1887

Abstract

This study was conducted to detect and analyse the presence of plasmid-mediated quinolone resistance (PMQR) determinants [, and ] among isolates from patients in Anhui province, PR China. During 2009–2010, 31 strains were collected from various hospital units and patient specimens. Using PCR, genes were detected in eight isolates, but and genes were not found. The genes , , , , and were present in 6.5, 3.2, 6.5, 3.2, 3.2 and 3.2 % of isolates, respectively. A new subgene of variant () was found and identified for what we believe to be the first time. PFGE after I digestion of genomic DNA indicated that -positive strains were not clonally related. Conjugation experiments were conducted to determine whether the -carrying plasmids were self-transferable, and plasmids of transconjugants were extracted and analysed. The genes were transferred from three clinical isolates to their transconjugants. Two genes transferred quinolone resistance with a plasmid of ~11 kb, whilst the size of the plasmid carrying the gene was ~64 kb. The susceptibility of positive isolates and transconjugants was tested using an agar dilution method according to Clinical and Laboratory Standards Institute guidelines, and the MICs of ciprofloxacin and levofloxacin were determined using Etest strips. Most isolates with genes were resistant to fluoroquinolones and other antimicrobial agents. The MICs of transconjugants showed reduced susceptibility to fluoroquinolones.

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2011-12-01
2019-10-23
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