1887

Abstract

Variation of gene sequences in group A (GAS) strains was evaluated for its potential use in subtyping the most common serotypes of encountered in the UK. We sequenced a total of 188 strains, including randomly selected invasive GAS and outbreak-related strains. Variability was highest amongst M/ 89 strains, whereas very little variation was observed amongst M/ 1 and M/ 28 GAS strains. Repeat patterns were identified in the collagen structure motif (CSM) of the M/ 89 GAS strains. The sporadic strains were very diverse and encompassed most of the CSM patterns, whereas the outbreak-related strains were mainly clustered into two CSM groups. gene cluster analysis distinguished outbreak strains from two different healthcare settings in the same geographical area. Sequence variations were assessed by the number of pentameric repeats (CAAAA) present at the 5′ region of the gene. The determination of polymorphisms amongst GAS serotype M/ 89 strains could be used as an important epidemiological marker to inform clinicians and outbreak control teams during outbreak investigations.

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2012-05-01
2020-01-22
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