1887

Abstract

species have emerged as opportunistic nosocomial pathogens in intensive care units. Epidemic spread and outbreaks of multidrug-resistant or carbapenem-resistant infections have been described worldwide. Species distribution, antimicrobial resistance and genotypes were investigated for species isolates collected from a single institution in Korea over 7 years. Two hundred and eighty-seven species isolates were collected from patients with bloodstream infections in one Korean hospital from 2003 to 2010. Most of them belonged to the complex (94.4 %). The most frequently isolated species was (44.2 %), followed by (formerly genomic species 13TU) (34.1 %). The proportion of increased significantly from 2008 to 2010 (40.4 to 50.0 %). From 2008, imipenem and meropenem resistance rates increased significantly compared with 2003–2007 (12.9 % and 20.5 %, respectively, to 41.4 % and 41.5 %, respectively). An increased carbapenem resistance rate between the two periods was identified more clearly amongst isolates. Polymyxin-resistant isolates emerged in 2008–2010, despite the availability of few isolates. The increase of carbapenem resistance in might be due to the substitution of main clones. Although ST92 and ST69 were the most prevalent clones amongst in 2003–2007 (47.8 % and 15.9 %, respectively), ST75 and ST138 had increased in 2008–2010 (39.7 % and 25.9 %, respectively). Although ST92 showed moderate resistance to carbapenems, most ST75 and ST138 isolates were resistant to carbapenems. All ST75 and ST138 isolates, but only one ST92 isolate, contained the gene. Increased carbapenem resistance in species and isolates might be due to the expansion of specific carbapenem-resistant clones.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.033852-0
2012-01-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/61/1/71.html?itemId=/content/journal/jmm/10.1099/jmm.0.033852-0&mimeType=html&fmt=ahah

