1887

Abstract

The objectives of the study were to investigate the distribution of related genes () encoding a CphA metallo-β-lactamase (MBL) among 51 consecutive blood isolates and to compare different phenotypic methods for detecting CphA. The presence of was detected by PCR. Four phenotypic methods, the imipenem-EDTA combined disc test, imipenem-EDTA MBL Etest, agar dilution test and modified Hodge test (MHT), were used to detect imipenem susceptibility and MBL production. The results showed that 35 (69 %) blood isolates had All (100 %) of 16 isolates and 12 isolates, and 4 (80 %) of 5 isolates, carried , but none of 15 isolates did. With the standard inocula, irrespective of the presence or absence of , all but one (50, 98 %) isolates were susceptible to imipenem tested by disc diffusion, Etest and agar dilution (10 c.f.u. spot inocula), and did not exhibit MBL production by the imipenem-EDTA combined disc test and MBL Etest. By the agar dilution test using large inocula (10 c.f.u.), 34 (97 %) of 35 isolates had imipenem MICs of ≥16 µg ml, higher than the susceptible breakpoint (4 µg ml), and demonstrated positive results for the MHT, while one and all 17 isolates had imipenem MICs of ≤4 µg ml. In conclusion, the distribution of among aeromonads is species-specific, found in , and , and the MHT may be a phenotypic screening test for CphA production.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.038323-0
2012-05-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/5/712.html?itemId=/content/journal/jmm/10.1099/jmm.0.038323-0&mimeType=html&fmt=ahah

