1887

Abstract

A novel virulence factor, duodenal ulcer-promoting gene A (), in has been found to be associated with disease in certain populations but not in others. This study analysed a South-east Indian population as part of the debate about the relevance of for the prediction of clinical outcomes. A total of 140 strains isolated from duodenal ulcer (DU) ( = 83) and non-ulcer dyspepsia (NUD) patients ( = 57) were screened by PCR and dot-blot hybridization to determine the presence of the ORFs and . Part of was sequenced to search for the C/T insertion that characterizes and the levels of transcripts were also assessed. The PCR and dot-blot results indicated the presence of and in 37.3 % (31/83) and 12.2 % (7/57) of strains isolated from DU and NUD patients, respectively. Sequencing analysis showed insertion of a C at nt 1386 in the 3′ region of , forming the gene in 35 strains. RT-PCR analysis detected the transcript in 28 of these 35 strains. The expression level of the transcript varied from strain to strain, as shown by real-time PCR. The results demonstrated that analysis based on PCR only for may produce an erroneous interpretation. The prevalence of was significantly greater among strains isolated from patients with DU than from patients with NUD in this population ( = 0.001, odds ratio = 4.26, confidence interval = 1.60–11.74). Based on these findings, can be considered a biomarker for DU patients in India. The reported discrepancies for this putative virulence marker in different populations may be due to the genome plasticity of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.038398-0
2012-09-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/9/1295.html?itemId=/content/journal/jmm/10.1099/jmm.0.038398-0&mimeType=html&fmt=ahah

References

  1. Alm R. A. , Ling L. S. , Moir D. T. , King B. L. , Brown E. D. , Doig P. C. , Smith D. R. , Noonan B. , Guild B. C. . & other authors ( 1999; ). Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori . . Nature 397:, 176–180. [CrossRef] [PubMed]
    [Google Scholar]
  2. Arachchi H. S. , Kalra V. , Lal B. , Bhatia V. , Baba C. S. , Chakravarthy S. , Rohatgi S. , Sarma P. M. , Mishra V. . & other authors ( 2007; ). Prevalence of duodenal ulcer-promoting gene (dupA) of Helicobacter pylori in patients with duodenal ulcer in North Indian population. . Helicobacter 12:, 591–597. [CrossRef] [PubMed]
    [Google Scholar]
  3. Argent R. H. , Burette A. , Miendje Deyi V. Y. , Atherton J. C. . ( 2007; ). The presence of dupA in Helicobacter pylori is not significantly associated with duodenal ulceration in Belgium, South Africa, China, or North America. . Clin Infect Dis 45:, 1204–1206. [CrossRef] [PubMed]
    [Google Scholar]
  4. Atherton J. C. , Cao P. , Peek R. M. Jr , Tummuru M. K. R. , Blaser M. J. , Cover T. L. . ( 1995; ). Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. . J Biol Chem 270:, 17771–17777.[PubMed] [CrossRef]
    [Google Scholar]
  5. Ausubel F. M. , Brent R. , Kingston R. E. , Moore D. D. , Seidman J. G. , Smith J. A. , Struhl K. . ( 1993; ). Current Protocols in Molecular Biology. New York:: Greene Publishing and Wiley-Interscience;.
