1887

Abstract

The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of . Analysis of cytokine messages showed increased expression of IL12, IFN-γ and TNF-α, but not IL4 mRNAs in spleen cells after infection with the strains RH and Beverley. High levels of circulating IL12 and IFN-γ were detected in the serum of mice infected with strain RH, although TNF-α levels remained low. In contrast, the same cytokines were detected at only low levels in the serum of mice infected with the Beverley strain. Administration of antibody against IL12 or IFN-γ significantly delayed time to death of mice infected with strain RH compared to controls. T-Cell-deficient as well as normal mice were equally infected by strain RH, suggesting that T lymphocytes do not contribute to the response. Depletion of natural killer cells from the splenocyte population abolished the production of IFN-γ. Together, our data suggest that the virulent strain RH induces in BALB/c mice a type 1 cytokine pattern with T-cell-independent overproduction of IL12 and IFN-γ that may be involved in the pathogenesis of this micro-organism.

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2003-10-01
2019-12-11
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