1887

Abstract

is an important cause of foodborne gastroenteritis. We determined the occurrence of and , using culture-based methods and PCRs targeting virulence-associated genes (VAGs) among children aged ≤14 years who were treated for diarrhoea at emergency rooms in northeastern Brazil. Genomic DNA was extracted directly from stool samples collected from 366 children. A questionnaire was also applied to qualify the clinical conditions presented by each child at the time of admission. and were detected in 16.4 % (60/366) and 1.4 % (5/366) of the diarrhoeal samples, respectively, by PCR, a much higher proportion than that detected by conventional methods. VAGs were detected in the following proportions of -positive samples: , 95 % (57/60); , 86.7 % (52/60); , 98.3 % (59/60); , 80 % (48/60); , 45 % (27/60); , 95 % (57/60); and pVir 0 % (0/60). Particular symptoms, such as blood in faeces, vomiting, fever, and/or abdominal pain, were not associated with detection of nor were they associated with any particular VAG or combination of VAGs (>0.05). and its VAGs were detected in a substantial proportion of the children admitted. Further efforts shall be directed towards elucidating whether these genetic factors or their expressed proteins play a role in pathogenesis.

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2012-04-01
2020-01-24
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