References

  1. Arakawa Y. , Shibata N. , Shibayama K. , Kurokawa H. , Yagi T. , Fujiwara H. , Goto M. . ( 2000; ). Convenient test for screening metallo-β-lactamase-producing gram-negative bacteria by using thiol compounds. . J Clin Microbiol 38:, 40–43.[PubMed]
    [Google Scholar]
  2. Aravena-Román M. , Harnett G. B. , Riley T. V. , Inglis T. J. , Chang B. J. . ( 2011; ). Aeromonas aquariorum is widely distributed in clinical and environmental specimens and can be misidentified as Aeromonas hydrophila . . J Clin Microbiol 49:, 3006–3008. [CrossRef] [PubMed]
    [Google Scholar]
  3. Balsalobre L. C. , Dropa M. , Lincopan N. , Mamizuka E. M. , Matté G. R. , Matté M. H. . ( 2009; ). Detection of metallo-β-lactamases-encoding genes in environmental isolates of Aeromonas hydrophila and Aeromonas jandaei . . Lett Appl Microbiol 49:, 142–145. [CrossRef] [PubMed]
    [Google Scholar]
  4. Brian J. D. , Julian K. . ( 2011; ). Medscape: Drug, Diseases & Procedures. Peritonitis and Abdominal Sepsis. . Available at http://emedicine.medscape.com/article/180234-overview#a0104. Updated on March 29, 2011.
  5. CLSI ( 2009; ). Performance Standards for Antimicrobial Susceptibility Testing; 19th Informational Supplement. M100-S19. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  6. CLSI ( 2010; ). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline M45-A2. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  7. Figueras M. J. , Alperi A. , Saavedra M. J. , Ko W. C. , Gonzalo N. , Navarro M. , Martínez-Murcia A. J. . ( 2009; ). Clinical relevance of the recently described species Aeromonas aquariorum . . J Clin Microbiol 47:, 3742–3746. [CrossRef] [PubMed]
    [Google Scholar]
  8. Fosse T. , Giraud-Morin C. , Madinier I. . ( 2003; ). [Phenotypes of beta-lactam resistance in the genus Aeromonas]. . Pathol Biol (Paris) 51:, 290–296 (in French). [CrossRef] [PubMed]
    [Google Scholar]
  9. Itoh H. , Kuwata G. , Tateyama S. , Yamashita K. , Inoue T. , Kataoka H. , Ido A. , Ogata K. , Takasaki M. . & other authors ( 1999; ). Aeromonas sobria infection with severe soft tissue damage and segmental necrotizing gastroenteritis in a patient with alcoholic liver cirrhosis. . Pathol Int 49:, 541–546. [CrossRef] [PubMed]
    [Google Scholar]
  10. Janda J. M. , Abbott S. L. . ( 2010; ). The genus Aeromonas: taxonomy, pathogenicity, and infection. . Clin Microbiol Rev 23:, 35–73. [CrossRef] [PubMed]
    [Google Scholar]
  11. Küpfer M. , Kuhnert P. , Korczak B. M. , Peduzzi R. , Demarta A. . ( 2006; ). Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences. . Int J Syst Evol Microbiol 56:, 2743–2751. [CrossRef] [PubMed]
    [Google Scholar]
  12. Lamy B. , Laurent F. , Kodjo A. . ( 2010; ). Validation of a partial rpoB gene sequence as a tool for phylogenetic identification of aeromonads isolated from environmental sources. . Can J Microbiol 56:, 217–228. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lee K. , Chong Y. , Shin H. B. , Kim Y. A. , Yong D. , Yum J. H. . ( 2001; ). Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobacter species. . Clin Microbiol Infect 7:, 88–91. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lee C. H. , Liu M. S. , Hsieh S. H. . ( 2003; ). Aeromonas hydrophila bacteremia presenting as non-traumatic acute osteomyelitis in a cirrhotic patient. . Chang Gung Med J 26:, 520–524.[PubMed]
    [Google Scholar]
  15. Lee C. C. , Chi C. H. , Lee N. Y. , Lee H. C. , Chen C. L. , Chen P. L. , Chang C. M. , Wu C. J. , Ko N. Y. . & other authors ( 2008; ). Necrotizing fasciitis in patients with liver cirrhosis: predominance of monomicrobial Gram-negative bacillary infections. . Diagn Microbiol Infect Dis 62:, 219–225. [CrossRef] [PubMed]
    [Google Scholar]
  16. Libisch B. , Giske C. G. , Kovács B. , Tóth T. G. , Füzi M. . ( 2008; ). Identification of the first VIM metallo-β-lactamase-producing multiresistant Aeromonas hydrophila strain. . J Clin Microbiol 46:, 1878–1880. [CrossRef] [PubMed]
    [Google Scholar]
  17. Martínez-Murcia A. J. , Saavedra M. J. , Mota V. R. , Maier T. , Stackebrandt E. , Cousin S. . ( 2008; ). Aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish. . Int J Syst Evol Microbiol 58:, 1169–1175. [CrossRef] [PubMed]
    [Google Scholar]
  18. Massidda O. , Rossolini G. M. , Satta G. . ( 1991; ). The Aeromonas hydrophila cphA gene: molecular heterogeneity among class B metallo-β-lactamases. . J Bacteriol 173:, 4611–4617.[PubMed]
    [Google Scholar]
  19. Mouës C. M. , Vos M. C. , van den Bemd G. J. , Stijnen T. , Hovius S. E. . ( 2004; ). Bacterial load in relation to vacuum-assisted closure wound therapy: a prospective randomized trial. . Wound Repair Regen 12:, 11–17. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mukhopadhyay C. , Bhargava A. , Ayyagari A. . ( 2003; ). Aeromonas hydrophila and aspiration pneumonia: a diverse presentation. . Yonsei Med J 44:, 1087–1090.[PubMed] [CrossRef]
    [Google Scholar]
  21. Neuwirth C. , Siebor E. , Robin F. , Bonnet R. . ( 2007; ). First occurrence of an IMP metallo-β-lactamase in Aeromonas caviae: IMP-19 in an isolate from France. . Antimicrob Agents Chemother 51:, 4486–4488. [CrossRef] [PubMed]
    [Google Scholar]
  22. Rossolini G. M. , Zanchi A. , Chiesurin A. , Amicosante G. , Satta G. , Guglielmetti P. . ( 1995; ). Distribution of cphA or related carbapenemase-encoding genes and production of carbapenemase activity in members of the genus Aeromonas . . Antimicrob Agents Chemother 39:, 346–349.[PubMed] [CrossRef]
    [Google Scholar]
  23. Rossolini G. M. , Walsh T. , Amicosante G. . ( 1996; ). The Aeromonas metallo-β-lactamases: genetics, enzymology, and contribution to drug resistance. . Microb Drug Resist 2:, 245–252. [CrossRef] [PubMed]
    [Google Scholar]
  24. Sánchez-Céspedes J. , Figueras M. J. , Aspiroz C. , Aldea M. J. , Toledo M. , Alperí A. , Marco F. , Vila J. . ( 2009; ). Development of imipenem resistance in an Aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis. . J Med Microbiol 58:, 451–455. [CrossRef] [PubMed]
    [Google Scholar]
  25. Segatore B. , Massidda O. , Satta G. , Setacci D. , Amicosante G. . ( 1993; ). High specificity of cphA-encoded metallo-β-lactamase from Aeromonas hydrophila AE036 for carbapenems and its contribution to β-lactam resistance. . Antimicrob Agents Chemother 37:, 1324–1328.[PubMed] [CrossRef]
    [Google Scholar]
  26. Talavera B. M. M. , Benassi F. O. , von Specht M. H. , Quiroga M. I. , García M. A. , Pucciarelli A. B. , Zubreski E. , Laczeski M. E. , Gutkind G. . ( 2006; ). Susceptibilities to carbapenems and presence of cphA gene on food-borne Aeromonas . . Braz Arch Biol Technol 49:, 677–682. [CrossRef]
    [Google Scholar]
  27. Walsh T. R. , Neville W. A. , Haran M. H. , Tolson D. , Payne D. J. , Bateson J. H. , MacGowan A. P. , Bennett P. M. . ( 1998; ). Nucleotide and amino acid sequences of the metallo-β-lactamase, ImiS, from Aeromonas veronii bv. sobria. . Antimicrob Agents Chemother 42:, 436–439.[PubMed]
    [Google Scholar]
  28. Wu C. J. , Wu J. J. , Yan J. J. , Lee H. C. , Lee N. Y. , Chang C. M. , Shih H. I. , Wu H. M. , Wang L. R. , Ko W. C. . ( 2007; ). Clinical significance and distribution of putative virulence markers of 116 consecutive clinical Aeromonas isolates in southern Taiwan. . J Infect 54:, 151–158. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wu C. J. , Lee H. C. , Chang T. T. , Chen C. Y. , Lee N. Y. , Chang C. M. , Sheu B. S. , Cheng P. N. , Shih H. I. , Ko W. C. . ( 2009; ). Aeromonas spontaneous bacterial peritonitis: a highly fatal infectious disease in patients with advanced liver cirrhosis. . J Formos Med Assoc 108:, 293–300. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yong D. , Lee K. , Yum J. H. , Shin H. B. , Rossolini G. M. , Chong Y. . ( 2002; ). Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. . J Clin Microbiol 40:, 3798–3801. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.038323-0
Loading
/content/journal/jmm/10.1099/jmm.0.038323-0
Loading

Data & Media loading...

Supplements

Supplementary Figure 

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error