1887

Abstract

Spacer sequences of 42 and 127 bp, respectively, between the IS insertion sequence and have been observed in . However, it is not known whether different spacers upstream of and a promoter within the 127 bp spacer influence cephalosporin resistance. Three recombinant plasmids with different spacers and with or without IS were constructed to compare expression and susceptibility to cephalosporins. Our experiment revealed enhanced expression and a relatively high level resistance to cefotaxime and cefepime in recombinant plasmid IS42CTX-M-3, which contained IS and the 42 bp spacer. A minor difference in expression was observed in recombinants IS127CTX-M-3 and 127CTX-M-3, which contained a 127 bp spacer with or without IS, respectively. In conclusion, the promoter within IS and a shorter spacer (42 bp but not 127 bp) between IS and are necessary for high-level expression.

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2011-12-01
2019-10-23
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References

  1. Bonnet R. . ( 2004; ). Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. . Antimicrob Agents Chemother 48:, 1–14. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bonnet R. , Dutour C. , Sampaio J. L. , Chanal C. , Sirot D. , Labia R. , De Champs C. , Sirot J. . ( 2001; ). Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp-240→Gly. . Antimicrob Agents Chemother 45:, 2269–2275. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bradford M. M. . ( 1976; ). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. . Anal Biochem 72:, 248–254. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cantón R. , Coque T. M. . ( 2006; ). The CTX-M beta-lactamase pandemic. . Curr Opin Microbiol 9:, 466–475. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cao V. , Lambert T. , Courvalin P. . ( 2002; ). ColE1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum beta-lactamase CTX-M-17. . Antimicrob Agents Chemother 46:, 1212–1217. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cattoir V. , Nordmann P. , Silva-Sanchez J. , Espinal P. , Poirel L. . ( 2008; ). ISEcp1-mediated transposition of qnrB-like gene in Escherichia coli . . Antimicrob Agents Chemother 52:, 2929–2932. [CrossRef] [PubMed]
    [Google Scholar]
  7. CLSI ( 2006; ). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard. M7-A7. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  8. CLSI ( 2007; ). Performance Standards for Antimicrobial Susceptibility Testing, 17th Informational Supplement. M100-S17. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  9. Eckert C. , Gautier V. , Arlet G. . ( 2006; ). DNA sequence analysis of the genetic environment of various bla CTX-M genes. . J Antimicrob Chemother 57:, 14–23. [CrossRef] [PubMed]
    [Google Scholar]
  10. Gołebiewski M. , Kern-Zdanowicz I. , Zienkiewicz M. , Adamczyk M. , Zylinska J. , Baraniak A. , Gniadkowski M. , Bardowski J. , Cegłowski P. . ( 2007; ). Complete nucleotide sequence of the pCTX-M3 plasmid and its involvement in spread of the extended-spectrum beta-lactamase gene bla CTX-M-3 . . Antimicrob Agents Chemother 51:, 3789–3795. [CrossRef] [PubMed]
    [Google Scholar]
  11. Haldorsen B. , Aasnaes B. , Dahl K. H. , Hanssen A. M. , Simonsen G. S. , Walsh T. R. , Sundsfjord A. , Lundblad E. W. . ( 2008; ). The AmpC phenotype in Norwegian clinical isolates of Escherichia coli is associated with an acquired ISEcp1-like ampC element or hyperproduction of the endogenous AmpC. . J Antimicrob Chemother 62:, 694–702. [CrossRef] [PubMed]
    [Google Scholar]
  12. Karim A. , Poirel L. , Nagarajan S. , Nordmann P. . ( 2001; ). Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1 . . FEMS Microbiol Lett 201:, 237–241.[PubMed]
    [Google Scholar]
  13. Lartigue M. F. , Poirel L. , Nordmann P. . ( 2004; ). Diversity of genetic environment of bla CTX-M genes. . FEMS Microbiol Lett 234:, 201–207. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lewin B. . ( 2006; ). Promoter recognition depends on consensus sequences. . In Genes IX, chapter 11.12, pp. 272–274. Sudbury, MA: Jones & Bartlett Publishers.
  15. Ma L. , Lin C. J. , Chen J. H. , Fung C. P. , Chang F. Y. , Lai Y. K. , Lin J. C. , Siu L. K. . the Taiwan Surveillance of Antimicrobial Resistance Project ( 2009; ). Widespread dissemination of aminoglycoside resistance genes armA and rmtB in Klebsiella pneumoniae isolates in Taiwan producing CTX-M-type extended-spectrum beta-lactamases. . Antimicrob Agents Chemother 53:, 104–111. [CrossRef] [PubMed]
