1887

Abstract

Virulence associated with fluconazole (FL) resistance in is a global problem and has not been well characterized at the proteome level. In this study, a stable FL-resistant (MIC >256 µg ml) strain of was generated on agar containing FL. Eight phenotypic mutants were characterized by contour-clamped homogeneous electrophoretic field analysis and two-dimensional PAGE. The secondary derivatives of yielded four distinct genotypes with varying chromosomal profiles. Proteomic analysis performed by tandem mass spectrometry for two of the mutants, CG and CG, demonstrated a total of 25 differentially regulated proteins of which 13 were upregulated and 12 were downregulated or were similar compared with the parental isolate. The mRNA transcript levels of significantly (<0.001) upregulated genes were determined by quantitative RT-PCR analysis, and their physiological relevance in terms of phenotypic expression of virulence attributes was verified by conventional laboratory methodologies. The data showed that the FL resistance (MIC >256 µg ml) of CG was associated with significantly upregulated (<0.001) mRNA transcript levels of several genes – , , , , , and – in addition to a number of other potentially virulent genes expressed differentially at a lower level. The results demonstrated accentuated phenotypic expression of bud formation of yeast and metallothionein production associated with FL resistance in , which may help the fungus to colonize the host.

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2013-02-01
2019-12-11
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