1887

Abstract

The aim of this study was to investigate the molecular characteristics of induced vancomycin resistance in . Autolytic properties and phenotypic characteristics of passage-selected vancomycin-resistant strains were examined. In addition, expression of autolysis-related genes ( and ) was investigated using the RNase protection assay (RPA). The RPA results indicated that only the expression of the gene was significantly upregulated (2.5- to 6-fold increase) in vancomycin-intermediate and vancomycin-resistant strains. The vancomycin-resistant strains exhibited lower expression of murein hydrolase proteins and reduced autolytic activity compared with the parent strain. In addition, a reduced growth rate, cell wall thickening and higher survival rate in the presence of lysostaphin were observed in vancomycin-intermediate and vancomycin-resistant induced strains compared with the parent strain. In conclusion, altered autolytic properties, in particular upregulation of the gene, may contribute to vancomycin resistance in .

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2012-10-01
2019-10-19
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