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1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 60, Issue 12

Effect of spacer sequences between and IS on expression No Access

Abstract

Spacer sequences of 42 and 127 bp, respectively, between the IS insertion sequence and have been observed in . However, it is not known whether different spacers upstream of and a promoter within the 127 bp spacer influence cephalosporin resistance. Three recombinant plasmids with different spacers and with or without IS were constructed to compare expression and susceptibility to cephalosporins. Our experiment revealed enhanced expression and a relatively high level resistance to cefotaxime and cefepime in recombinant plasmid IS42CTX-M-3, which contained IS and the 42 bp spacer. A minor difference in expression was observed in recombinants IS127CTX-M-3 and 127CTX-M-3, which contained a 127 bp spacer with or without IS, respectively. In conclusion, the promoter within IS and a shorter spacer (42 bp but not 127 bp) between IS and are necessary for high-level expression.

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© 2011 SGM
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2011-12-01
2025-12-13

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/content/journal/jmm/10.1099/jmm.0.033910-0
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Effect of spacer sequences between blaCTX-M and ISEcp1 on blaCTX-M expression
J. Med. Microbiol. 60, 1787 (2011); https://doi.org/10.1099/jmm.0.033910-0
/content/journal/jmm/10.1099/jmm.0.033910-0
/content/journal/jmm/10.1099/jmm.0.033910-0
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