1887

Abstract

In this study, 76 French and Tunisian urogenital specimens were subjected to molecular typing by using the two main molecular typing methods, the single nucleotide polymorphism (SNP) typing method and the combination analysis of a variable-number tandem-repeat (VNTR) marker in MG309 and SNP. Furthermore, we tried to develop a multiple-locus VNTR analysis (MLVA) method. The genome of G37 was analysed for VNTRs and four VNTRs were used for an MLVA. The method, applied directly on clinical specimens, was based on a analysis of VNTR loci labelled with fluorescent dyes by using multiplex PCR and capillary electrophoresis. This method had a 1.00 diversity index (DI) while the SNP typing and the combination of MG309 and SNPs had DIs of 0.853 and 0.989, respectively. However, among the sets of two concurrent specimens, taken at the same time from the urogenital tracts of 12 patients, only nine had matching MLVA profiles, while the two other methods gave identical profiles for all specimens amplified, except for one set. Moreover, eight new sequence types were described with the SNP typing method. The three molecular typing methods revealed a genetic heterogeneity, suggesting that was endemic in France and Tunisia and that the infections were not due to the clonal dissemination of one strain. Comparison of the typing results obtained with the three methods showed that the MLVA assay seemed too discriminatory to be used in future studies of sexual networks of infection. According to the discriminatory power and the feasibility of each -based method, we recommend that the analysis be used for general epidemiological studies and that the combination of MG309-STR and SNP methods should be used for sexual-network studies of infection.

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2012-04-01
2019-12-16
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