1887

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Volume 62, Issue 3

Performance of culture media for the isolation and identification of from bovine mastitis Free

Abstract

Rapid isolation and identification of pathogens is a major goal of diagnostic microbiology. In order to isolate and identify , a number of authors have used a variety of selective and/or differential culture media. However, to date, there are no reports comparing the efficacy of selective and differential culture media for isolation from bovine mastitis cases using the 16S rRNA () gene sequence as a gold standard test. In the present study, we evaluated the efficacy of four selective and/or differential culture media for the isolation of from milk samples collected from cows suffering from bovine mastitis. Four hundred and forty isolates were obtained using salt–mannitol agar (SMA, Bioxon), Staphylococcus-110 agar (S110, Bioxon), CHROMAgar Staph aureus (CSA, BD-BBL) and sheep’s blood agar (SBA, BD-BBL). All bacterial isolates were identified by their typical colony morphology in the respective media, by secondary tests (for coagulase and β-haemolysis) and by partial 16S rRNA () gene sequencing as a gold standard test. Sensitivity, positive predictive and negative predictive values were higher for SMA (86.96, 52.63 and 95.95 %, respectively) compared with S110 (70.00, 23.73 and 90.91 %, respectively), CSA (69.23, 28.13 and 95.74 %, respectively) and SBA (68.75, 37.93 and 89.58 %, respectively) while specificity values were similar for all media. Data indicated that the use of culture media for isolation combined with determination of coagulase activity and haemolysis as secondary tests improved accuracy of the identification and was in accordance with gene sequence-analysis compared with the use of the culture media alone.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-03-01
2024-04-18
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References

