1887

Abstract

The development of gonococcal point-of-care tests (POCTs) has been challenging due to the relatively monomorphic nature of the genus. The BioStar Optical ImmunoAssay (OIA) POCT for diagnosing infection detects a specific epitope on the L7/L12 ribosomal protein, which reduces cross-reactivity with other neisseriae, and produces a highly specific test. A laboratory-based evaluation of this POCT was performed to determine its analytical sensitivity and specificity. A panel of ( = 158) and non-gonococcal ( = 62) isolates were examined. The OIA GC POCT positively reacted with 99.4 % of isolates and produced no reaction with 88.7 % of non-gonococcal isolates. It cross-reacted with six strains of and one non-speciated sp., but failed to produce a positive result with one isolate of The OIA GC POCT required a bacterial suspension of ~6.4×10 c.f.u. ml and ~6.2×10 c.f.u. ml to produce a reactive result. The OIA POCT detected the majority of (99.4 %) isolates examined.

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2011-12-01
2020-01-19
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