1887

Abstract

A previously healthy Chinese male returned from working in the Malaysian jungle with a fever. A blood culture grew Gram-negative bacilli that were initially identified as by the VITEK 2 system but were subsequently found to be by partial sequencing of the 16S rRNA gene. The identification of using commercially available automated systems is problematic and clinicians in non-endemic areas should be aware of the possibility of melioidosis in patients with a relevant travel history and blood cultures growing spp.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.041525-0
2012-10-01
2020-08-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/10/1483.html?itemId=/content/journal/jmm/10.1099/jmm.0.041525-0&mimeType=html&fmt=ahah

References

  1. Amornchai P., Chierakul W., Wuthiekanun V., Mahakhunkijcharoen Y., Phetsouvanh R., Currie B. J., Newton P. N., van Vinh Chau N., Wongratanacheewin S. other authors 2007; Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test. J Clin Microbiol 45:3774–3776 [CrossRef][PubMed]
    [Google Scholar]
  2. Cahn A., Koslowsky B., Nir-Paz R., Temper V., Hiller N., Karlinsky A., Gur I., Hidalgo-Grass C., Heyman S. N. other authors 2009; Imported melioidosis, Israel, 2008. Emerg Infect Dis 15:1809–1811[PubMed] [CrossRef]
    [Google Scholar]
  3. Dance D. A., Wuthiekanun V., Naigowit P., White N. J. 1989; Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J Clin Pathol 42:645–648 [CrossRef][PubMed]
    [Google Scholar]
  4. Deepak R. N., Crawley B., Phang E. 2008; Burkholderia pseudomallei identification: a comparison between the API 20NE and VITEK2GN systems. Trans R Soc Trop Med Hyg 102:Suppl. 1S42–S44 [CrossRef][PubMed]
    [Google Scholar]
  5. Giebel R., Worden C., Rust S. M., Kleinheinz G. T., Robbins M., Sandrin T. R. 2010; Microbial fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): applications and challenges. Adv Appl Microbiol 71:149–184 [CrossRef][PubMed]
    [Google Scholar]
  6. Glass M. B., Popovic T. 2005; Preliminary evaluation of the API 20NE and RapID NF plus systems for rapid identification of Burkholderia pseudomallei and B. mallei . J Clin Microbiol 43:479–483 [CrossRef][PubMed]
    [Google Scholar]
  7. Hagen R. M., Frickmann H., Elschner M., Melzer F., Neubauer H., Gauthier Y. P., Racz P., Poppert S. 2011; Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples. Int J Med Microbiol 301:585–590 [CrossRef][PubMed]
    [Google Scholar]
  8. Inglis T. J., Chiang D., Lee G. S., Chor-Kiang L. 1998; Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology 30:62–64 [CrossRef][PubMed]
    [Google Scholar]
  9. Inglis T. J., Merritt A., Chidlow G., Aravena-Roman M., Harnett G. 2005; Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei . J Clin Microbiol 43:2201–2206 [CrossRef][PubMed]
    [Google Scholar]
  10. Kiratisin P., Santanirand P., Chantratita N., Kaewdaeng S. 2007; Accuracy of commercial systems for identification of Burkholderia pseudomallei versus Burkholderia cepacia . Diagn Microbiol Infect Dis 59:277–281 [CrossRef][PubMed]
    [Google Scholar]
  11. Koh T. H., Yong Ng L. S., Foon Ho J. L., Sng L. H., Wang G. C., Tzer Pin Lin R. V. 2003; Automated identification systems and Burkholderia pseudomallei . J Clin Microbiol 41:1809 [CrossRef][PubMed]
    [Google Scholar]
  12. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrant E., Goodfellow M. New York, NY: John Wiley & Sons;
    [Google Scholar]
  13. Lowe P., Engler C., Norton R. 2002; Comparison of automated and nonautomated systems for identification of Burkholderia pseudomallei . J Clin Microbiol 40:4625–4627 [CrossRef][PubMed]
    [Google Scholar]
  14. Lowe P., Haswell H., Lewis K. 2006; Use of various common isolation media to evaluate the new VITEK 2 colorimetric GN Card for identification of Burkholderia pseudomallei . J Clin Microbiol 44:854–856 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.041525-0
Loading

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error