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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 61, Issue 8

Genetic diversity of locus of enterocyte effacement genes of enteropathogenic isolated from Peruvian children No Access

Abstract

The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded and genes among 181 enteropathogenic (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed , , and alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (<0.001); 28.9 % of diarrhoeal and 17.6 % of control samples were typical EPEC (tEPEC). The distribution of alleles (alpha, beta, beta2 and gamma) and alleles (alpha, beta, gamma and a new variant, -N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (<0.05). -alpha was more common among acute episodes (<0.05). typing resulted in five alleles (alpha, beta, gamma and two new sub-alleles, -alpha2 and -alpha3), while -beta and -gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of , , and alleles were found; the most prevalent combination was -beta, -beta, -beta, -beta (34/181 strains), which was more frequent among tEPEC strains (<0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.

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© 2012 SGM
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2012-08-01
2025-03-26
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Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children
J. Med. Microbiol. 61, 1114 (2012); https://doi.org/10.1099/jmm.0.045443-0
/content/journal/jmm/10.1099/jmm.0.045443-0
/content/journal/jmm/10.1099/jmm.0.045443-0
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