1887

Abstract

The aim of this study was to detect novel variants of the gene family and analyse the prevalence of genes in clinical isolates of in China. In total, 442 clinical isolates of were collected from nine hospitals in four provinces in China. Antimicrobial susceptibility testing against six commonly used antibiotics was performed on these isolates. The sequences of the genes amplified by PCR were aligned with those of known genes in GenBank and an database. The resistance rate against co-trimoxazole was highest at 48.6 %, followed by resistance rates against ceftazidime, chloramphenicol, ticarcillin/clavulanate and tigecycline at 28.7, 21.3, 19.0 and 16.1 %, respectively. The highest susceptibility was shown to levofloxacin, with a resistance rate of just 6.1 %. genes were detected in 114 isolates, and comprised 11 previously identified genes and 20 new variants, bringing the total number of known genes to 47. The 20 novel genes were designated and the encoded proteins showed only 1–12 amino acid differences among each other. The most common genes in China were and its variant with prevalences of 17.5 % (20/114) and 13.2 % (15/114), respectively. Both the known and the novel genes were discovered in both quinolone non-sensitive and sensitive isolates with similar frequency, suggesting that the gene makes little contribution to quinolone resistance in this organism.

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2012-04-01
2019-12-11
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References

  1. Cavaco L. M., Hasman H., Xia S., Aarestrup F. M.. ( 2009;). qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin. . Antimicrob Agents Chemother 53:, 603–608. [CrossRef][PubMed]
    [Google Scholar]
  2. CLSI ( 2010;). Performance Standards for Antimicrobial Susceptibility Testing, 20th Informational Supplement. M100-S20. . Wayne, PA:; Clinical and Laboratory Standards Institute;.
  3. Gordon N. C., Wareham D. W.. ( 2010;). Novel variants of the Smqnr family of quinolone resistance genes in clinical isolates of Stenotrophomonas maltophilia. . J Antimicrob Chemother 65:, 483–489. [CrossRef][PubMed]
    [Google Scholar]
  4. Kim H. B., Park C. H., Kim C. J., Kim E. C., Jacoby G. A., Hooper D. C.. ( 2009;). Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period. . Antimicrob Agents Chemother 53:, 639–645. [CrossRef][PubMed]
    [Google Scholar]
  5. Krueger T. S., Clark E. A., Nix D. E.. ( 2001;). In vitro susceptibility of Stenotrophomonas maltophilia to various antimicrobial combinations. . Diagn Microbiol Infect Dis 41:, 71–78. [CrossRef][PubMed]
    [Google Scholar]
  6. Looney W. J., Narita M., Mühlemann K.. ( 2009;). Stenotrophomonas maltophilia: an emerging opportunist human pathogen. . Lancet Infect Dis 9:, 312–323. [CrossRef][PubMed]
    [Google Scholar]
  7. Martínez J. L., Alonso A., Gómez-Gómez J. M., Baquero F.. ( 1998;). Quinolone resistance by mutations in chromosomal gyrase genes. Just the tip of the iceberg?. J Antimicrob Chemother 42:, 683–688. [CrossRef][PubMed]
    [Google Scholar]
  8. Martínez-Martínez L., Pascual A., Jacoby G. A.. ( 1998;). Quinolone resistance from a transferable plasmid. . Lancet 351:, 797–799. [CrossRef][PubMed]
    [Google Scholar]
  9. Sánchez M. B., Martínez J. L.. ( 2010;). SmQnr contributes to intrinsic resistance to quinolones in Stenotrophomonas maltophilia. . Antimicrob Agents Chemother 54:, 580–581. [CrossRef][PubMed]
    [Google Scholar]
  10. Sánchez M. B., Hernández A., Rodríguez-Martínez J. M., Martínez-Martínez L., Martínez J. L.. ( 2008;). Predictive analysis of transmissible quinolone resistance indicates Stenotrophomonas maltophilia as a potential source of a novel family of Qnr determinants. . BMC Microbiol 8:, 148. [CrossRef][PubMed]
    [Google Scholar]
  11. Shimizu K., Kikuchi K., Sasaki T., Takahashi N., Ohtsuka M., Ono Y., Hiramatsu K.. ( 2008;). Smqnr, a new chromosome-carried quinolone resistance gene in Stenotrophomonas maltophilia. . Antimicrob Agents Chemother 52:, 3823–3825. [CrossRef][PubMed]
    [Google Scholar]
  12. Valdezate S., Vindel A., Echeita A., Baquero F., Cantó R.. ( 2002;). Topoisomerase II and IV quinolone resistance-determining regions in Stenotrophomonas maltophilia clinical isolates with different levels of quinolone susceptibility. . Antimicrob Agents Chemother 46:, 665–671. [CrossRef][PubMed]
    [Google Scholar]
  13. Valdezate S., Vindel A., Saéz-Nieto J. A., Baquero F., Cantón R.. ( 2005;). Preservation of topoisomerase genetic sequences during in vivo and in vitro development of high-level resistance to ciprofloxacin in isogenic Stenotrophomonas maltophilia strains. . J Antimicrob Chemother 56:, 220–223. [CrossRef][PubMed]
    [Google Scholar]
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