1887

Abstract

In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 isolates from diseased food-producing animals in Guangdong province, China. The quinolone resistance-determining regions (QRDRs) of the and genes were analysed for mutations in 55 isolates harbouring only and all isolates harbouring other PMQR genes. Overall, 282 (57.0 %) isolates had at least one PMQR gene. was detected in 215 isolates and predominated the PMQR genes, followed by (63 isolates), (56 isolates), (39 isolates) and (18 isolates). , and were not found in any of the isolates. The rates of resistance to ciprofloxacin, enrofloxacin, levofloxacin and nalidixic acid were 75.2, 81.0, 70.5 and 97.4 %, respectively, among the 495 isolates. Eight types of mutation in were detected in 154 PMQR-positive isolates, and 147 isolates were found to have mutations in . PFGE analysis indicated that the PMQR-positive isolates were genetically diverse. This study demonstrated that the number of mutations in QRDRs of and/or was significantly associated with the MICs of quinolones (<0.01). The rates of resistance to ciprofloxacin, enrofloxacin and nalidixic acid in PMQR-positive isolates were significantly higher than those in PMQR-negative isolates (<0.05). In addition, the prevalence of had significant Spearman correlation coefficients in relation to the MICs of all four tested quinolones (<0.01).

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2012-11-01
2020-08-12
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References

  1. Agersø Y., Aarestrup F. M., Pedersen K., Seyfarth A. M., Struve T., Hasman H. 2012; Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in Danish slaughter pigs and retail meat identified by selective enrichment and association with cephalosporin usage. J Antimicrob Chemother 67:582–588 [CrossRef][PubMed]
    [Google Scholar]
  2. Avsaroglu M. D., Helmuth R., Junker E., Hertwig S., Schroeter A., Akcelik M., Bozoglu F., Guerra B. 2007; Plasmid-mediated quinolone resistance conferred by qnrS1 in Salmonella enterica serovar Virchow isolated from Turkish food of avian origin. J Antimicrob Chemother 60:1146–1150 [CrossRef][PubMed]
    [Google Scholar]
  3. Cattoir V., Poirel L., Nordmann P. 2008; Plasmid-mediated quinolone resistance pump QepA2 in an Escherichia coli isolate from France. Antimicrob Agents Chemother 52:3801–3804 [CrossRef][PubMed]
    [Google Scholar]
  4. Cavaco L. M., Frimodt-Møller N., Hasman H., Guardabassi L., Nielsen L., Aarestrup F. M. 2008; Prevalence of quinolone resistance mechanisms and associations to minimum inhibitory concentrations in quinolone-resistant Escherichia coli isolated from humans and swine in Denmark. Microb Drug Resist 14:163–169 [CrossRef][PubMed]
    [Google Scholar]
  5. Cavaco L. M., Hasman H., Xia S., Aarestrup F. M. 2009; qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin. Antimicrob Agents Chemother 53:603–608 [CrossRef][PubMed]
    [Google Scholar]
  6. CLSI 2008 Performance Standards for Antimicrobial Disk Susceptibility Tests for Bacteria Isolated from Animals, 3rd edn. Approved Standard M31-A3 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  7. Deng Y., Zeng Z., Chen S., He L., Liu Y., Wu C., Chen Z., Yao Q., Hou J. other authors 2011; Dissemination of IncFII plasmids carrying rmtB and qepA in Escherichia coli from pigs, farm workers and the environment. Clin Microbiol Infect 17:1740–1745 [CrossRef][PubMed]
    [Google Scholar]
  8. Everett M. J., Jin Y. F., Ricci V., Piddock L. J. 1996; Contributions of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals. Antimicrob Agents Chemother 40:2380–2386[PubMed]
