Skip to content
1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 61, Issue 8

Genetic diversity of locus of enterocyte effacement genes of enteropathogenic isolated from Peruvian children No Access

Abstract

The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded and genes among 181 enteropathogenic (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed , , and alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (<0.001); 28.9 % of diarrhoeal and 17.6 % of control samples were typical EPEC (tEPEC). The distribution of alleles (alpha, beta, beta2 and gamma) and alleles (alpha, beta, gamma and a new variant, -N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (<0.05). -alpha was more common among acute episodes (<0.05). typing resulted in five alleles (alpha, beta, gamma and two new sub-alleles, -alpha2 and -alpha3), while -beta and -gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of , , and alleles were found; the most prevalent combination was -beta, -beta, -beta, -beta (34/181 strains), which was more frequent among tEPEC strains (<0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.045443-0
2012-08-01
2025-03-22
Loading full text...

Full text loading...

References

  1. Afset J. E., Bruant G., Brousseau R., Harel J., Anderssen E., Bevanger L., Bergh K. 2006; Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR. J Clin Microbiol 44:3703–3711 [View Article][PubMed]
     [Google Scholar]
  2. Afset J. E., Anderssen E., Bruant G., Harel J., Wieler L., Bergh K. 2008; Phylogenetic backgrounds and virulence profiles of atypical enteropathogenic Escherichia coli strains from a case-control study using multilocus sequence typing and DNA microarray analysis. J Clin Microbiol 46:2280–2290 [View Article][PubMed]
     [Google Scholar]
  3. Bando S. Y., Andrade F. B., Guth B. E., Elias W. P., Moreira-Filho C. A., Pestana de Castro A. F. 2009; Atypical enteropathogenic Escherichia coli genomic background allows the acquisition of non-EPEC virulence factors. FEMS Microbiol Lett 299:22–30 [View Article][PubMed]
     [Google Scholar]
  4. Blanco M., Blanco J. E., Dahbi G., Mora A., Alonso M. P., Varela G., Gadea M. P., Schelotto F., González E. A., Blanco J. 2006; Typing of intimin (eae) genes from enteropathogenic Escherichia coli (EPEC) isolated from children with diarrhoea in Montevideo, Uruguay: identification of two novel intimin variants (μB and ξR/β2B). J Med Microbiol 55:1165–1174 [View Article][PubMed]
     [Google Scholar]
  5. Castillo A., Eguiarte L. E., Souza V. 2005; A genomic population genetics analysis of the pathogenic enterocyte effacement island in Escherichia coli: the search for the unit of selection. Proc Natl Acad Sci U S A 102:1542–1547 [View Article][PubMed]
     [Google Scholar]
  6. Chen H. D., Frankel G. 2005; Enteropathogenic Escherichia coli: unravelling pathogenesis. FEMS Microbiol Rev 29:83–98 [View Article][PubMed]
     [Google Scholar]
  7. China B., Goffaux F., Pirson V., Mainil J. 1999; Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction. FEMS Microbiol Lett 178:177–182 [View Article][PubMed]
     [Google Scholar]
  8. Contreras C. A., Ochoa T. J., Lacher D. W., DebRoy C., Navarro A., Talledo M., Donnenberg M. S., Ecker L., Gil A. I. other authors 2010; Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children. J Med Microbiol 59:25–31 [View Article][PubMed]
     [Google Scholar]
  9. Dean P., Kenny B. 2009; The effector repertoire of enteropathogenic E. coli: ganging up on the host cell. Curr Opin Microbiol 12:101–109 [View Article][PubMed]
     [Google Scholar]
  10. Deng W., Li Y., Vallance B. A., Finlay B. B. 2001; Locus of enterocyte effacement from Citrobacter rodentium: sequence analysis and evidence for horizontal transfer among attaching and effacing pathogens. Infect Immun 69:6323–6335 [View Article][PubMed]
     [Google Scholar]
  11. Deng W., Puente J. L., Gruenheid S., Li Y., Vallance B. A., Vázquez A., Barba J., Ibarra J. A., O’Donnell P. other authors 2004; Dissecting virulence: systematic and functional analyses of a pathogenicity island. Proc Natl Acad Sci U S A 101:3597–3602[PubMed] [CrossRef]
     [Google Scholar]
  12. Donnenberg M. S., Girón J. A., Nataro J. P., Kaper J. B. 1992; A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence. Mol Microbiol 6:3427–3437 [View Article][PubMed]
     [Google Scholar]
  13. Dulguer M. V., Fabbricotti S. H., Bando S. Y., Moreira-Filho C. A., Fagundes-Neto U., Scaletsky I. C. 2003; Atypical enteropathogenic Escherichia coli strains: phenotypic and genetic profiling reveals a strong association between enteroaggregative E. coli heat-stable enterotoxin and diarrhea. J Infect Dis 188:1685–1694 [View Article][PubMed]
