1887

Abstract

A total of 143 group B streptococcus (GBS) isolates collected from mothers at the Maternity Hospital in Kuwait were investigated for their serotypes and antibiotic resistance, and screened by PCR for the carriage of genes for resistance to tetracycline (, , , ), erythromycin (, , , , , , , ) and aminoglycosides (, , . All isolates were serotyped using a latex agglutination test. Most of the isolates belonged to serotypes V (38.5 %), III (20.9 %), Ia (7.7 %) and II (11.2 %). Sixteen isolates (11.2 %) were nontypable. All isolates were susceptible to penicillin, ampicillin and cefotaxime (MICs 0.016–0.094 µg ml) but were resistant to trimethoprim (92.3 %), tetracycline (89.5 %), minocycline (89.5 %), high-level kanamycin (76.9 %), chloramphenicol (30.0 %), erythromycin (12.6 %), clindamycin (7.0 %), high-level streptomycin (3.5 %) and ciprofloxacin (0.7 %). The tetracycline-resistant isolates contained (94.5 %), (3.9 %), (1.6 %) and (0.8 %). The erythromycin-resistant isolates contained (61.1 %), (38.9 %), (5.5 %), (5.5 %) and (11 %). All high-level kanamycin-resistant isolates contained One of the high-level streptomycin-resistant isolates contained . Partial DNA sequencing of revealed sequences with 99 % similarity to found in , , and , suggesting that the GBS isolates could have acquired from other Gram-positive cocci. The high proportion of isolates with resistance to tetracycline, high-level kanamycin and trimethoprim, and the increase in the prevalence of erythromycin resistance, represents an emerging public health concern that needs further surveillance.

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2012-01-01
2019-12-06
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