    [Google Scholar]
  9. Gautom R. K. 1997; Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. J Clin Microbiol 35:2977–2980[PubMed]
    [Google Scholar]
  10. Gay K., Robicsek A., Strahilevitz J., Park C. H., Jacoby G., Barrett T. J., Medalla F., Chiller T. M., Hooper D. C. 2006; Plasmid-mediated quinolone resistance in non-Typhi serotypes of Salmonella enterica.. Clin Infect Dis 43:297–304 [CrossRef][PubMed]
    [Google Scholar]
  11. Hansen L. H., Jensen L. B., Sørensen H. I., Sørensen S. J. 2007; Substrate specificity of the OqxAB multidrug resistance pump in Escherichia coli and selected enteric bacteria. J Antimicrob Chemother 60:145–147 [CrossRef][PubMed]
    [Google Scholar]
  12. Hao H., Yang B., Shi J., Xi M., Wang X., Cui Y., Meng J. 2011; [Drug resistance and related genes of chickenborne Salmonella to quinolone and fluoroquinolones]. Wei Sheng Wu Xue Bao 51:1413–1420[PubMed]
    [Google Scholar]
  13. Hata M., Suzuki M., Matsumoto M., Takahashi M., Sato K., Ibe S., Sakae K. 2005; Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneri 2b. Antimicrob Agents Chemother 49:801–803 [CrossRef][PubMed]
    [Google Scholar]
  14. Heisig P. 1996; Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli.. Antimicrob Agents Chemother 40:879–885[PubMed]
    [Google Scholar]
  15. Hooper D. C. 1999; Mechanisms of fluoroquinolone resistance. Drug Resist Updat 2:38–55 [CrossRef][PubMed]
    [Google Scholar]
  16. Hopkins K. L., Davies R. H., Threlfall E. J. 2005; Mechanisms of quinolone resistance in Escherichia coli and Salmonella: recent developments. Int J Antimicrob Agents 25:358–373 [CrossRef][PubMed]
    [Google Scholar]
  17. Huang S. Y., Dai L., Xia L.-N., Du X.-D., Qi Y.-H., Liu H.-B., Wu C.-M., Shen J.-Z. 2009; Increased prevalence of plasmid-mediated quinolone resistance determinants in chicken Escherichia coli isolates from 2001 to 2007. Foodborne Pathog Dis 6:1203–1209 [CrossRef][PubMed]
    [Google Scholar]
  18. Jacoby G. A., Walsh K. E., Mills D. M., Walker V. J., Oh H., Robicsek A., Hooper D. C. 2006; qnrB, another plasmid-mediated gene for quinolone resistance. Antimicrob Agents Chemother 50:1178–1182 [CrossRef][PubMed]
    [Google Scholar]
  19. Jakobsen L., Kurbasic A., Skjøt-Rasmussen L., Ejrnaes K., Porsbo L. J., Pedersen K., Jensen L. B., Emborg H. D., Agersø Y. other authors 2010; Escherichia coli isolates from broiler chicken meat, broiler chickens, pork, and pigs share phylogroups and antimicrobial resistance with community-dwelling humans and patients with urinary tract infection. Foodborne Pathog Dis 7:537–547 [CrossRef][PubMed]
    [Google Scholar]
  20. Kim H. B., Park C. H., Kim C. J., Kim E.-C., Jacoby G. A., Hooper D. C. 2009; Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period. Antimicrob Agents Chemother 53:639–645 [CrossRef][PubMed]
    [Google Scholar]
  21. Lascols C., Robert J., Cattoir V., Bébéar C., Cavallo J. D., Podglajen I., Ploy M. C., Bonnet R., Soussy C. J., Cambau E. 2007; Type II topoisomerase mutations in clinical isolates of Enterobacter cloacae and other enterobacterial species harbouring the qnrA gene. Int J Antimicrob Agents 29:402–409 [CrossRef][PubMed]
    [Google Scholar]
  22. Lee Y.-J., Cho J.-K., Kim K.-S., Tak R.-B., Kim A.-R., Kim J.-W., Im S.-K., Kim B.-H. 2005; Fluoroquinolone resistance and gyrA and parC mutations of Escherichia coli isolated from chicken. J Microbiol 43:391–397[PubMed]
    [Google Scholar]
