1887

Abstract

is a multi-resistant opportunistic nosocomial pathogen responsible for several outbreaks worldwide. It can cause several infections at various sites of the body. One of the main infections caused by this bacterium is ventilator-associated pneumonia in patients in intensive care units. Treating these infections is becoming difficult because of the high resistance to antimicrobial agents. This study compared the expression of the chromosomally encoded gene in isolates having IS, IS and no insertion upstream of the gene in clinical isolates. It showed that the expression of was six times greater when IS was present upstream of the gene in comparison with the constitutively expressed gene with no insertion present upstream. The study indicated that IS has better promoters than IS and this is responsible for the overexpression of the gene as they share considerable homology to the well-established promoters. The −10 box of IS formed a fusion promoter with the −35 box of the gene causing the gene to be significantly overexpressed. The ability to upregulate the expression of with the assistance of different insertion elements such as IS and IS has become an important factor in resistance to cephalosporins.

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2012-08-01
2019-10-16
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