Detection of the quinolone protection gene and its prevalence in clinical isolates of in China Free

Abstract

The aim of this study was to detect novel variants of the gene family and analyse the prevalence of genes in clinical isolates of in China. In total, 442 clinical isolates of were collected from nine hospitals in four provinces in China. Antimicrobial susceptibility testing against six commonly used antibiotics was performed on these isolates. The sequences of the genes amplified by PCR were aligned with those of known genes in GenBank and an database. The resistance rate against co-trimoxazole was highest at 48.6 %, followed by resistance rates against ceftazidime, chloramphenicol, ticarcillin/clavulanate and tigecycline at 28.7, 21.3, 19.0 and 16.1 %, respectively. The highest susceptibility was shown to levofloxacin, with a resistance rate of just 6.1 %. genes were detected in 114 isolates, and comprised 11 previously identified genes and 20 new variants, bringing the total number of known genes to 47. The 20 novel genes were designated and the encoded proteins showed only 1–12 amino acid differences among each other. The most common genes in China were and its variant with prevalences of 17.5 % (20/114) and 13.2 % (15/114), respectively. Both the known and the novel genes were discovered in both quinolone non-sensitive and sensitive isolates with similar frequency, suggesting that the gene makes little contribution to quinolone resistance in this organism.

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2012-04-01
2024-03-28
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