  1. Baird-Parker A. C. 1962; An improved diagnostic and selective medium for isolating coagulase-positive staphylococci. J Appl Bacteriol 25:12–19 [View Article]
     [Google Scholar]
  2. Becker K., Harmsen D., Mellmann A., Meier C., Schumann P., Peters G., von Eiff C. 2004; Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species. J Clin Microbiol 42:4988–4995 [View Article][PubMed]
     [Google Scholar]
  3. Bhutto A. L., Murray R. D., Woldehiwet Z. 2012; California mastitis test scores as indicators of subclinical intra-mammary infections at the end of lactation in dairy cows. Res Vet Sci 92:13–17 [View Article][PubMed]
     [Google Scholar]
  4. Boerlin P., Kuhnert P., Hüssy D., Schaellibaum M. 2003; Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis. J Clin Microbiol 41:767–771 [View Article][PubMed]
     [Google Scholar]
  5. Boyce J. M., Havill N. L. 2008; Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains. J Clin Microbiol 46:350–351 [View Article][PubMed]
     [Google Scholar]
  6. Braem G., De Vliegher S., Verbist B., Heyndrickx M., Leroy F., De Vuyst L. 2012; Culture-independent exploration of the teat apex microbiota of dairy cows reveals a wide bacterial species diversity. Vet Microbiol 157:383–390 [View Article][PubMed]
     [Google Scholar]
  7. Brown J. H. 1919; The use of blood agar for the study of streptococci. Monograph 9. NY: Rockefeller Institute for Medical Research;
  8. Carricajo A., Treny A., Fonsale N., Bes M., Reverdy M. E., Gille Y., Aubert G., Freydiere A. M. 2001; Performance of the chromogenic medium CHROMagar Staph Aureus and the Staphychrom coagulase test in the detection and identification of Staphylococcus aureus in clinical specimens. J Clin Microbiol 39:2581–2583 [View Article][PubMed]
     [Google Scholar]
  9. Ericsson Unnerstad H., Lindberg A., Persson Waller K., Ekman T., Artursson K., Nilsson-Ost M., Bengtsson B. 2009; Microbial aetiology of acute clinical mastitis and agent-specific risk factors. Vet Microbiol 137:90–97 [View Article][PubMed]
     [Google Scholar]
  10. Espinoza G. J. A., Wiggind S., González A. T., Aguilar U. 2004; Economic sustainability at the farm level: a study on family milk production enterprises in México. Tec Pecu Mex 42:55–70
     [Google Scholar]
  11. Gaillot O., Wetsch M., Fortineau N., Berche P. 2000; Evaluation of CHROMagar Staph. aureus, a new chromogenic medium, for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens. J Clin Microbiol 38:1587–1591[PubMed]
     [Google Scholar]
  12. Ghebremedhin B., Layer F., König W., König B. 2008; Genetic classification and distinguishing of Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences. J Clin Microbiol 46:1019–1025 [View Article][PubMed]
     [Google Scholar]
  13. Graveland H., Duim B., van Duijkeren E., Heederik D., Wagenaar J. A. 2011; Livestock-associated methicillin-resistant Staphylococcus aureus in animals and humans. Int J Med Microbiol 301:630–634 [View Article][PubMed]
     [Google Scholar]
  14. Han Z., Lautenbach E., Fishman N., Nachamkin I. 2007; Evaluation of mannitol salt agar, CHROMagar Staph aureus and CHROMagar MRSA for detection of meticillin-resistant Staphylococcus aureus from nasal swab specimens. J Med Microbiol 56:43–46 [View Article][PubMed]
     [Google Scholar]
  15. Hata E. K., Katsuda K., Kobayashi H., Uchida I., Tanaka K., Eguchi M. 2010; Genetic variation among Staphylococcus aureus strains from bovine milk and their relevance to methicillin-resistant isolates from humans. J Clin Microbiol 48:2130–2139 [View Article][PubMed]
     [Google Scholar]
  16. Kateete D. P., Kimani C. N., Katabazi F. A., Okeng A., Okee M. S., Nanteza A., Joloba M. L., Najjuka F. C. 2010; Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Ann Clin Microbiol Antimicrob 9:23 [View Article][PubMed]
     [Google Scholar]
  17. Landis J. R., Koch G. G. 1977; The measurement of observer agreement for categorical data. Biometrics 33:159–174 [View Article][PubMed]
     [Google Scholar]
  18. Lee D. H., Zo Y. G., Kim S. J. 1996; Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism. Appl Environ Microbiol 62:3112–3120[PubMed]
     [Google Scholar]
  19. Martineau F., Picard F. J., Roy P. H., Ouellette M., Bergeron M. G. 1998; Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. J Clin Microbiol 36:618–623[PubMed]
     [Google Scholar]
  20. Martineau F., Picard F. J., Ke D., Paradis S., Roy P. H., Ouellette M., Bergeron M. G. 2001; Development of a PCR assay for identification of staphylococci at genus and species levels. J Clin Microbiol 39:2541–2547 [View Article][PubMed]
     [Google Scholar]
  21. Miller L. G., Diep B. A. 2008; Clinical practice: colonization, fomites, and virulence: rethinking the pathogenesis of community-associated methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis 46:752–760 [View Article][PubMed]
     [Google Scholar]
  22. Nahimana I., Francioli P., Blanc D. S. 2006; Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 12:1168–1174 [View Article][PubMed]
     [Google Scholar]