    [Google Scholar]
  16. Messai Y. , Iabadene H. , Benhassine T. , Alouache S. , Tazir M. , Gautier V. , Arlet G. , Bakour R. . ( 2008; ). Prevalence and characterization of extended-spectrum beta-lactamases in Klebsiella pneumoniae in Algiers hospitals (Algeria). . Pathol Biol (Paris) 56:, 319–325.[PubMed] [CrossRef]
    [Google Scholar]
  17. Núñez C. , Adams L. , Childers S. , Lovley D. R. . ( 2004; ). The RpoS sigma factor in the dissimilatory Fe(III)-reducing bacterium Geobacter sulfurreducens . . J Bacteriol 186:, 5543–5546. [CrossRef] [PubMed]
    [Google Scholar]
  18. Ping Y. , Ogawa W. , Kuroda T. , Tsuchiya T. . ( 2007; ). Gene cloning and characterization of KdeA, a multidrug efflux pump from Klebsiella pneumoniae . . Biol Pharm Bull 30:, 1962–1964. [CrossRef] [PubMed]
    [Google Scholar]
  19. Poirel L. , Decousser J. W. , Nordmann P. . ( 2003; ). Insertion sequence ISEcp1B is involved in expression and mobilization of a bla CTX-M beta-lactamase gene. . Antimicrob Agents Chemother 47:, 2938–2945. [CrossRef] [PubMed]
    [Google Scholar]
  20. Poirel L. , Lartigue M. F. , Decousser J. W. , Nordmann P. . ( 2005; ). ISEcp1B-mediated transposition of bla CTX-M in Escherichia coli . . Antimicrob Agents Chemother 49:, 447–450. [CrossRef] [PubMed]
    [Google Scholar]
  21. Rodríguez M. M. , Power P. , Radice M. , Vay C. , Famiglietti A. , Galleni M. , Ayala J. A. , Gutkind G. . ( 2004; ). Chromosome-encoded CTX-M-3 from Kluyvera ascorbata: a possible origin of plasmid-borne CTX-M-1-derived cefotaximases. . Antimicrob Agents Chemother 48:, 4895–4897. [CrossRef] [PubMed]
    [Google Scholar]
  22. Rossolini G. M. , D’Andrea M. M. , Mugnaioli C. . ( 2008; ). The spread of CTX-M-type extended-spectrum beta-lactamases. . Clin Microbiol Infect 14: (Suppl. 1), 33–41. [CrossRef] [PubMed]
    [Google Scholar]
  23. Saladin M. , Cao V. T. , Lambert T. , Donay J. L. , Herrmann J. L. , Ould-Hocine Z. , Verdet C. , Delisle F. , Philippon A. , Arlet G. . ( 2002; ). Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. . FEMS Microbiol Lett 209:, 161–168.[PubMed]
    [Google Scholar]
  24. Siu L. K. , Ho P. L. , Yuen K. Y. , Wong S. S. , Chau P. Y. . ( 1997; ). Transferable hyperproduction of TEM-1 beta-lactamase in Shigella flexneri due to a point mutation in the Pribnow box. . Antimicrob Agents Chemother 41:, 468–470.[PubMed]
    [Google Scholar]
  25. Tamang M. D. , Nam H. M. , Kim T. S. , Jang G. C. , Jung S. C. , Lim S. K. . ( 2011; ). Emergence of extended-spectrum β-lactamase (CTX-M-15 and CTX-M-14)-producing nontyphoid Salmonella with reduced susceptibility to ciprofloxacin among food animals and humans in Korea. . J Clin Microbiol 49:, 2671–2675. [CrossRef] [PubMed]
    [Google Scholar]
  26. Turton J. F. , Ward M. E. , Woodford N. , Kaufmann M. E. , Pike R. , Livermore D. M. , Pitt T. L. . ( 2006; ). The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii . . FEMS Microbiol Lett 258:, 72–77. [CrossRef] [PubMed]
    [Google Scholar]
  27. Verdet C. , Gautier V. , Chachaty E. , Ronco E. , Hidri N. , Decré D. , Arlet G. . ( 2009; ). Genetic context of plasmid-carried bla CMY-2-like genes in Enterobacteriaceae . . Antimicrob Agents Chemother 53:, 4002–4006. [CrossRef] [PubMed]
    [Google Scholar]
  28. Wachino J. , Yamane K. , Kimura K. , Shibata N. , Suzuki S. , Ike Y. , Arakawa Y. . ( 2006; ). Mode of transposition and expression of 16S rRNA methyltransferase gene rmtC accompanied by ISEcp1 . . Antimicrob Agents Chemother 50:, 3212–3215. [CrossRef] [PubMed]
    [Google Scholar]
  29. Woodford N. , Ward M. E. , Kaufmann M. E. , Turton J. , Fagan E. J. , James D. , Johnson A. P. , Pike R. , Warner M. et al. & other authors ( 2004; ). Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK. . J Antimicrob Chemother 54:, 735–743. [CrossRef] [PubMed]
    [Google Scholar]
  30. Xu X. , Wu S. , Ye X. , Liu Y. , Shi W. , Zhang Y. , Wang M. . ( 2007; ). Prevalence and expression of the plasmid-mediated quinolone resistance determinant qnrA1 . . Antimicrob Agents Chemother 51:, 4105–4110. [CrossRef] [PubMed]
    [Google Scholar]
  31. Yu W. L. , Chuang Y. C. , Walther-Rasmussen J. . ( 2006; ). Extended-spectrum beta-lactamases in Taiwan: epidemiology, detection, treatment and infection control. . J Microbiol Immunol Infect 39:, 264–277.[PubMed]
    [Google Scholar]
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