     [Google Scholar]
  14. Frankel G., Phillips A. D., Rosenshine I., Dougan G., Kaper J. B., Knutton S. 1998; Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol 30:911–921 [View Article][PubMed]
     [Google Scholar]
  15. Garrido P., Blanco M., Moreno-Paz M., Briones C., Dahbi G., Blanco J., Blanco J., Parro V. 2006; STEC-EPEC oligonucleotide microarray: a new tool for typing genetic variants of the LEE pathogenicity island of human and animal Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains. Clin Chem 52:192–201 [View Article][PubMed]
     [Google Scholar]
  16. Goffaux F., China B., Mainil J. 2001; Organisation and in vitro expression of esp genes of the LEE (locus of enterocyte effacement) of bovine enteropathogenic and enterohemorrhagic Escherichia coli . Vet Microbiol 83:275–286 [View Article][PubMed]
     [Google Scholar]
  17. Guion C. E., Ochoa T. J., Walker C. M., Barletta F., Cleary T. G. 2008; Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR. J Clin Microbiol 46:1752–1757 [View Article][PubMed]
     [Google Scholar]
  18. Lacher D. W., Steinsland H., Whittam T. S. 2006; Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. FEMS Microbiol Lett 261:80–87 [View Article][PubMed]
     [Google Scholar]
  19. Müller D., Benz I., Liebchen A., Gallitz I., Karch H., Schmidt M. A. 2009; Comparative analysis of the locus of enterocyte effacement and its flanking regions. Infect Immun 77:3501–3513 [View Article][PubMed]
     [Google Scholar]
  20. Nataro J. P., Kaper J. B. 1998; Diarrheagenic Escherichia coli . Clin Microbiol Rev 11:142–201[PubMed]
     [Google Scholar]
  21. Nielsen E. M., Andersen M. T. 2003; Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5′ nuclease PCR assay. J Clin Microbiol 41:2884–2893 [View Article][PubMed]
     [Google Scholar]
  22. Ochoa T. J., Barletta F., Contreras C., Mercado E. 2008; New insights into the epidemiology of enteropathogenic Escherichia coli infection. Trans R Soc Trop Med Hyg 102:852–856 [View Article][PubMed]
     [Google Scholar]
  23. Ochoa T. J., Ecker L., Barletta F., Mispireta M. L., Gil A. I., Contreras C., Molina M., Amemiya I., Verastegui H. other authors 2009; Age-related susceptibility to infection with diarrheagenic Escherichia coli among infants from periurban areas in Lima, Peru. Clin Infect Dis 49:1694–1702 [View Article][PubMed]
     [Google Scholar]
  24. Ruuska T., Vesikari T. 1990; Rotavirus disease in Finnish children: use of numerical scores for clinical severity of diarrhoeal episodes. Scand J Infect Dis 22:259–267 [View Article][PubMed]
     [Google Scholar]
  25. Scaletsky I. C., Fabbricotti S. H., Silva S. O., Morais M. B., Fagundes-Neto U. 2002; HEp-2-adherent Escherichia coli strains associated with acute infantile diarrhea, São Paulo, Brazil. Emerg Infect Dis 8:855–858[PubMed] [CrossRef]
     [Google Scholar]
  26. Torres A. G., Zhou X., Kaper J. B. 2005; Adherence of diarrheagenic Escherichia coli strains to epithelial cells. Infect Immun 73:18–29 [View Article][PubMed]
     [Google Scholar]
  27. Trabulsi L. R., Keller R., Tardelli Gomes T. A. 2002; Typical and atypical enteropathogenic Escherichia coli . Emerg Infect Dis 8:508–513[PubMed] [CrossRef]
     [Google Scholar]
  28. Vieira M. A., Andrade J. R., Trabulsi L. R., Rosa A. C., Dias A. M., Ramos S. R., Frankel G., Gomes T. A. 2001; Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E. coli (EPEC) serogroups that carry EAE and lack the EPEC adherence factor and Shiga toxin DNA probe sequences. J Infect Dis 183:762–772 [View Article][PubMed]
     [Google Scholar]
  29. Wong A. R., Pearson J. S., Bright M. D., Munera D., Robinson K. S., Lee S. F., Frankel G., Hartland E. L. 2011; Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements. Mol Microbiol 80:1420–1438 [View Article][PubMed]
     [Google Scholar]
  30. Yuste M., Orden J. A., De La Fuente R., Ruiz-Santa-Quiteria J. A., Cid D., Martínez-Pulgarín S., Domínguez-Bernal G. 2008; Polymerase chain reaction typing of genes of the locus of enterocyte effacement of ruminant attaching and effacing Escherichia coil . Can J Vet Res 72:444–448[PubMed]
     [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.045443-0
Loading
Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children
J. Med. Microbiol. 61, 1114 (2012); https://doi.org/10.1099/jmm.0.045443-0
/content/journal/jmm/10.1099/jmm.0.045443-0
/content/journal/jmm/10.1099/jmm.0.045443-0
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error