1887

Abstract

Seventeen meticillin-resistant coagulase-negative staphylococci (MRCoNS), including ten , four , two and one , and an isolate with various levels of linezolid resistance were isolated from intensive care units in a Chinese hospital. PFGE indicated that the four isolates belonged to a clonal strain, and that nine of the isolates were indistinguishable (clone A1) and the other one was closely related (clone A2). A G2576T mutation was identified in domain V of the 23S rRNA gene in the isolate. Besides the G2576T mutation, a novel C2104T mutation was detected in the nine clone A1 isolates. The gene was detected in all the staphylococci except an isolate, whose 23S rRNA gene contained the G2576T mutation. There was a clonal dissemination of linezolid-resistant MRCoNS in intensive care units of our hospital, and this is the first report, to our knowledge, of linezolid-resistant staphylococci and enterococci in China.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.043729-0
2012-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/11/1568.html?itemId=/content/journal/jmm/10.1099/jmm.0.043729-0&mimeType=html&fmt=ahah

References

  1. An D., Lee M. Y., Jeong T. D., Sung H., Kim M. N., Hong S. B.. ( 2011;). Co-emergence of linezolid-resistant Staphylococcus aureus and Enterococcus faecium in a patient with methicillin-resistant S. aureus pneumonic sepsis. . Diagn Microbiol Infect Dis 69:, 232–233. [CrossRef][PubMed]
    [Google Scholar]
  2. Bonilla H., Huband M. D., Seidel J., Schmidt H., Lescoe M., McCurdy S. P., Lemmon M. M., Brennan L. A., Tait-Kamradt A.. & other authors ( 2010;). Multicity outbreak of linezolid-resistant Staphylococcus epidermidis associated with clonal spread of a cfr-containing strain. . Clin Infect Dis 51:, 796–800. [CrossRef][PubMed]
    [Google Scholar]
  3. Boumghar-Bourtchaï L., Dhalluin A., Malbruny B., Galopin S., Leclercq R.. ( 2009;). Influence of recombination on development of mutational resistance to linezolid in Enterococcus faecalis JH2-2. . Antimicrob Agents Chemother 53:, 4007–4009. [CrossRef][PubMed]
    [Google Scholar]
  4. CLSI ( 2011;). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. CLSI document M100-S21. Wayne, PA: Clinical and Laboratory Standards Institute.
  5. Diekema D. J., Jones R. N.. ( 2001;). Oxazolidinone antibiotics. . Lancet 358:, 1975–1982. [CrossRef][PubMed]
    [Google Scholar]
  6. Gales A. C., Sader H. S., Andrade S. S., Lutz L., Machado A., Barth A. L.. ( 2006;). Emergence of linezolid-resistant Staphylococcus aureus during treatment of pulmonary infection in a patient with cystic fibrosis. . Int J Antimicrob Agents 27:, 300–302. [CrossRef][PubMed]
    [Google Scholar]
  7. Gonzales R. D., Schreckenberger P. C., Graham M. B., Kelkar S., DenBesten K., Quinn J. P.. ( 2001;). Infections due to vancomycin-resistant Enterococcus faecium resistant to linezolid. . Lancet 357:, 1179. [CrossRef][PubMed]
    [Google Scholar]
  8. Howe R. A., Noel A., Bowker K. E., Enne V. I., Walsh T. R., MacGowan A. P.. ( 2002;). Emergence of linezolid (LIN) resistance in Staphylococcus aureus can be associated with loss of erythromycin (ERY) resistance. In Program and Abstracts of the 42nd Interscience Conference on Antimicrobial Agents and Chemotherapy, San Diego, 75. Washington, DC: American Society for Microbiology.
  9. Ikeda-Dantsuji Y., Hanaki H., Sakai F., Tomono K., Takesue Y., Honda J., Nonomiya Y., Suwabe A., Nagura O.. & other authors ( 2011;). Linezolid-resistant Staphylococcus aureus isolated from 2006 through 2008 at six hospitals in Japan. . J Infect Chemother 17:, 45–51. [CrossRef][PubMed]
    [Google Scholar]
  10. Kehrenberg C., Schwarz S.. ( 2006;). Distribution of florfenicol resistance genes fexA and cfr among chloramphenicol-resistant Staphylococcus isolates. . Antimicrob Agents Chemother 50:, 1156–1163. [CrossRef][PubMed]
    [Google Scholar]
  11. Kehrenberg C., Schwarz S., Jacobsen L., Hansen L. H., Vester B.. ( 2005;). A new mechanism for chloramphenicol, florfenicol and clindamycin resistance: methylation of 23S ribosomal RNA at A2503. . Mol Microbiol 57:, 1064–1073. [CrossRef][PubMed]
    [Google Scholar]
  12. Kelly S., Collins J., Maguire M., Gowing C., Flanagan M., Donnelly M., Murphy P. G.. ( 2008;). An outbreak of colonization with linezolid-resistant Staphylococcus epidermidis in an intensive therapy unit. . J Antimicrob Chemother 61:, 901–907. [CrossRef][PubMed]
    [Google Scholar]
