1887

Abstract

The purpose of this study was to screen for reduced susceptibility against imipenem and the presence of the New Delhi metallo-β-lactamase-1 (NDM-1) gene in a collection of (, spp. and ) from different surveillance studies between 2003 and 2010 at the International Centre for Diarrhoeal Disease Research, Bangladesh. None of the ( = 1789) and spp. ( = 90) isolated between 2009 and 2010 from stool samples was resistant or had intermediate susceptibility to imipenem. Among 127 extended-spectrum β-lactamase-producing strains isolated during 2003–2009, three isolates (2.4 %) were resistant to imipenem and were positive for . All these NDM-1-producing strains were isolated in 2008 and were resistant to all antibiotics tested except for tigecycline and colistin. All three isolates were positive for group, group ( ) and genes, whilst two isolates were positive for 16S rRNA methylase () and () genes. One isolate was positive for the gene and one for the gene. The gene was located on a conjugative plasmid of ~23–24 MDa. The PFGE patterns of the isolates were different from each other. This study highlights the occurrence of NDM-1-producing organisms in Bangladesh in 2008. The clonal diversity of the isolates and the transferability of plasmids suggest a wider distribution of NDM-1-producing bacteria in Bangladesh.

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2013-01-01
2019-10-17
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References

  1. Castanheira M., Deshpande L. M., Mathai D., Bell J. M., Jones R. N., Mendes R. E.. ( 2011;). Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY Antimicrobial Surveillance Program, 2006–2007. . Antimicrob Agents Chemother 55:, 1274–1278. [CrossRef][PubMed]
    [Google Scholar]
  2. Cattoir V., Poirel L., Rotimi V., Soussy C. J., Nordmann P.. ( 2007;). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. . J Antimicrob Chemother 60:, 394–397. [CrossRef][PubMed]
    [Google Scholar]
  3. CLSI ( 2010;). Performance Standards for Antimicrobial Susceptibility Testing. Approved Standard M100–S20-U, 20th Informational Supplement (June 2010, Update). Wayne, PA: Clinical and Laboratory Standards Institute.
  4. Doi Y., Arakawa Y.. ( 2007;). 16S ribosomal RNA methylation: emerging resistance mechanism against aminoglycosides. . Clin Infect Dis 45:, 88–94. [CrossRef][PubMed]
    [Google Scholar]
  5. Garrec H., Drieux-Rouzet L., Golmard J. L., Jarlier V., Robert J.. ( 2011;). Comparison of nine phenotypic methods for detection of extended-spectrum β-lactamase production by Enterobacteriaceae. . J Clin Microbiol 49:, 1048–1057. [CrossRef][PubMed]
    [Google Scholar]
  6. Gray J. T., Hungerford L. L., Fedorka-Cray P. J., Headrick M. L.. ( 2004;). Extended-spectrum-cephalosporin resistance in Salmonella enterica isolates of animal origin. . Antimicrob Agents Chemother 48:, 3179–3181. [CrossRef][PubMed]
    [Google Scholar]
  7. Guerra B., Soto S. M., Argüelles J. M., Mendoza M. C.. ( 2001;). Multidrug resistance is mediated by large plasmids carrying a class 1 integron in the emergent Salmonella enterica serotype [4,5,12:i:–]. . Antimicrob Agents Chemother 45:, 1305–1308. [CrossRef][PubMed]
    [Google Scholar]
  8. Islam M. A., Talukdar P. K., Hoque A., Huq M., Nabi A., Ahmed D., Talukder K. A., Pietroni M. A., Hays J. P.. & other authors ( 2012;). Emergence of multidrug-resistant NDM-1-producing Gram-negative bacteria in Bangladesh. . Eur J Clin Microbiol Infect Dis 31:, 2593–2600. [CrossRef][PubMed]
    [Google Scholar]
