1887

Abstract

Bacteria of the genus are important primary and opportunistic pathogens. Many are zoonotic agents. In this report, phenotypic (API Coryne analysis), genetic ( and 16S rRNA gene sequencing), and physical methods (MS) were used to distinguish the closely related diphtheroid species and , and to definitively diagnose from cephalic implants of rhesus () and cynomolgus () macaques used in cognitive neuroscience research. Throat and cephalic implant cultures yielded 85 isolates from 43 macaques. Identification by API Coryne yielded ( = 74), ( = 2), or most closely related to ( = 3), and commensals and opportunists ( = 6). The two isolates identified as by API Coryne required genetic and MS analysis for accurate characterization as . Of three isolates identified as by 16S rRNA gene sequencing, only one could be confirmed as such by API Coryne, gene sequencing and MS. This study emphasizes the importance of adjunct methods in identification of coryneforms and is the first isolation of from cephalic implants in macaques.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.045377-0
2012-10-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/10/1401.html?itemId=/content/journal/jmm/10.1099/jmm.0.045377-0&mimeType=html&fmt=ahah

References

  1. Adékambi T. , Drancourt M. , Raoult D. . ( 2009; ). The rpoB gene as a tool for clinical microbiologists. . Trends Microbiol 17:, 37–45. [CrossRef] [PubMed]
    [Google Scholar]
  2. Aubel D. , Renaud F. , Freney J. . ( 1997; ). Genomic diversity of several Corynebacterium species identified by amplification of the 16S–23S rRNA gene spacer region. . Int J Syst Bact 47:, 767–772. [CrossRef]
    [Google Scholar]
  3. Bergin I. L. , Chien C.-C. , Marini R. P. , Fox J. G. . ( 2000; ). Isolation and characterization of Corynebacterium ulcerans from cephalic implants in macaques. . Comp Med 50:, 530–535.[PubMed]
    [Google Scholar]
  4. Contzen M. , Sting R. , Blazey B. , Rau J. . ( 2011; ). Corynebacterium ulcerans from diseased wild boars. . Zoonoses Public Health 58:, 479–488. [CrossRef] [PubMed]
    [Google Scholar]
  5. De Zoysa A. , Hawkey P. M. , Engler K. , George R. , Mann G. , Reilly W. , Taylor D. , Efstratiou A. . ( 2005; ). Characterization of toxigenic Corynebacterium ulcerans strains isolated from humans and domestic cats in the United Kingdom. . J Clin Microbiol 43:, 4377–4381. [CrossRef] [PubMed]
    [Google Scholar]
  6. Dewhirst F. E. , Chen T. , Izard J. , Paster B. J. , Tanner A. C. R. , Yu W.-H. , Lakshmanan A. , Wade W. G. . ( 2010; ). The human oral microbiome. . J Bacteriol 192:, 5002–5017. [CrossRef] [PubMed]
    [Google Scholar]
  7. Dorella F. A. , Pacheco L. G. C. , Oliveira S. C. , Miyoshi A. , Azevedo V. . ( 2006; ). Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. . Vet Res 37:, 201–218. [CrossRef] [PubMed]
    [Google Scholar]
  8. Drancourt M. , Bollet C. , Carlioz A. , Martelin R. , Gayral J.-P. , Raoult D. . ( 2000; ). 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. . J Clin Microbiol 38:, 3623–3630.[PubMed]
    [Google Scholar]
  9. Engler K. H. , Glushkevich T. , Mazurova I. K. , George R. C. , Efstratiou A. . ( 1997; ). A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory. . J Clin Microbiol 35:, 495–498.[PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. Fox J. G. , Frost W. W. . ( 1974; ). Corynebacterium ulcerans mastitis in a bonnet macaque (Macaca radiata). . Lab Anim Sci 24:, 820–822.[PubMed]
    [Google Scholar]
  12. Fox J. G. , Shen Z. , Muthupalani S. , Rogers A. R. , Kirchain S. M. , Dewhirst F. E. . ( 2009; ). Chronic hepatitis, hepatic dysplasia, fibrosis, and biliary hyperplasia in hamsters naturally infected with a novel Helicobacter classified in the H. bilis cluster. . J Clin Microbiol 47:, 3673–3681. [CrossRef] [PubMed]
    [Google Scholar]
  13. Frischmann A. , Knoll A. , Hilbert F. , Zasada A. A. , Kämpfer P. , Busse H.-J. . ( 2011; ). Corynebacterium epidermicanis sp. nov., isolated from a dog’s skin. . Int J Syst Evol Microbiol In Press. E-publication November 11, 2011.
    [Google Scholar]
  14. Funke G. , von Graevenitz A. , Clarridge J. E. III , Bernard K. A. . ( 1997; ). Clinical microbiology of coryneform bacteria. . Clin Microbiol Rev 10:, 125–159.[PubMed]
    [Google Scholar]
  15. Hall A. J. , Cassiday P. K. , Bernard K. A. , Bolt F. , Steigerwalt A. G. , Bixler D. , Pawloski L. C. , Whitney A. M. , Iwaki M. . & other authors ( 2010; ). Novel Corynebacterium diphtheriae in domestic cats. . Emerg Infect Dis 16:, 688–691.[PubMed] [CrossRef]
    [Google Scholar]
  16. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  17. Kämpfer P. . ( 1992; ). Differentiation of Corynebacterium spp., Listeria spp., and related organisms by using fluorogenic substrates. . J Clin Microbiol 30:, 1067–1071.[PubMed]
