1887

Abstract

The objectives of the present study were to evaluate the production of antagonistic compounds against by strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better producers of such a substance to be tested using a gnotobiotic animal model challenged with one of the more sensitive isolates. A total of 24 isolates from women with and without BV were identified as A higher frequency (<0.05) of this bacterium was observed in women with BV (56.7 %) when compared to healthy women (17.6 %). A total of 86 strains of were obtained from healthy women and women with BV. strains were more frequently present (<0.05) in healthy women (97.5 %) than in women with BV (76.7 %). was the predominating strain in both healthy women and women with BV. , , and were isolated with an intermediate frequency in the two groups. antagonism assays were performed using as indicators 17 reference strains and the strains isolated from women with BV and from healthy women. isolated from healthy women showed the higher antagonistic activity against all the indicator strains when compared with isolates from women with BV. Concerning the indicator strains, found in women with BV was more resistant to the antagonism, particularly when isolates from women with BV were used as producer strains. A high vaginal population level of was obtained by intravaginal inoculation of germ-free mice, and this colonization was accompanied by vaginal histopathological lesions. A tenfold decrease in vaginal population level of and a reduction of histological lesions were observed when the pathogenic challenge was performed in mice previously monoassociated with an strain. Concluding, results of the present study suggest that progression of -associated BV depends in part on a simultaneous presence of populations with a low antagonistic capacity and of a strain with a high resistance to this antagonism. The results could also explain why is frequently found in the vaginal ecosystem of healthy women.

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2012-08-01
2020-01-18
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