1887

Abstract

Recently, much attention has been given to the use of probiotics as an adjuvant for the prevention or treatment of gastrointestinal pathology. The great advantage of therapy with probiotics is that they have few side effects such as selection of resistant bacteria or disturbance of the intestinal microbiota, which occur when antibiotics are used. Adhesion of pathogenic bacteria onto the surface of probiotics instead of onto intestinal receptors could explain part of the probiotic effect. Thus, this study evaluated the adhesion of pathogenic bacteria onto the cell wall of and strains UFMG 905, W303 and BY4741. To understand the mechanism of adhesion of pathogens to yeast, cell-wall mutants of the parental strain of BY4741 were used because of the difficulty of mutating polyploid yeast, as is the case for and . The tests of adhesion showed that, among 11 enteropathogenic bacteria tested, only , Typhimurium and Typhi adhered to the surface of , UFMG 905 and BY4741. The presence of mannose, and to some extent bile salts, inhibited this adhesion, which was not dependent on yeast viability. Among 44 cell-wall mutants of BY4741, five lost the ability to fix the bacteria. Electron microscopy showed that the phenomenon of yeast–bacteria adhesion occurred both and (in the digestive tract of dixenic mice). In conclusion, some pathogenic bacteria were captured on the surface of , UFMG 905 and BY4741, thus preventing their adhesion to specific receptors on the intestinal epithelium and their subsequent invasion of the host.

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2012-09-01
2019-10-17
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References

  1. Boyle R. J. , Robins-Browne R. M. , Tang M. L. K. . ( 2006; ). Probiotic use in clinical practice: what are the risks?. Am J Clin Nutr 83:, 1256–1264.[PubMed]
    [Google Scholar]
  2. Brandão R. L. , Castro I. M. , Bambirra E. A. , Amaral S. C. , Fietto L. G. , Tropia M. J. M. , Neves M. J. , Dos Santos R. G. , Gomes N. C. M. , Nicoli J. R. . ( 1998; ). Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae . . Appl Environ Microbiol 64:, 564–568.[PubMed]
    [Google Scholar]
  3. Buts J. P. , Bernasconi P. , Vaerman J. P. , Dive C. . ( 1990; ). Stimulation of secretory IgA and secretory component of immunoglobulins in small intestine of rats treated with Saccharomyces boulardii . . Dig Dis Sci 35:, 251–256. [CrossRef] [PubMed]
    [Google Scholar]
  4. Castagliuolo I. , Riegler M. F. , Valenick L. , LaMont J. T. , Pothoulakis C. . ( 1999; ). Saccharomyces boulardii protease inhibits the effects of Clostridium difficile toxins A and B in human colonic mucosa. . Infect Immun 67:, 302–307.[PubMed]
    [Google Scholar]
  5. Ciriello C. J. , Lachance M. A. . ( 2001; ). yeastcompare. London, ON, Canada: University of Western Ontario.
  6. COBEA ( 2006; ). Colegio Brasileiro de Experimentação Animal. Legislação e Ética. http://www.cobea.org.br/.
  7. Cusumano C. K. , Hultgren S. J. . ( 2009; ). Bacterial adhesion – a source of alternate antibiotic targets. . IDrugs 12:, 699–705.[PubMed]
    [Google Scholar]
  8. Czerucka D. , Roux I. , Rampal P. . ( 1994; ). Saccharomyces boulardii inhibits secretagogue-mediated adenosine 3′,5′-cyclic monophosphate induction in intestinal cells. . Gastroenterology 106:, 65–72.[PubMed]
    [Google Scholar]
  9. Czerucka D. , Dahan S. , Mograbi B. , Rossi B. , Rampal P. . ( 2000; ). Saccharomyces boulardii preserves the barrier function and modulates the signal transduction pathway induced in enteropathogenic Escherichia coli-infected T84 cells. . Infect Immun 68:, 5998–6004. [CrossRef] [PubMed]
    [Google Scholar]
  10. Dahan S. , Dalmasso G. , Imbert V. , Peyron J.-F. , Rampal P. , Czerucka D. . ( 2003; ). Saccharomyces boulardii interferes with enterohemorrhagic Escherichia coli-induced signaling pathways in T84 cells. . Infect Immun 71:, 766–773. [CrossRef] [PubMed]
    [Google Scholar]
  11. FAO/WHO ( 2002; ). Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, ON, Canada:: Food and Agriculture Organization/World Health Organization;.