    [Google Scholar]
  6. Broutet N. , Marais A. , Lamouliatte H. , de Mascarel A. , Samoyeau R. , Salamon R. , Mégraud F. . ( 2001; ). cagA Status and eradication treatment outcome of anti-Helicobacter pylori triple therapies in patients with nonulcer dyspepsia. . J Clin Microbiol 39:, 1319–1322. [CrossRef] [PubMed]
    [Google Scholar]
  7. Brown L. M. . ( 2000; ). Helicobacter pylori: epidemiology and routes of transmission. . Epidemiol Rev 22:, 283–297. [CrossRef] [PubMed]
    [Google Scholar]
  8. Censini S. , Lange C. , Xiang Z. , Crabtree J. E. , Ghiara P. , Borodovsky M. , Rappuoli R. , Covacci A. . ( 1996; ). Cag, pathogenicity island of Helicobacter pylori, encodes type I-specific and diseases associated virulence factors. . Proc Natl Acad Sci USA 93:, 14648–14653. [CrossRef]
    [Google Scholar]
  9. Chattopadhyay S. , Patra R. , Ramamurthy T. , Chowdhury A. , Santra A. , Dhali G. K. , Bhattacharya S. K. , Berg D. E. , Nair G. B. , Mukhopadhyay A. K. . ( 2004; ). Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens. . J Clin Microbiol 42:, 2821–2824. [CrossRef] [PubMed]
    [Google Scholar]
  10. Covacci A. , Censini S. , Bugnoli M. , Petracca R. , Burroni D. , Macchia G. , Massone A. , Papini E. , Xiang Z. . & other authors ( 1993; ). Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. . Proc Natl Acad Sci U S A 90:, 5791–5795. [CrossRef] [PubMed]
    [Google Scholar]
  11. Czinn S. J. . ( 2005; ). Helicobacter pylori infection: detection, investigation, and management. . J Pediatr 146: (Suppl. 3), S21–S26. [CrossRef] [PubMed]
    [Google Scholar]
  12. De Jonge R. , Kuipers E. J. , Langeveld S. C. , Loffeld R. J. , Stoof J. , van Vliet A. H. , Kusters J. G. . ( 2004; ). The Helicobacter pylori plasticity region locus jhp0947–jhp0949 is associated with duodenal ulcer disease and interleukin-12 production in monocyte cells. . FEMS Immunol Med Microbiol 41:, 161–167. [CrossRef] [PubMed]
    [Google Scholar]
  13. Douraghi M. , Mohammadi M. , Oghalaie A. , Abdirad A. , Mohagheghi M. A. , Hosseini M. E. , Zeraati H. , Ghasemi A. , Esmaieli M. , Mohajerani N. . ( 2008; ). dupA as a risk determinant in Helicobacter pylori infection. . J Med Microbiol 57:, 554–562. [CrossRef] [PubMed]
    [Google Scholar]
  14. El-Omar E. M. , Carrington M. , Chow W.-H. , McColl K. E. , Bream J. H. , Young H. A. , Herrera J. , Lissowska J. , Yuan C.-C. . & other authors ( 2000; ). Interleukin-1 polymorphisms associated with increased risk of gastric cancer. . Nature 404:, 398–402. [CrossRef] [PubMed]
    [Google Scholar]
  15. Gomes L. I. , Rocha G. A. , Rocha A. M. , Soares T. F. , Oliveira C. A. , Bittencourt P. F. , Queiroz D. M. . ( 2008; ). Lack of association between Helicobacter pylori infection with dupA-positive strains and gastroduodenal diseases in Brazilian patients. . Int J Med Microbiol 298:, 223–230. [CrossRef] [PubMed]
    [Google Scholar]
  16. Graham D. Y. , Adam E. , Reddy G. T. , Agarwal J. P. , Agarwal R. , Evans D. J. Jr , Malaty H. M. , Evans D. G. . ( 1991; ). Seroepidemiology of Helicobacter pylori infection in India. Comparison of developing and developed countries. . Dig Dis Sci 36:, 1084–1088. [CrossRef] [PubMed]
    [Google Scholar]
  17. Hussein N. R. . ( 2010; ). The association of dupA and Helicobacter pylori-related gastroduodenal diseases. . Eur J Clin Microbiol Infect Dis 29:, 817–821. [CrossRef] [PubMed]
    [Google Scholar]
  18. Hussein N. R. , Mohammadi M. , Talebkhan Y. , Doraghi M. , Letley D. P. , Muhammad M. K. , Argent R. H. , Atherton J. C. . ( 2008; ). Differences in virulence markers between Helicobacter pylori strains from Iraq and those from Iran: potential importance of regional differences in H. pylori-associated disease. . J Clin Microbiol 46:, 1774–1779. [CrossRef] [PubMed]
    [Google Scholar]