    [Google Scholar]
  18. Katsukawa C. , Kawahara R. , Inoue K. , Ishii A. , Yamagishi H. , Kida K. , Nishino S. , Nagahama S. , Komiya T. . & other authors ( 2009; ). Toxigenic Corynebacterium ulcerans isolated from the domestic dog for the first time in Japan. . Jpn J Infect Dis 62:, 171–172.[PubMed]
    [Google Scholar]
  19. Khamis A. , Raoult D. , La Scola B. . ( 2004; ). rpoB gene sequencing for identification of Corynebacterium species. . J Clin Microbiol 42:, 3925–3931. [CrossRef] [PubMed]
    [Google Scholar]
  20. Khamis A. , Raoult D. , La Scola B. . ( 2005; ). Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium . . J Clin Microbiol 43:, 1934–1936. [CrossRef] [PubMed]
    [Google Scholar]
  21. Konrad R. , Berger A. , Huber I. , Boschert V. , Hormansdorfer S. , Busch U. , Hogardt M. , Schubert S. , Sing A. . ( 2010; ). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectroscopy as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria. . Euro Surveill 15:, 1–5.
    [Google Scholar]
  22. Lartigue M. F. , Monnet X. , Le Flèche A. , Grimont P. A. D. , Benet J. J. , Durrbach A. , Fabre M. , Nordmann P. . ( 2005; ). Corynebacterium ulcerans in an immunocompromised patient with diphtheria and her dog. . J Clin Microbiol 43:, 999–1001. [CrossRef] [PubMed]
    [Google Scholar]
  23. Lowe C. F. , Bernard K. A. , Romney M. G. . ( 2011; ). Cutaneous diphtheria in the urban poor population of Vancouver, British Columbia, Canada: a 10-year review. . J Clin Microbiol 49:, 2664–2666. [CrossRef] [PubMed]
    [Google Scholar]
  24. Mattos-Guaraldi A. L. , Sampaio J. L. M. , Santos C. S. , Pimenta F. P. , Pereira G. A. , Pacheco L. G. C. , Miyoshi A. , Azevedo V. , Moreira L. O. . & other authors ( 2008; ). First detection of Corynebacterium ulcerans producing a diphtheria-like toxin in a case of human with pulmonary infection in the Rio de Janeiro metropolitan area, Brazil. . Mem Inst Oswaldo Cruz 103:, 396–400. [CrossRef] [PubMed]
    [Google Scholar]
  25. Osanai T. , Miyoshi I. , Hiramune T. , Kasai N. . ( 1994; ). Spontaneous urinary calculus in young LEW rats caused by Corynebacterium renale . . J Urol 152:, 1002–1004.[PubMed]
    [Google Scholar]
  26. Pacheco L. G. C. , Pena R. R. , Castro T. L. P. , Dorella F. A. , Bahia R. C. , Carminati R. , Frota M. N. L. , Oliveira S. C. , Meyer R. . & other authors ( 2007; ). Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. . J Med Microbiol 56:, 480–486. [CrossRef] [PubMed]
    [Google Scholar]
  27. Paster B. J. , Dewhirst F. E. . ( 1988; ). Phylogeny of campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides urealyticus by 16S ribosomal ribonucleic acid sequencing. . Int J Syst Bacteriol 38:, 56–62. [CrossRef]
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Schuhegger R. , Lindermayer M. , Kugler R. , Heesemann J. , Busch U. , Sing A. . ( 2008; ). Detection of toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans strains by a novel real-time PCR. . J Clin Microbiol 46:, 2822–2823. [CrossRef] [PubMed]
    [Google Scholar]
  30. Schuhegger R. , Schoerner C. , Dlugaiczyk J. , Lichtenfeld I. , Trouillier A. , Zeller-Peronnet V. , Busch U. , Berger A. , Kugler R. . & other authors ( 2009; ). Pigs as source for toxigenic Corynebacterium ulcerans . . Emerg Infect Dis 15:, 1314–1315. [CrossRef] [PubMed]
    [Google Scholar]
  31. Selim S. A. . ( 2001; ). Review of oedematous skin disease of buffalo in Egypt. . J Vet Med B 48:, 241–258. [CrossRef]
    [Google Scholar]
  32. Stevens E. L. , Twenhafel N. A. , MacLarty A. M. , Kreiselmeier N. . ( 2007; ). Corynebacterial necrohemorrhagic cystitis in two female macaques. . J Am Assoc Lab Anim Sci 46:, 65–69.[PubMed]
    [Google Scholar]
  33. Takahashi T. , Tsuji M. , Kikuchi N. , Ishihara C. , Osanai T. , Kasai N. , Yanagawa R. , Hiramune T. . ( 1995; ). Assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16S rRNA genes of corynebacteria. . J Vet Med Sci 57:, 515–517. [CrossRef] [PubMed]
    [Google Scholar]
  34. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wagner J. , Ignatius R. , Voss S. , Höpfner V. , Ehlers S. , Funke G. , Weber U. , Hahn H. . ( 2001; ). Infection of the skin caused by Corynebacterium ulcerans and mimicking classical cutaneous diphtheria. . Clin Infect Dis 33:, 1598–1600. [CrossRef] [PubMed]
    [Google Scholar]
  36. Wagner K. S. , White J. M. , Crowcroft N. S. , De Martin S. , Mann G. , Efstratiou A. . ( 2010; ). Diphtheria in the United Kingdom, 1986–2008: the increasing role of Corynebacterium ulcerans . . Epidemiol Infect 138:, 1519–1530. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.045377-0
Loading
/content/journal/jmm/10.1099/jmm.0.045377-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error