    [Google Scholar]
  12. Gedek B. R. . ( 1999; ). Adherence of Escherichia coli serogroup O 157 and the Salmonella Typhimurium mutant DT 104 to the surface of Saccharomyces boulardii . . Mycoses 42:, 261–264. [CrossRef] [PubMed]
    [Google Scholar]
  13. Generoso S. V. , Viana M. , Santos R. , Martins F. S. , Machado J. A. , Arantes R. M. , Nicoli J. R. , Correia M. I. , Cardoso V. N. . ( 2010; ). Saccharomyces cerevisiae strain UFMG 905 protects against bacterial translocation, preserves gut barrier integrity and stimulates the immune system in a murine intestinal obstruction model. . Arch Microbiol 192:, 477–484. [CrossRef] [PubMed]
    [Google Scholar]
  14. Gómez-Zavaglia A. , Kociubinski G. , Pérez P. , Disalvo E. , De Antoni G. . ( 2002; ). Effect of bile on the lipid composition and surface properties of bifidobacteria. . J Appl Microbiol 93:, 794–799. [CrossRef] [PubMed]
    [Google Scholar]
  15. Guglielmetti S. , Tamagnini I. , Minuzzo M. , Arioli S. , Parini C. , Comelli E. , Mora D. . ( 2009; ). Study of the adhesion of Bifidobacterium bifidum MIMBb75 to human intestinal cell lines. . Curr Microbiol 59:, 167–172. [CrossRef] [PubMed]
    [Google Scholar]
  16. Jain S. , Yadav H. , Sinha P. R. , Naito Y. , Marotta F. . ( 2008; ). Dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei has a protective effect against Salmonella enteritidis infection in mice. . Int J Immunopathol Pharmacol 21:, 1021–1029.[PubMed]
    [Google Scholar]
  17. Jain S. , Yadav H. , Sinha P. R. . ( 2009; ). Probiotic dahi containing Lactobacillus casei protects against Salmonella enteritidis infection and modulates immune response in mice. . J Med Food 12:, 576–583. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kline K. A. , Fälker S. , Dahlberg S. , Normark S. , Henriques-Normark B. . ( 2009; ). Bacterial adhesins in host–microbe interactions. . Cell Host Microbe 5:, 580–592. [CrossRef] [PubMed]
    [Google Scholar]
  19. Korhonen T. K. . ( 1979; ). Yeast agglutination of purified enterobacterial pili. . FEMS Microbiol 6:, 421–425. [CrossRef]
    [Google Scholar]
  20. Korhonen T. K. , Leffler H. , Svanborg Edén C. . ( 1981; ). Binding specificity of piliated strains of Escherichia coli and Salmonella Typhimurium to epithelial cells, Saccharomyces cerevisiae cells, and erythrocytes. . Infect Immun 32:, 796–804.[PubMed]
    [Google Scholar]
  21. Kovacs D. J. , Berk T. . ( 2000; ). Recurrent Clostridium difficile-associated diarrhea and colitis treated with Saccharomyces cerevisiae (Baker’s yeast) in combination with antibiotic therapy: a case report. . J Am Board Fam Pract 13:, 138–140.[PubMed] [CrossRef]
    [Google Scholar]
  22. Kumura H. , Tanoue Y. , Tsukahara M. , Tanaka T. , Shimazaki K. . ( 2004; ). Screening of dairy yeast strains for probiotic applications. . J Dairy Sci 87:, 4050–4056. [CrossRef] [PubMed]
    [Google Scholar]
  23. Lachance M.-A. , Bowles J. M. , Starmer W. T. , Barker J. S. F. . ( 1999; ). Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian hibiscus flowers. . Can J Microbiol 45:, 172–177.[PubMed] [CrossRef]
    [Google Scholar]
  24. Martins F. S. , Nardi R. M. D. , Arantes R. M. E. , Rosa C. A. , Neves M. J. , Nicoli J. R. . ( 2005; ). Screening of yeasts as probiotic based on capacities to colonize the gastrointestinal tract and to protect against enteropathogen challenge in mice. . J Gen Appl Microbiol 51:, 83–92. [CrossRef] [PubMed]
    [Google Scholar]
  25. Martins F. S. , Rodrigues A. C. P. , Tiago F. C. P. , Penna F. J. , Rosa C. A. , Arantes R. M. E. , Nardi R. M. D. , Neves M. J. , Nicoli J. R. . ( 2007; ). Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice. . J Med Microbiol 56:, 352–359. [CrossRef] [PubMed]
    [Google Scholar]
  26. Martins F. S. , Veloso L. C. , Arantes R. M. , Nicoli J. R. . ( 2009; ). Effects of yeast probiotic formulation on viability, revival and protection against infection with Salmonella enterica ssp. enterica serovar Typhimurium in mice. . Lett Appl Microbiol 49:, 738–744. [CrossRef] [PubMed]