  19. Jung S. W. , Sugimoto M. , Shiota S. , Graham D. Y. , Yamaoka Y. . ( 2012; ). The intact dupA cluster is a more reliable Helicobacter pylori virulence marker than dupA alone. . Infect Immun 80:, 381–387. [CrossRef] [PubMed]
    [Google Scholar]
  20. Kersulyte D. , Velapatiño B. , Mukhopadhyay A. K. , Cahuayme L. , Bussalleu A. , Combe J. , Gilman R. H. , Berg D. E. . ( 2003; ). Cluster of type IV secretion genes in Helicobacter pylori’s plasticity zone. . J Bacteriol 185:, 3764–3772. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lu H. , Hsu P.-I. , Graham D. Y. , Yamaoka Y. . ( 2005; ). Duodenal ulcer promoting gene of Helicobacter pylori . . Gastroenterology 128:, 833–848. [CrossRef] [PubMed]
    [Google Scholar]
  22. Machado J. C. , Pharoah P. , Sousa S. , Carvalho R. , Oliveira C. , Figueiredo C. , Amorim A. , Seruca R. , Caldas C. . & other authors ( 2001; ). Interleukin 1B and interleukin 1RN polymorphisms are associated with increased risk of gastric carcinoma. . Gastroenterology 121:, 823–829. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mégraud F. , Lamouliatte H. . ( 1992; ). Helicobacter pylori and duodenal ulcer. Evidence suggesting causation. . Dig Dis Sci 37:, 769–772.[PubMed] [CrossRef]
    [Google Scholar]
  24. Moura S. B. , Costa R. F. A. , Anacleto C. , Rocha G. A. , Rocha A. M. C. , Queiroz D. M. M. . ( 2012; ). Single nucleotide polymorphisms of Helicobacter pylori dupA that lead to premature stop codons. . Helicobacter 17:, 176–180. [CrossRef] [PubMed]
    [Google Scholar]
  25. Mukhopadhyay A. K. , Kersulyte D. , Jeong J. Y. , Datta S. , Ito Y. , Chowdhury A. , Chowdhury S. , Santra A. , Bhattacharya S. K. . & other authors ( 2000; ). Distinctiveness of genotypes of Helicobacter pylori in Calcutta, India. . J Bacteriol 182:, 3219–3227. [CrossRef] [PubMed]
    [Google Scholar]
  26. Nguyen L. T. , Uchida T. , Tsukamoto Y. , Kuroda A. , Okimoto T. , Kodama M. , Murakami K. , Fujioka T. , Moriyama M. . ( 2010; ). Helicobacter pylori dupA gene is not associated with clinical outcomes in the Japanese population. . Clin Microbiol Infect 16:, 1264–1269. [CrossRef] [PubMed]
    [Google Scholar]
  27. Occhialini A. , Marais A. , Alm R. , Garcia F. , Sierra R. , Mégraud F. . ( 2000; ). Distribution of open reading frames of plasticity region of strain J99 in Helicobacter pylori strains isolated from gastric carcinoma and gastritis patients in Costa Rica. . Infect Immun 68:, 6240–6249. [CrossRef] [PubMed]
    [Google Scholar]
  28. Pacheco A. R. , Proença-Módena J. L. , Sales A. I. , Fukuhara Y. , da Silveira W. D. , Pimenta-Módena J. L. , de Oliveira R. B. , Brocchi M. . ( 2008; ). Involvement of the Helicobacter pylori plasticity region and cag pathogenicity island genes in the development of gastroduodenal diseases. . Eur J Clin Microbiol Infect Dis 27:, 1053–1059. [CrossRef] [PubMed]
    [Google Scholar]
  29. Parsonnet J. , Friedman G. D. , Vandersteen D. P. , Chang Y. , Vogelman J. H. , Orentreich N. , Sibley R. K. . ( 1991; ). Helicobacter pylori infection and the risk of gastric carcinoma. . N Engl J Med 325:, 1127–1131.[CrossRef]
    [Google Scholar]
  30. Patra R. , Chattopadhyay S. , De R. , Datta S. , Chowdhury A. , Ramamurthy T. , Nair G. B. , Berg D. E. , Mukhopadhyay A. K. . ( 2011; ). Intact cag pathogenicity island of Helicobacter pylori without disease association in Kolkata, India. . Int J Med Microbiol 301:, 293–302. [CrossRef] [PubMed]
    [Google Scholar]
  31. Queiroz D. M. , Rocha G. A. , Rocha A. M. , Moura S. B. , Saraiva I. E. , Gomes L. I. , Soares T. F. , Melo F. F. , Cabral M. M. , Oliveira C. A. . ( 2011; ). dupA polymorphisms and risk of Helicobacter pylori-associated diseases. . Int J Med Microbiol 301:, 225–228. [CrossRef]
    [Google Scholar]
  32. Rahman M. , Mukhopadhyay A. K. , Nahar S. , Datta S. , Ahmad M. M. , Sarker S. , Masud I. M. , Engstrand L. , Albert M. J. . & other authors ( 2003; ). DNA-level characterization of Helicobacter pylori strains from patients with overt disease and with benign infections in Bangladesh. . J Clin Microbiol 41:, 2008–2014. [CrossRef] [PubMed]
