infection and virulence-associated genes in children with moderate to severe diarrhoea admitted to emergency rooms in northeastern Brazil Free

Abstract

is an important cause of foodborne gastroenteritis. We determined the occurrence of and , using culture-based methods and PCRs targeting virulence-associated genes (VAGs) among children aged ≤14 years who were treated for diarrhoea at emergency rooms in northeastern Brazil. Genomic DNA was extracted directly from stool samples collected from 366 children. A questionnaire was also applied to qualify the clinical conditions presented by each child at the time of admission. and were detected in 16.4 % (60/366) and 1.4 % (5/366) of the diarrhoeal samples, respectively, by PCR, a much higher proportion than that detected by conventional methods. VAGs were detected in the following proportions of -positive samples: , 95 % (57/60); , 86.7 % (52/60); , 98.3 % (59/60); , 80 % (48/60); , 45 % (27/60); , 95 % (57/60); and pVir 0 % (0/60). Particular symptoms, such as blood in faeces, vomiting, fever, and/or abdominal pain, were not associated with detection of nor were they associated with any particular VAG or combination of VAGs (>0.05). and its VAGs were detected in a substantial proportion of the children admitted. Further efforts shall be directed towards elucidating whether these genetic factors or their expressed proteins play a role in pathogenesis.

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2012-04-01
2024-03-28
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