    [Google Scholar]
  27. Martins F. S. , Dalmasso G. , Arantes R. M. E. , Doye A. , Lemichez E. , Lagadec P. , Imbert V. , Peyron J. F. , Rampal P. . & other authors ( 2010; ). Interaction of Saccharomyces boulardii with Salmonella enterica serovar Typhimurium protects mice and modifies T84 cell response to the infection. . PLoS ONE 5:, e8925. [CrossRef] [PubMed]
    [Google Scholar]
  28. Martins F. S. , Elian S. D. A. , Vieira A. T. , Tiago F. C. P. , Martins A. K. S. , Silva F. C. P. , Souza E. L. S. , Sousa L. P. , Araújo H. R. C. . & other authors ( 2011; ). Oral treatment with Saccharomyces cerevisiae strain UFMG 905 modulates immune responses and interferes with signal pathways involved in the activation of inflammation in a murine model of typhoid fever. . Int J Med Microbiol 301:, 359–364. [CrossRef] [PubMed]
    [Google Scholar]
  29. Pérez-Sotelo L. S. , Talavera-Rojas M. , Monroy-Salazar H. G. , Lagunas-Bernabé S. , Cuarón-Ibargüengoytia J. A. , Jimenez R. M. , Vázquez-Chagoyán J. C. . ( 2005; ). In vitro evaluation of the binding capacity of Saccharomyces cerevisiae Sc47 to adhere to the wall of Salmonella spp. . Rev Latinoam Microbiol 47:, 70–75.[PubMed]
    [Google Scholar]
  30. Pimenta P. F. , De Souza W. . ( 1986; ). Cytochemical localization of acid phosphatase in Leishmania mexicana amazonensis . . J Submicrosc Cytol 18:, 127–132.[PubMed]
    [Google Scholar]
  31. Pleasants J. R. . ( 1974; ). Gnotobiotics. . In Handbook of Laboratory Animal Science, pp. 119–174. Edited by Melby E. C. Jr , Altmann N. H. . . Cleveland, OH:: CRC Press;.
    [Google Scholar]
  32. Qamar A. , Aboudola S. , Warny M. , Michetti P. , Pothoulakis C. , LaMont J. T. , Kelly C. P. . ( 2001; ). Saccharomyces boulardii stimulates intestinal immunoglobulin A immune response to Clostridium difficile toxin A in mice. . Infect Immun 69:, 2762–2765. [CrossRef] [PubMed]
    [Google Scholar]
  33. Rodrigues A. C. P. , Nardi R. M. , Bambirra E. A. , Vieira E. C. , Nicoli J. R. . ( 1996; ). Effect of Saccharomyces boulardii against experimental oral infection with Salmonella typhimurium and Shigella flexneri in conventional and gnotobiotic mice. . J Appl Bacteriol 81:, 251–256. [CrossRef] [PubMed]
    [Google Scholar]
  34. Rodrigues A. C. , Cara D. C. , Fretez S. H. G. G. , Cunha F. Q. , Vieira E. C. , Nicoli J. R. , Vieira L. Q. . ( 2000; ). Saccharomyces boulardii stimulates sIgA production and the phagocytic system of gnotobiotic mice. . J Appl Microbiol 89:, 404–414. [CrossRef] [PubMed]
    [Google Scholar]
  35. Silva L. M. , Carvalho Botelho A. C. , Nacif-Pimenta R. , Martins G. F. , Alves L. C. , Brayner F. A. , Fortes-Dias C. L. , Pimenta P. F. . ( 2008; ). Structural analysis of the venom glands of the armed spider Phoneutria nigriventer (Keyserling, 1891): microanatomy, fine structure and confocal observations. . Toxicon 51:, 693–706. [CrossRef] [PubMed]
    [Google Scholar]
  36. Stratford M. . ( 1993; ). Yeast flocculation: flocculation onset and receptor availability. . Yeast 9:, 85–94. [CrossRef] [PubMed]
    [Google Scholar]
  37. Truusalu K. , Mikelsaar R. H. , Naaber P. , Karki T. , Kullisaar T. , Zilmer M. , Mikelsaar M. . ( 2008; ). Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin. . BMC Microbiol 8:, 132. [CrossRef] [PubMed]
    [Google Scholar]
  38. van der Aa Kühle A. , Skovgaard K. , Jespersen L. . ( 2005; ). In vitro screening of probiotic properties of Saccharomyces cerevisiae var. boulardii and food-borne Saccharomyces cerevisiae strains. . Int J Food Microbiol 101:, 29–39. [CrossRef] [PubMed]
    [Google Scholar]
  39. Vieira L. Q. , dos Santos L. M. , Neumann E. , da Silva A. P. , Moura L. N. , Nicoli J. R. . ( 2008; ). Probiotics protect mice against experimental infections. . J Clin Gastroenterol 42: (Suppl. 3 Pt 2), S168–S169. [CrossRef] [PubMed]
    [Google Scholar]
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