    [Google Scholar]
  33. Rocha G. A. , Guerra J. B. , Rocha A. M. , Saraiva I. E. , da Silva D. A. , de Oliveira C. A. , Queiroz D. M. . ( 2005; ). IL1RN polymorphic gene and cagA-positive status independently increase the risk of noncardia gastric carcinoma. . Int J Cancer 115:, 678–683. [CrossRef] [PubMed]
    [Google Scholar]
  34. Santos A. , Queiroz D. M. , Ménard A. , Marais A. , Rocha G. A. , Oliveira C. A. , Nogueira A. M. , Uzeda M. , Mégraud F. . ( 2003; ). New pathogenicity marker found in the plasticity region of the Helicobacter pylori genome. . J Clin Microbiol 41:, 1651–1655. [CrossRef] [PubMed]
    [Google Scholar]
  35. Schmidt H. M. , Andres S. , Kaakoush N. O. , Engstrand L. , Eriksson L. , Goh K. L. , Fock K. M. , Hilmi I. D. , Dhamodaran S. . & other authors ( 2009; ). The prevalence of the duodenal ulcer promoting gene (dupA) in Helicobacter pylori isolates varies by ethnic group and is not universally associated with disease development: a case control study. . Gut Pathog 1:, 5. [CrossRef]
    [Google Scholar]
  36. Shiota S. , Matsunari O. , Watada M. , Hanada K. , Yamaoka Y. . ( 2010; ). Systematic review and meta-analysis: the relationship between the Helicobacter pylori dupA gene and clinical outcomes. . Gut Pathog 2:, 13. [CrossRef] [PubMed]
    [Google Scholar]
  37. Singh V. , Trikha B. , Nain C. K. , Singh K. , Vaiphei K. . ( 2002; ). Epidemiology of Helicobacter pylori and peptic ulcer in India. . J Gastroenterol Hepatol 17:, 659–665. [CrossRef] [PubMed]
    [Google Scholar]
  38. Tomb J. F. , White O. , Kerlavage A. R. , Clayton R. A. , Sutton G. G. , Fleischmann R. D. , Ketchum K. A. , Klenk H. P. , Gill S. . & other authors ( 1997; ). The complete genome sequence of the gastric pathogen Helicobacter pylori . . Nature 388:, 539–547. [CrossRef] [PubMed]
    [Google Scholar]
  39. Wotherspoon A. C. , Ortiz-Hidalgo C. , Falzon M. R. , Isaacson P. G. . ( 1991; ). Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. . Lancet 338:, 1175–1176. [CrossRef] [PubMed]
    [Google Scholar]
  40. Yakoob J. , Abbas Z. , Naz S. , Islam M. , Abid S. , Jafri W. . ( 2010; ). Associations between the plasticity region genes of Helicobacter pylori and gastroduodenal diseases in a high-prevalence area. . Gut Liver 4:, 345–350. [CrossRef] [PubMed]
    [Google Scholar]
  41. Yamaoka Y. , El-Zimaity H. M. T. , Gutierrez O. , Figura N. , Kim J. G. , Kodama T. , Kashima K. , Graham D. Y. . ( 1999; ). Relationship between the cagA 3′ repeat region of Helicobacter pylori, gastric histology, and susceptibility to low pH. . Gastroenterology 117:, 342–349. [CrossRef] [PubMed]
    [Google Scholar]
  42. Yamaoka Y. , Kwon D. H. , Graham D. Y. . ( 2000; ). A M( r) 34,000 proinflammatory outer membrane protein (oipA) of Helicobacter pylori. . Proc Natl Acad Sci U S A 97:, 7533–7538. [CrossRef] [PubMed]
    [Google Scholar]
  43. Yamaoka Y. , Kikuchi S. , el-Zimaity H. M. T. , Gutierrez O. , Osato M. S. , Graham D. Y. . ( 2002; ). Importance of Helicobacter pylori oipA in clinical presentation, gastric inflammation, and mucosal interleukin 8 production. . Gastroenterology 123:, 414–424. [CrossRef] [PubMed]
    [Google Scholar]
  44. Yamaoka Y. . ( 2008; ). Roles of the plasticity regions of Helicobacter pylori in gastroduodenal pathogenesis. . J Med Microbiol 57:, 545–553. [CrossRef] [PubMed]
    [Google Scholar]
  45. Zhang Z. , Zheng Q. , Chen X. , Xiao S. , Liu W. , Lu H. . ( 2008; ). The Helicobacter pylori duodenal ulcer promoting gene, dupA in China. . BMC Gastroenterol 8:, 49. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.038398-0
Loading
/content/journal/jmm/10.1099/jmm.0.038398-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error