  13. Locke J. B., Hilgers M., Shaw K. J.. ( 2009;). Mutations in ribosomal protein L3 are associated with oxazolidinone resistance in staphylococci of clinical origin. . Antimicrob Agents Chemother 53:, 5275–5278. [CrossRef][PubMed]
    [Google Scholar]
  14. Long K. S., Poehlsgaard J., Kehrenberg C., Schwarz S., Vester B.. ( 2006;). The Cfr rRNA methyltransferase confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. . Antimicrob Agents Chemother 50:, 2500–2505. [CrossRef][PubMed]
    [Google Scholar]
  15. Marshall S. H., Donskey C. J., Hutton-Thomas R., Salata R. A., Rice L. B.. ( 2002;). Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis. . Antimicrob Agents Chemother 46:, 3334–3336. [CrossRef][PubMed]
    [Google Scholar]
  16. Meka V. G., Pillai S. K., Sakoulas G., Wennersten C., Venkataraman L., DeGirolami P. C., Eliopoulos G. M., Moellering R. C. Jr, Gold H. S.. ( 2004;). Linezolid resistance in sequential Staphylococcus aureus isolates associated with a T2500A mutation in the 23S rRNA gene and loss of a single copy of rRNA. . J Infect Dis 190:, 311–317. [CrossRef][PubMed]
    [Google Scholar]
  17. Mendes R. E., Deshpande L. M., Farrell D. J., Spanu T., Fadda G., Jones R. N.. ( 2010a;). Assessment of linezolid resistance mechanisms among Staphylococcus epidermidis causing bacteraemia in Rome, Italy. . J Antimicrob Chemother 65:, 2329–2335. [CrossRef][PubMed]
    [Google Scholar]
  18. Mendes R. E., Deshpande L., Rodriguez-Noriega E., Ross J. E., Jones R. N., Morfin-Otero R.. ( 2010b;). First report of Staphylococcal clinical isolates in Mexico with linezolid resistance caused by cfr: evidence of in vivo cfr mobilization. . J Clin Microbiol 48:, 3041–3043. [CrossRef][PubMed]
    [Google Scholar]
  19. Sánchez García M., De la Torre M. A., Morales G., Peláez B., Tolón M. J., Domingo S., Candel F. J., Andrade R., Arribi A.. & other authors ( 2010;). Clinical outbreak of linezolid-resistant Staphylococcus aureus in an intensive care unit. . JAMA 303:, 2260–2264. [CrossRef][PubMed]
    [Google Scholar]
  20. Scheetz M. H., Knechtel S. A., Malczynski M., Postelnick M. J., Qi C.. ( 2008;). Increasing incidence of linezolid-intermediate or -resistant, vancomycin-resistant Enterococcus faecium strains parallels increasing linezolid consumption. . Antimicrob Agents Chemother 52:, 2256–2259. [CrossRef][PubMed]
    [Google Scholar]
  21. Schwarz S., Werckenthin C., Kehrenberg C.. ( 2000;). Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri. . Antimicrob Agents Chemother 44:, 2530–2533. [CrossRef][PubMed]
    [Google Scholar]
  22. Seedat J., Zick G., Klare I., Konstabel C., Weiler N., Sahly H.. ( 2006;). Rapid emergence of resistance to linezolid during linezolid therapy of an Enterococcus faecium infection. . Antimicrob Agents Chemother 50:, 4217–4219. [CrossRef][PubMed]
    [Google Scholar]
  23. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B.. ( 1995;). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. . J Clin Microbiol 33:, 2233–2239.[PubMed]
    [Google Scholar]
  24. Toh S. M., Xiong L., Arias C. A., Villegas M. V., Lolans K., Quinn J., Mankin A. S.. ( 2007;). Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. . Mol Microbiol 64:, 1506–1514. [CrossRef][PubMed]
    [Google Scholar]
  25. Tsiodras S., Gold H. S., Sakoulas G., Eliopoulos G. M., Wennersten C., Venkataraman L., Moellering R. C. Jr, Ferraro M. J.. ( 2001;). Linezolid resistance in a clinical isolate of Staphylococcus aureus. . Lancet 358:, 207–208. [CrossRef][PubMed]
    [Google Scholar]
  26. Wilson P., Andrews J. A., Charlesworth R., Walesby R., Singer M., Farrell D. J., Robbins M.. ( 2003;). Linezolid resistance in clinical isolates of Staphylococcus aureus. . J Antimicrob Chemother 51:, 186–188. [CrossRef][PubMed]
    [Google Scholar]
  27. Wilson D. N., Schluenzen F., Harms J. M., Starosta A. L., Connell S. R., Fucini P.. ( 2008;). The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning. . Proc Natl Acad Sci U S A 105:, 13339–13344. [CrossRef][PubMed]
    [Google Scholar]
  28. Witte W., Cuny C.. ( 2011;). Emergence and spread of cfr-mediated multiresistance in staphylococci: an interdisciplinary challenge. . Future Microbiol 6:, 925–931. [CrossRef][PubMed]
    [Google Scholar]
  29. Wong A., Reddy S. P., Smyth D. S., Aguero-Rosenfeld M. E., Sakoulas G., Robinson D. A.. ( 2010;). Polyphyletic emergence of linezolid-resistant staphylococci in the United States. . Antimicrob Agents Chemother 54:, 742–748. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.043729-0
Loading
/content/journal/jmm/10.1099/jmm.0.043729-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error