    [Google Scholar]
  3. Cheng A., Qian Q., Kirby J. E.. ( 2011;). Evaluation of the Abbott RealTime CT/NG assay in comparison to the Roche Cobas Amplicor CT/NG assay. . J Clin Microbiol 49:, 1294–1300. [CrossRef][PubMed]
    [Google Scholar]
  4. De Martino S. J., De Barbeyrac B., Piemont Y., Barthel C., Monteil H., Jaulhac B.. ( 2006;). Detection of Chlamydia trachomatis DNA using MagNA Pure DNA extraction and Cobas Amplicor CT/NG amplification. . Clin Microbiol Infect 12:, 576–579. [CrossRef][PubMed]
    [Google Scholar]
  5. Eley A.. ( 2011;). How to detect Chlamydia trachomatis in males?. J Androl 32:, 15–22. [CrossRef][PubMed]
    [Google Scholar]
  6. Eley A., Pacey A. A.. ( 2011;). The value of testing semen for Chlamydia trachomatis in men of infertile couples. . Int J Androl 34:, 391–401. [CrossRef][PubMed]
    [Google Scholar]
  7. Gaydos C. A., Cartwright C. P., Colaninno P., Welsch J., Holden J., Ho S. Y., Webb E. M., Anderson C., Bertuzis R.. & other authors ( 2010;). Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. . J Clin Microbiol 48:, 3236–3243. [CrossRef][PubMed]
    [Google Scholar]
  8. Gaydos C. A., Farshy C., Barnes M., Quinn N., Agreda P., Rivers C. A., Schwebke J., Papp J.. ( 2012;). Can mailed swab samples be dry-shipped for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis by nucleic acid amplification tests?. Diagn Microbiol Infect Dis 73:, 16–20. [CrossRef][PubMed]
    [Google Scholar]
  9. Gdoura R., Kchaou W., Ammar-Keskes L., Chakroun N., Sellemi A., Znazen A., Rebai T., Hammami A.. ( 2008;). Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples. . J Androl 29:, 198–206. [CrossRef][PubMed]
    [Google Scholar]
  10. Hopkins M. J., Ashton L. J., Alloba F., Alawattegama A., Hart I. J.. ( 2010;). Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine. . Sex Transm Infect 86:, 207–211. [CrossRef][PubMed]
    [Google Scholar]
  11. Kerndt P. R., Ferrero D. V., Aynalem G., Monga D., Wang S., Zhang N., Wong C., Liggins M., Meng Q.. ( 2011;). First report of performance of the Versant CT/GC DNA 1.0 assay (kPCR) for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. . J Clin Microbiol 49:, 1347–1353. [CrossRef][PubMed]
    [Google Scholar]
  12. Manavi K.. ( 2006;). A review on infection with Chlamydia trachomatis. . Best Pract Res Clin Obstet Gynaecol 20:, 941–951. [CrossRef][PubMed]
    [Google Scholar]
  13. Ochsendorf F. R.. ( 2008;). Sexually transmitted infections: impact on male fertility. . Andrologia 40:, 72–75. [CrossRef][PubMed]
    [Google Scholar]
  14. Ochsendorf F. R., Ozdemir K., Rabenau H., Fenner T., Oremek R., Milbradt R., Doerr H. W.. ( 1999;). Chlamydia trachomatis and male infertility: chlamydia-IgA antibodies in seminal plasma are C. trachomatis specific and associated with an inflammatory response. . J Eur Acad Dermatol Venereol 12:, 143–152. [CrossRef][PubMed]
    [Google Scholar]
  15. Pannekoek Y., Westenberg S. M., de Vries J., Repping S., Spanjaard L., Eijk P. P., van der Ende A., Dankert J.. ( 2000;). PCR assessment of Chlamydia trachomatis infection of semen specimens processed for artificial insemination. . J Clin Microbiol 38:, 3763–3767.[PubMed]
    [Google Scholar]
  16. Peeling R., Embree J.. ( 2005;). Screening for sexually transmitted infection pathogens in semen samples. . Can J Infect Dis Med Microbiol 16:, 73–76.[PubMed]
    [Google Scholar]
  17. Peipert J. F.. ( 2003;). Clinical practice. Genital chlamydial infections. . N Engl J Med 349:, 2424–2430. [CrossRef][PubMed]
    [Google Scholar]
  18. Rockett R., Goire N., Limnios A., Turra M., Higgens G., Lambert S. B., Bletchly C., Nissen M. D., Sloots T. P., Whiley D. M.. ( 2010;). Evaluation of the cobas 4800 CT/NG test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae. . Sex Transm Infect 86:, 470–473. [CrossRef][PubMed]
    [Google Scholar]
  19. Semeniuk H., Zentner A., Read R., Church D.. ( 2002;). Evaluation of sequential testing strategies using non-amplified and amplified methods for detection of Chlamydia trachomatis in endocervical and urine specimens from women. . Diagn Microbiol Infect Dis 42:, 43–51. [CrossRef][PubMed]
    [Google Scholar]
  20. Simel D. L., Samsa G. P., Matchar D. B.. ( 1991;). Likelihood ratios with confidence: sample size estimation for diagnostic test studies. . J Clin Epidemiol 44:, 763–770. [CrossRef][PubMed]
    [Google Scholar]
  21. Skidmore S., Horner P., Mallinson H.. ( 2006;). Testing specimens for Chlamydia trachomatis. . Sex Transm Infect 82:, 272–275. [CrossRef][PubMed]
    [Google Scholar]
  22. Taylor S. N., Liesenfeld O., Lillis R. A., Body B. A., Nye M., Williams J., Eisenhut C., Hook E. W. III, Van Der Pol B.. ( 2012;). Evaluation of the Roche cobas® CT/NG test for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in male urine. . Sex Transm Dis 39:, 543–549. [CrossRef][PubMed]
    [Google Scholar]
  23. Van Der Pol B., Liesenfeld O., Williams J. A., Taylor S. N., Lillis R. A., Body B. A., Nye M., Eisenhut C., Hook E. W. III. ( 2012;). Performance of the cobas CT/NG test compared to the Aptima AC2 and Viper CTQ/GCQ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. . J Clin Microbiol 50:, 2244–2249. [CrossRef][PubMed]
    [Google Scholar]
  24. Witkin S. S.. ( 2002;). Immunological aspects of genital chlamydia infections. . Best Pract Res Clin Obstet Gynaecol 16:, 865–874. [CrossRef][PubMed]
    [Google Scholar]
  25. Wolff H., Neubert U., Volkenandt M., Zöchling N., Schlüpen E. M., Bezold G., Meurer M.. ( 1994;). Detection of Chlamydia trachomatis in semen by antibody–enzyme immunoassay compared with polymerase chain reaction, antigen–enzyme immunoassay, and urethral cell culture. . Fertil Steril 62:, 1250–1254.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.048652-0
Loading
/content/journal/jmm/10.1099/jmm.0.048652-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error