  9. Kumarasamy K. K., Toleman M. A., Walsh T. R., Bagaria J., Butt F., Balakrishnan R., Chaudhary U., Doumith M., Giske C. G.. & other authors ( 2010;). Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. . Lancet Infect Dis 10:, 597–602. [CrossRef][PubMed]
    [Google Scholar]
  10. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M.. ( 1978;). A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. . Plasmid 1:, 417–420. [CrossRef][PubMed]
    [Google Scholar]
  11. Muzaheed, Doi Y., Adams-Haduch J. M., Endimiani A., Sidjabat H. E., Gaddad S. M., Paterson D. L.. ( 2008;). High prevalence of CTX-M-15-producing Klebsiella pneumoniae among inpatients and outpatients with urinary tract infection in Southern India. . J Antimicrob Chemother 61:, 1393–1394. [CrossRef][PubMed]
    [Google Scholar]
  12. Perry J. D., Naqvi S. H., Mirza I. A., Alizai S. A., Hussain A., Ghirardi S., Orenga S., Wilkinson K., Woodford N.. & other authors ( 2011;). Prevalence of faecal carriage of Enterobacteriaceae with NDM-1 carbapenemase at military hospitals in Pakistan, and evaluation of two chromogenic media. . J Antimicrob Chemother 66:, 2288–2294. [CrossRef][PubMed]
    [Google Scholar]
  13. Pitout J. D., Thomson K. S., Hanson N. D., Ehrhardt A. F., Moland E. S., Sanders C. C.. ( 1998;). β-Lactamases responsible for resistance to expanded-spectrum cephalosporins in Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis isolates recovered in South Africa. . Antimicrob Agents Chemother 42:, 1350–1354.[PubMed]
    [Google Scholar]
  14. Poirel L., Dortet L., Bernabeu S., Nordmann P.. ( 2011a;). Genetic features of blaNDM-1-positive Enterobacteriaceae. . Antimicrob Agents Chemother 55:, 5403–5407. [CrossRef][PubMed]
    [Google Scholar]
  15. Poirel L., Walsh T. R., Cuvillier V., Nordmann P.. ( 2011b;). Multiplex PCR for detection of acquired carbapenemase genes. . Diagn Microbiol Infect Dis 70:, 119–123. [CrossRef][PubMed]
    [Google Scholar]
  16. Ribot E. M., Fair M. A., Gautom R., Cameron D. N., Hunter S. B., Swaminathan B., Barrett T. J.. ( 2006;). Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157 : H7, Salmonella, and Shigella for PulseNet. . Foodborne Pathog Dis 3:, 59–67. [CrossRef][PubMed]
    [Google Scholar]
  17. Ryoo N. H., Kim E.-C., Hong S. G., Park Y. J., Lee K., Bae I. K., Song E. H., Jeong S. H.. ( 2005;). Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. . J Antimicrob Chemother 56:, 698–702. [CrossRef][PubMed]
    [Google Scholar]
  18. Solé M., Pitart C., Roca I., Fàbrega A., Salvador P., Muñoz L., Oliveira I., Gascón J., Marco F., Vila J.. ( 2011;). First description of an Escherichia coli strain producing NDM-1 carbapenemase in Spain. . Antimicrob Agents Chemother 55:, 4402–4404. [CrossRef][PubMed]
    [Google Scholar]
  19. Talukder K. A., Islam M. A., Dutta D. K., Hassan F., Safa A., Nair G. B., Sack D. A.. ( 2002;). Phenotypic and genotypic characterization of serologically atypical strains of Shigella flexneri type 4 isolated in Dhaka, Bangladesh. . J Clin Microbiol 40:, 2490–2497. [CrossRef][PubMed]
    [Google Scholar]
  20. Walsh T. R., Weeks J., Livermore D. M., Toleman M. A.. ( 2011;). Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study. . Lancet Infect Dis 11:, 355–362. [CrossRef][PubMed]
    [Google Scholar]
  21. Yong D., Toleman M. A., Giske C. G., Cho H. S., Sundman K., Lee K., Walsh T. R.. ( 2009;). Characterization of a new metallo-β-lactamase gene, blaNDM-1 and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. . Antimicrob Agents Chemother 53:, 5046–5054. [CrossRef][PubMed]
    [Google Scholar]
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