  23. National Mastitis Council 1999 Laboratory Handbook on Bovine Mastitis, Rev. ed.. Madison, WI: National Mastitis Council;
     [Google Scholar]
  24. Ollis G. W., Rawluk S. A., Schoonderwoerd M., Schipper C. 1995; Detection of Staphylococcus aureus in bulk tank milk using modified Baird–Parker culture media. Can Vet J 36:619–623[PubMed]
     [Google Scholar]
  25. Pantůcek R., Sedlácek I., Petrás P., Koukalová D., Svec P., Stetina V., Vancanneyt M., Chrastinová L., Vokurková J.& other authors ( 2005; Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys. Int J Syst Evol Microbiol 55:1953–1958 [View Article][PubMed]
     [Google Scholar]
  26. Perry J. D., Freydière A. M. 2007; The application of chromogenic media in clinical microbiology. J Appl Microbiol 103:2046–2055 [View Article][PubMed]
     [Google Scholar]
  27. Perry J. D., Rennison C., Butterworth L. A., Hopley A. L. J., Gould F. K. 2003; Evaluation of S. aureus ID, a new chromogenic agar medium for detection of Staphylococcus aureus. J Clin Microbiol 41:5695–5698 [View Article][PubMed]
     [Google Scholar]
  28. Persson Y., Nyman A.-K. J., Grönlund-Andersson U. 2011; Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. Acta Vet Scand 53:36 [View Article][PubMed]
     [Google Scholar]
  29. Pletinckx L. J., De Bleecker Y., Dewulf J., Rasschaert G., Goddeeris B. M., De Man I. 2012; Evaluation of salt concentrations, chromogenic media and anatomical sampling sites for detection of methicillin-resistant Staphylococcus aureus in pigs. Vet Microbiol 154:363–368 [View Article][PubMed]
     [Google Scholar]
  30. Rice D. N. 1997 Using the California mastitis test (CMT) to detect subclinical mastitis. Bulletin G81–556A. Nebguide Publication. University of Nebraska
  31. Ritter V., Kircher S., Sturm K., Warns P., Dick N. 2009; Evaluation of BD BBL CHROMagar Staph aureus medium using AOAC and ISO culture methods. Performance tested method 100503. J AOAC Int 92:1432–1453[PubMed]
     [Google Scholar]
  32. Sakai H., Procop G. W., Kobayashi N., Togawa D., Wilson D. A., Borden L., Krebs V., Bauer T. W. 2004; Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets. J Clin Microbiol 42:5739–5744 [View Article][PubMed]
     [Google Scholar]
  33. Sánchez G. L. G., Solorio J. L. 2004; Description of the bovine farming system in Cotzio and Téjaro, Michoacán, p. 58. Mérida, Yucatán: Facultad de Medicina Veterinaria y Zootecnia. UADY;
  34. Sánchez G. L. G., Solorio J. L., Santos F. J., Castelan O. A. 2004 Determination of the principal components in the family dairy milk system in the central región of the State of Michoacán, México. Abstracts 8th Pan-American Dairy Congress. Pan-American Dairy Federation. Miami Beach, USA. 23–25 June 2004. p. 18
  35. Scaccabarozzi L., Locatelli C., Pisoni G., Manarolla G., Casula A., Bronzo V., Moroni P. 2011; Short communication: epidemiology and genotyping of Candida rugosa strains responsible for persistent intramammary infections in dairy cows. J Dairy Sci 94:4574–4577 [View Article][PubMed]
     [Google Scholar]
  36. Seker E. 2010; Identification of Candida species isolated from bovine mastitis milk and their in vitro hemolytic activity in Western Turkey. Mycopathologia 169:303–308 [View Article]
     [Google Scholar]
  37. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  38. Staley J. T. 2006; The bacterial species dilemma and the genomic-phylogenetic species concept. Philos Trans R Soc Lond B Biol Sci 361:1899–1909 [View Article][PubMed]
     [Google Scholar]
  39. Stutz K., Stephan R., Tasara T. 2011; SpA, ClfA, and FnbA genetic variations lead to Staphaurex test-negative phenotypes in bovine mastitis Staphylococcus aureus isolates. J Clin Microbiol 49:638–646 [View Article][PubMed]
     [Google Scholar]
  40. Takahashi T., Satoh I., Kikuchi N. 1999; Phylogenetic relationships of 38 taxa of the genus Staphylococcus based on 16S rRNA gene sequence analysis. Int J Syst Bacteriol 49:725–728 [View Article][PubMed]
     [Google Scholar]
  41. Wang Y., Wu C. M., Lu L. M., Ren G. W. N., Cao X. Y., Shen J. Z. 2008; Macrolide-lincosamide-resistant phenotypes and genotypes of Staphylococcus aureus isolated from bovine clinical mastitis. Vet Microbiol 130:118–125 [View Article][PubMed]
     [Google Scholar]
  42. Zadoks R. N., Middleton J. R., McDougall S., Katholm J., Schukken Y. H. 2011; Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. J Mammary Gland Biol Neoplasia 16:357–372 [View Article][PubMed]
     [Google Scholar]
  43. Zaragoza C. S., Olivares R. A., Watty A. E., Moctezuma A. L., Tanaca L. V. 2011; Yeasts isolation from bovine mammary glands under different mastitis status in the Mexican High Plateu. Rev Iberoam Micol 28:79–82 [View Article][PubMed]
     [Google Scholar]
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Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis
J. Med. Microbiol. 62, 369 (2013); https://doi.org/10.1099/jmm.0.046284-0
/content/journal/jmm/10.1099/jmm.0.046284-0
/content/journal/jmm/10.1099/jmm.0.046284-0
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