1887

Abstract

colonizes the stomachs of at least half of the world’s human population. The role of the oral cavity in this colonization is not clear and there are, to date, no comprehensive data that clearly demonstrate the isolation of this bacterium from the oral cavity. The aim of this study was to evaluate the prevalence of in the oral cavity of 15 patients who tested positive for . A comprehensive dental examination of all patients was conducted. Samples were taken from supragingival and subgingival plaque, saliva, periapical exudates and tongue swabs. All samples were taken before the application of antibiotics. A total of 163 oral samples were investigated by PCR using two different -specific primer pairs. A PCR inhibition control using a modified plasmid was always included for the most specific primer pair. In addition, a culture technique was used to confirm PCR results. Despite a PCR detection limit of 10 bacteria ml, out of 14 patients, could not be detected in any of the samples taken. In one patient, -positive PCR signals were obtained in two samples using only one primer pair. could not be cultivated from these two PCR-positive samples; therefore, no correlation to oral colonization status could be established. This study challenges the misleading preconception that resides in the human oral cavity and suggests that this bacterium should be considered transient and independent of the oral status. To date, positive PCR results for in the oral cavity have been overestimated and not critically interpreted in literature.

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2012-08-01
2019-10-20
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