1887

Abstract

Xerostomia is a decrease of saliva secretion, which can unbalance the oral microflora, mainly to the benefit of The aim of the present study was to find a plant extract that could create an unfavourable environment for , and would, therefore, be appropriate for use in a dry-mouth daily-care mouthwash. Water extract from the herbaceous plant (Goldenrod) was selected due to its saponin content (plant detergents). Saponin concentrations reached 0.7 and 0.95 mg ml in subsp. and subsp. extracts, respectively. was grown in liquid medium and cells were counted by microscopic examination after 0, 4 and 24 h of incubation. extracts did not inhibit the growth of (four strains), , , , , . When inocula were incubated with extract for 4 and 24 h, we observed a decrease in yeast–hyphal transition. biofilms were then prepared in microtitre plates and treated with plant extracts at 0 h, to estimate biofilm formation, or at 18 h to estimate the effect of the saponin on pre-formed biofilms. Biofilm formation and pre-formed biofilms were both strongly inhibited. In conclusion, the extract was efficient against two key virulence factors of : the yeast–hyphal transition phase and biofilm formation.

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2012-07-01
2019-10-22
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