  23. Liu B.-T., Wang X.-M., Liao X.-P., Sun J., Zhu H.-Q., Chen X.-Y., Liu Y.-H. 2011; Plasmid-mediated quinolone resistance determinants oqxAB and aac(6′)-Ib-cr and extended-spectrum β-lactamase gene blaCTX-M-24 co-located on the same plasmid in one Escherichia coli strain from China. J Antimicrob Chemother 66:1638–1639 [CrossRef][PubMed]
    [Google Scholar]
  24. Ma J., Zeng Z., Chen Z., Xu X., Wang X., Deng Y., D., Huang L., Zhang Y. other authors 2009; High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6′)-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals. Antimicrob Agents Chemother 53:519–524 [CrossRef][PubMed]
    [Google Scholar]
  25. Mammeri H., Van De Loo M., Poirel L., Martinez-Martinez L., Nordmann P. 2005; Emergence of plasmid-mediated quinolone resistance in Escherichia coli in Europe. Antimicrob Agents Chemother 49:71–76 [CrossRef][PubMed]
    [Google Scholar]
  26. Martínez-Martínez L., Pascual A., Jacoby G. A. 1998; Quinolone resistance from a transferable plasmid. Lancet 351:797–799 [CrossRef][PubMed]
    [Google Scholar]
  27. Nikaido H. 2003; Molecular basis of bacterial outer membrane permeability revisited. Microbiol Mol Biol Rev 67:593–656 [CrossRef][PubMed]
    [Google Scholar]
  28. Park C. H., Robicsek A., Jacoby G. A., Sahm D., Hooper D. C. 2006; Prevalence in the United States of aac(6′)-Ib-cr encoding a ciprofloxacin-modifying enzyme. Antimicrob Agents Chemother 50:3953–3955 [CrossRef][PubMed]
    [Google Scholar]
  29. Pitout J. D., Wei Y., Church D. L., Gregson D. B. 2008; Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6′)-Ib-cr.. J Antimicrob Chemother 61:999–1002 [CrossRef][PubMed]
    [Google Scholar]
  30. Robicsek A., Jacoby G. A., Hooper D. C. 2006a; The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect Dis 6:629–640 [CrossRef][PubMed]
    [Google Scholar]
  31. Robicsek A., Strahilevitz J., Jacoby G. A., Macielag M., Abbanat D., Park C. H., Bush K., Hooper D. C. 2006b; Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. Nat Med 12:83–88 [CrossRef][PubMed]
    [Google Scholar]
  32. Ruiz J. 2003; Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection. J Antimicrob Chemother 51:1109–1117 [CrossRef][PubMed]
    [Google Scholar]
  33. Strahilevitz J., Jacoby G. A., Hooper D. C., Robicsek A. 2009; Plasmid-mediated quinolone resistance: a multifaceted threat. Clin Microbiol Rev 22:664–689 [CrossRef][PubMed]
    [Google Scholar]
  34. Sun Y., Liao X., Wang X., Sun J., Liu B., Zhu H., Zhang Y., Wang Y., Zhang M., Liu Y. 2011; Antimicrobial resistance and virulence factors of Escherichia coli isolated from pork in Guangdong province. Chin J Prev Vet Med 33:32–36
    [Google Scholar]
  35. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239[PubMed]
    [Google Scholar]
  36. Threlfall E. J., Cheasty T., Graham A., Rowe B. 1997; High-level resistance to ciprofloxacin in Escherichia coli.. Lancet 349:403 [CrossRef][PubMed]
    [Google Scholar]
  37. Uchida Y., Mochimaru T., Morokuma Y., Kiyosuke M., Fujise M., Eto F., Harada Y., Kadowaki M., Shimono N., Kang D. 2010; Geographic distribution of fluoroquinolone-resistant Escherichia coli strains in Asia. Int J Antimicrob Agents 35:387–391 [CrossRef][PubMed]
    [Google Scholar]
  38. Wang M., Sahm D. F., Jacoby G. A., Hooper D. C. 2004; Emerging plasmid-mediated quinolone resistance associated with the qnr gene in Klebsiella pneumoniae clinical isolates in the United States. Antimicrob Agents Chemother 48:1295–1299 [CrossRef][PubMed]
    [Google Scholar]
  39. Wang M., Guo Q., Xu X., Wang X., Ye X., Wu S., Hooper D. C., Wang M. 2009; New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis.. Antimicrob Agents Chemother 53:1892–1897 [CrossRef][PubMed]
    [Google Scholar]
