1887

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Volume 61, Issue 10

Characterization of a multi-component anthrax vaccine designed to target the initial stages of infection as well as toxaemia Free

Abstract

Current vaccine approaches to combat anthrax are effective; however, they target only a single protein [the protective antigen (PA) toxin component] that is produced after spore germination. PA production is subsequently increased during later vegetative cell proliferation. Accordingly, several aspects of the vaccine strategy could be improved. The inclusion of spore-specific antigens with PA could potentially induce protection to initial stages of the disease. Moreover, adding other epitopes to the current vaccine strategy will decrease the likelihood of encountering a strain of (emerging or engineered) that is refractory to the vaccine. Adding recombinant spore-surface antigens (e.g. BclA, ExsFA/BxpB and p5303) to PA has been shown to augment protection afforded by the latter using a challenge model employing immunosuppressed mice challenged with spores derived from the attenuated Sterne strain of . This report demonstrated similar augmentation utilizing guinea pigs or mice challenged with spores of the fully virulent Ames strain or a non-toxigenic but encapsulated ΔAmes strain of , respectively. Additionally, it was shown that immune interference did not occur if optimal amounts of antigen were administered. By administering the toxin and spore-based immunogens simultaneously, a significant adjuvant effect was also observed in some cases. Thus, these data further support the inclusion of recombinant spore antigens in next-generation anthrax vaccine strategies.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-10-01
2024-04-19
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Characterization of a multi-component anthrax vaccine designed to target the initial stages of infection as well as toxaemia
J. Med. Microbiol. 61, 1380 (2012); https://doi.org/10.1099/jmm.0.045393-0
/content/journal/jmm/10.1099/jmm.0.045393-0
/content/journal/jmm/10.1099/jmm.0.045393-0
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