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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 61, Issue 1

Interlaboratory reproducibility of DiversiLab rep-PCR typing and clustering of isolates No Access

Abstract

We have investigated the reproducibility of DiversiLab rep-PCR fingerprints between two laboratories with the aim of determining if the fingerprints and clustering are laboratory-specific or portable. One-hundred non-duplicate isolates were used in this study. DNA isolation and rep-PCR were each performed separately in two laboratories and rep-PCR patterns generated in laboratory A were compared with those from laboratory B. Twelve isolates processed in laboratory A showed ≥98 % pattern similarity with the corresponding 12 isolates tested in laboratory B and were considered identical. Sixty-four isolates showed 95–97.9 % similarity with their corresponding isolates. Twenty-three isolates showed 90–94 % similarity with the corresponding isolates, while one isolate showed only 87.4 % similarity. However, intra-laboratory clustering was conserved: isolates that clustered in laboratory A also clustered in laboratory B. While clustering was conserved and reproducible at two different laboratories, demonstrating the robustness of rep-PCR, interlaboratory comparison of individual isolate fingerprints showed more variability. This comparison allows conclusions regarding clonality to be reached independent of the laboratory where the analysis is performed.

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© 2012 SGM
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2012-01-01
2025-12-12

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/content/journal/jmm/10.1099/jmm.0.036046-0
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Interlaboratory reproducibility of DiversiLab rep-PCR typing and clustering of Acinetobacter baumannii isolates
J. Med. Microbiol. 61, 137 (2012); https://doi.org/10.1099/jmm.0.036046-0
/content/journal/jmm/10.1099/jmm.0.036046-0
/content/journal/jmm/10.1099/jmm.0.036046-0
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