  40. Wang Y.-C., Chan J. P.-W., Yeh K.-S., Chang C.-C., Hsuan S.-L., Hsieh Y.-M., Chang Y.-C., Lai T.-C., Lin W.-H., Chen T.-H. 2010; Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates. Vet Microbiol 142:309–312 [CrossRef][PubMed]
    [Google Scholar]
  41. Warburg G., Korem M., Robicsek A., Engelstein D., Moses A. E., Block C., Strahilevitz J. 2009; Changes in aac(6′)-Ib-cr prevalence and fluoroquinolone resistance in nosocomial isolates of Escherichia coli collected from 1991 through 2005. Antimicrob Agents Chemother 53:1268–1270 [CrossRef][PubMed]
    [Google Scholar]
  42. Xia L.-N., Li L., Wu C.-M., Liu Y.-Q., Tao X.-Q., Dai L., Qi Y.-H., Lu L.-M., Shen J.-Z. 2010; A survey of plasmid-mediated fluoroquinolone resistance genes from Escherichia coli isolates and their dissemination in Shandong, China. Foodborne Pathog Dis 7:207–215 [CrossRef][PubMed]
    [Google Scholar]
  43. Xiao Y. H., Wang J., Li Y.MOH National Antimicrobial Resistance Investigation Net 2008; Bacterial resistance surveillance in China: a report from Mohnarin 2004-2005. Eur J Clin Microbiol Infect Dis 27:697–708 [CrossRef][PubMed]
    [Google Scholar]
  44. Xu X., Wu S., Ye X., Liu Y., Shi W., Zhang Y., Wang M. 2007; Prevalence and expression of the plasmid-mediated quinolone resistance determinant qnrA1.. Antimicrob Agents Chemother 51:4105–4110 [CrossRef][PubMed]
    [Google Scholar]
  45. Yamane K., Wachino J., Suzuki S., Kimura K., Shibata N., Kato H., Shibayama K., Konda T., Arakawa Y. 2007; New plasmid-mediated fluoroquinolone efflux pump, QepA, found in an Escherichia coli clinical isolate. Antimicrob Agents Chemother 51:3354–3360 [CrossRef][PubMed]
    [Google Scholar]
  46. Yamane K., Wachino J., Suzuki S., Arakawa Y. 2008; Plasmid-mediated qepA gene among Escherichia coli clinical isolates from Japan. Antimicrob Agents Chemother 52:1564–1566 [CrossRef][PubMed]
    [Google Scholar]
  47. Yang H., Chen S., White D. G., Zhao S., McDermott P., Walker R., Meng J. 2004; Characterization of multiple-antimicrobial-resistant Escherichia coli isolates from diseased chickens and swine in China. J Clin Microbiol 42:3483–3489 [CrossRef][PubMed]
    [Google Scholar]
  48. Yang J., Luo Y., Li J., Ma Y., Hu C., Jin S., Ye L., Cui S. 2010; Characterization of clinical Escherichia coli isolates from China containing transferable quinolone resistance determinants. J Antimicrob Chemother 65:453–459 [CrossRef][PubMed]
    [Google Scholar]
  49. Yue L., Jiang H.-X., Liao X.-P., Liu J.-H., Li S.-J., Chen X.-Y., Chen C.-X., D.-H., Liu Y.-H. 2008; Prevalence of plasmid-mediated quinolone resistance qnr genes in poultry and swine clinical isolates of Escherichia coli.. Vet Microbiol 132:414–420 [CrossRef][PubMed]
    [Google Scholar]
  50. Zhao J., Chen Z., Chen S., Deng Y., Liu Y., Tian W., Huang X., Wu C., Sun Y. other authors 2010a; Prevalence and dissemination of oqxAB in Escherichia coli isolates from animals, farmworkers, and the environment. Antimicrob Agents Chemother 54:4219–4224 [CrossRef][PubMed]
    [Google Scholar]
  51. Zhao X., Xu X., Zhu D., Ye X., Wang M. 2010b; Decreased quinolone susceptibility in high percentage of Enterobacter cloacae clinical isolates caused only by Qnr determinants. Diagn Microbiol Infect Dis 67:110–113 [CrossRef][PubMed]
    [Google Scholar]
  52. Zhou T.-L., Chen X.-J., Zhou M.-M., Zhao Y.-J., Luo X.-H., Bao Q.-Y. 2011; Prevalence of plasmid-mediated quinolone resistance in Escherichia coli isolates in Wenzhou, Southern China, 2002-2008. Jpn J Infect Dis 64:55–57[PubMed]
    [Google Scholar]
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