1887

Abstract

The autoinducer-2/LuxS signalling pathway participates in quorum sensing in diverse bacterial species. In group A streptococci (GAS), LuxS has been shown to be involved in regulating the expression of several important virulence factors. Streptococcal pyrogenic exotoxin B (SpeB), a cysteine protease that has important roles in GAS pathogenesis, is positively regulated by LuxS in M3 and M5 strains. In the present study, it was found that the supernatant harvested from an overnight culture stimulated M1 strains to express . However, mutation of the gene in M1 strains or treating M1 strains with mutant culture supernatant did not affect expression, indicating that the LuxS pathway is not involved in regulation of expression in M1 strains. In addition, the acid property of culture broth was found to be able to stimulate M1 strains to express in the same LuxS-independent manner. These results indicate that expression in M1 strains is induced by environmental pH changes but is not regulated by the LuxS signalling pathway.

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2012-01-01
2019-10-15
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