RT Journal Article SR Electronic(1) A1 Yu, Fangyou A1 Ying, Qunhua A1 Chen, Chun A1 Li, Tingjian A1 Ding, Baixing A1 Liu, Ying A1 Lu, Yuanyuan A1 Qin, Zhiqiang A1 Parsons, Chris A1 Salgado, Cassandra A1 Qu, Di A1 Pan, Jingye A1 Wang, LiangxingYR 2012 T1 Outbreak of pulmonary infection caused by Klebsiella pneumoniae isolates harbouring blaIMP-4 and blaDHA-1 in a neonatal intensive care unit in China JF Journal of Medical Microbiology, VO 61 IS 7 SP 984 OP 989 DO https://doi.org/10.1099/jmm.0.043000-0 PB Microbiology Society, SN 1473-5644, AB Outbreaks caused by Klebsiella pneumoniae producing carbapenemases and other β-lactamases have been reported. Four neonates admitted to a neonatal intensive care unit (NICU) in a Chinese hospital developed respiratory infection while receiving intensive care. In all four cases, multidrug-resistant K. pneumoniae was isolated from multiple respiratory specimens, leading to additional characterization of these organisms and investigation of the local environment in the NICU. Multiple β-lactamase genes, including bla TEM-1, bla IMP-4, bla DHA-1 and bla CTX-M-14, as well as the quinolone resistance gene qnrB4, were harboured by transferable plasmids from all four clinical isolates. Furthermore, PFGE confirmed that three of the four clinical isolates from the patients and three K. pneumoniae isolates collected from the hands of health-care workers and an incubator in the NICU belonged to the same PFGE cluster, indicating that an outbreak due to multidrug-resistant K. pneumoniae carrying bla IMP-4 and bla DHA-1 occurred in this NICU. As far as is known, this is the first report of the co-existence of bla IMP-4 and bla DHA-1 in the same K. pneumoniae isolate. These data suggest that additional precautions are needed to prevent outbreaks of infection caused by multidrug-resistant K. pneumoniae resulting from environmental exposure in NICUs., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.043000-0