1887

Abstract

This study investigated the genetic environment of genes and associated resistance genes in seven and six extended spectrum β-lactamase (ESBL)-positive isolates. The isolates were recovered from hospitalized patients with respiratory or urinary tract infections at the Military Hospital of Tunis, Tunisia. Twenty-one of the 200 strains exhibited non-susceptibility to third generation cephalosporins and, among these strains, the double-disk synergy test confirmed the ESBL phenotype in 13 isolates. These ESBL producers were co-resistant to chloramphenicol, tetracycline and oflaxacin but remained susceptible to ertapenem (MIC<0.25 mg l). The presence and nature of , , , and genes was determined by PCR and sequencing. Chromosomal localization of the gene was confirmed in all strains, with the exception of isolate M-17991, by Southern-blot analysis performed either on chromosomal or plasmid DNA. M-12012 and M-6019 showed the same PFGE pattern, whereas the remaining CTX-M-15-producing isolates were unrelated. The presence of IS was ascertained in CTX-M-15-producing isolates. A class 1 integron with different gene cassettes (A1, C and B) was found in five and six isolates.

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2012-09-01
2021-10-26
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