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Abstract

The objectives of the study were to investigate the distribution of related genes () encoding a CphA metallo-β-lactamase (MBL) among 51 consecutive blood isolates and to compare different phenotypic methods for detecting CphA. The presence of was detected by PCR. Four phenotypic methods, the imipenem-EDTA combined disc test, imipenem-EDTA MBL Etest, agar dilution test and modified Hodge test (MHT), were used to detect imipenem susceptibility and MBL production. The results showed that 35 (69 %) blood isolates had All (100 %) of 16 isolates and 12 isolates, and 4 (80 %) of 5 isolates, carried , but none of 15 isolates did. With the standard inocula, irrespective of the presence or absence of , all but one (50, 98 %) isolates were susceptible to imipenem tested by disc diffusion, Etest and agar dilution (10 c.f.u. spot inocula), and did not exhibit MBL production by the imipenem-EDTA combined disc test and MBL Etest. By the agar dilution test using large inocula (10 c.f.u.), 34 (97 %) of 35 isolates had imipenem MICs of ≥16 µg ml, higher than the susceptible breakpoint (4 µg ml), and demonstrated positive results for the MHT, while one and all 17 isolates had imipenem MICs of ≤4 µg ml. In conclusion, the distribution of among aeromonads is species-specific, found in , and , and the MHT may be a phenotypic screening test for CphA production.

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2012-05-01
2020-07-14
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