References

  1. Bartual S. G. , Seifert H. , Hippler C. , Luzon M. A. , Wisplinghoff H. , Rodríguez-Valera F. . ( 2005; ). Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii . . J Clin Microbiol 43:, 4382–4390. [CrossRef] [PubMed]
    [Google Scholar]
  2. Boo T. W. , Walsh F. , Crowley B. . & other authors ( 2009; ). Molecular characterization of carbapenem-resistant Acinetobacter species in an Irish university hospital: predominance of Acinetobacter genomic species 3. . J Med Microbiol 58:, 209–216. [CrossRef] [PubMed]
    [Google Scholar]
  3. CLSI ( 2009; ). Performance Standards for Antimicrobial Susceptibility Testing; 19th Informational Supplement. M100-S19. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  4. Diancourt L. , Passet V. , Nemec A. , Dijkshoorn L. , Brisse S. . ( 2010; ). The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool. . PLoS ONE 5:, e10034. [CrossRef] [PubMed]
    [Google Scholar]
  5. Dijkshoorn L. , Aucken H. , Gerner-Smidt P. , Janssen P. , Kaufmann M. E. , Garaizar J. , Ursing J. , Pitt T. L. . ( 1996; ). Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. . J Clin Microbiol 34:, 1519–1525.[PubMed]
    [Google Scholar]
  6. Dijkshoorn L. , Nemec A. , Seifert H. . ( 2007; ). An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii . . Nat Rev Microbiol 5:, 939–951. [CrossRef] [PubMed]
    [Google Scholar]
  7. Donnarumma F. , Sergi S. , Indorato C. , Mastromei G. , Monnanni R. , Nicoletti P. , Pecile P. , Cecconi D. , Mannino R. et al. ( 2010; ). Molecular characterization of Acinetobacter isolates collected in intensive care units of six hospitals in Florence, Italy, during a 3-year surveillance program: a population structure analysis. . J Clin Microbiol 48:, 1297–1304. [CrossRef] [PubMed]
    [Google Scholar]
  8. Enright M. C. , Spratt B. G. . ( 1999; ). Extensive variation in the ddl gene of penicillin-resistant Streptococcus pneumoniae results from a hitchhiking effect driven by the penicillin-binding protein 2b gene. . Mol Biol Evol 16:, 1687–1695.[PubMed] [CrossRef]
    [Google Scholar]
  9. Espinal P. , Roca I. , Vila J. . ( 2011; ). Clinical impact and molecular basis of antimicrobial resistance in non-baumannii Acinetobacter . . Future Microbiol 6:, 495–511. [CrossRef] [PubMed]
    [Google Scholar]
  10. Feil E. J. , Smith J. M. , Enright M. C. , Spratt B. G. . ( 2000; ). Estimating recombinational parameters in Streptococcus pneumoniae from multilocus sequence typing data. . Genetics 154:, 1439–1450.[PubMed]
    [Google Scholar]
  11. Feil E. J. , Cooper J. E. , Grundmann H. , Robinson D. A. , Enright M. C. , Berendt T. , Peacock S. J. , Smith J. M. , Murphy M. . & other authors ( 2003; ). How clonal is Staphylococcus aureus?. J Bacteriol 185:, 3307–3316. [CrossRef] [PubMed]
    [Google Scholar]
  12. Feil E. J. , Li B. C. , Aanensen D. M. , Hanage W. P. , Spratt B. G. . ( 2004; ). eburst: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. . J Bacteriol 186:, 1518–1530. [CrossRef] [PubMed]
    [Google Scholar]
  13. Gales A. C. , Jones R. N. , Sader H. S. . ( 2006; ). Global assessment of the antimicrobial activity of polymyxin B against 54 731 clinical isolates of Gram-negative bacilli: report from the SENTRY antimicrobial surveillance programme (2001-2004). . Clin Microbiol Infect 12:, 315–321. [CrossRef] [PubMed]
    [Google Scholar]
  14. Giannouli M. , Cuccurullo S. , Crivaro V. , Di Popolo A. , Bernardo M. , Tomasone F. , Amato G. , Brisse S. , Triassi M. . & other authors ( 2010; ). Molecular epidemiology of multidrug-resistant Acinetobacter baumannii in a tertiary care hospital in Naples, Italy, shows the emergence of a novel epidemic clone. . J Clin Microbiol 48:, 1223–1230. [CrossRef] [PubMed]
    [Google Scholar]
  15. Hamouda A. , Evans B. A. , Towner K. J. , Amyes S. G. . ( 2010; ). Characterization of epidemiologically unrelated Acinetobacter baumannii isolates from four continents by use of multilocus sequence typing, pulsed-field gel electrophoresis, and sequence-based typing of bla OXA-51-like genes. . J Clin Microbiol 48:, 2476–2483. [CrossRef] [PubMed]
    [Google Scholar]
  16. Hujer K. M. , Hujer A. M. , Hulten E. A. , Bajaksouzian S. , Adams J. M. , Donskey C. J. , Ecker D. J. , Massire C. , Eshoo M. W. . & other authors ( 2006; ). Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. . Antimicrob Agents Chemother 50:, 4114–4123. [CrossRef] [PubMed]
    [Google Scholar]
  17. Karah N. , Haldorsen B. , Hegstad K. , Simonsen G. S. , Sundsfjord A. , Samuelsen Ø. . on behalf of the Norwegian Study Group of Acinetobacter ( 2011; ). Species identification and molecular characterization of Acinetobacter spp. blood culture isolates from Norway. . J Antimicrob Chemother 66:, 738–744. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kim C. K. , Lee Y. , Lee H. , Woo G. J. , Song W. , Kim M. N. , Lee W. G. , Jeong S. H. , Lee K. , Chong Y. . ( 2010; ). Prevalence and diversity of carbapenemases among imipenem-nonsusceptible Acinetobacter isolates in Korea: emergence of a novel OXA-182. . Diagn Microbiol Infect Dis 68:, 432–438. [CrossRef] [PubMed]
    [Google Scholar]
  19. Ko K. S. , Suh J. Y. , Kwon K. T. , Jung S. I. , Park K. H. , Kang C. I. , Chung D. R. , Peck K. R. , Song J. H. . ( 2007; ). High rates of resistance to colistin and polymyxin B in subgroups of Acinetobacter baumannii isolates from Korea. . J Antimicrob Chemother 60:, 1163–1167. [CrossRef] [PubMed]
    [Google Scholar]
  20. La Scola B. , Gundi V. A. , Khamis A. , Raoult D. . ( 2006; ). Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species. . J Clin Microbiol 44:, 827–832. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lee K. , Lim C. H. , Cho J. H. , Lee W. G. , Uh Y. , Kim H. J. , Yong D. , Chong Y. . the KONSAR Group ( 2006; ). High prevalence of ceftazidime-resistant Klebsiella pneumoniae and increase of imipenem-resistant Pseudomonas aeruginosa and Acinetobacter spp. in Korea: a KONSAR program in 2004. . Yonsei Med J 47:, 634–645. [CrossRef] [PubMed]
    [Google Scholar]
  22. Lee J. H. , Choi C. H. , Kang H. Y. , Lee J. Y. , Kim J. , Lee Y. C. , Seol S. Y. , Cho D. T. , Kim K. W. . & other authors ( 2007; ). Differences in phenotypic and genotypic traits against antimicrobial agents between Acinetobacter baumannii and Acinetobacter genomic species 13TU. . J Antimicrob Chemother 59:, 633–639. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mugnier P. D. , Poirel L. , Naas T. , Nordmann P. . ( 2010; ). Worldwide dissemination of the bla OXA-23 carbapenemase gene of Acinetobacter baumannii . . Emerg Infect Dis 16:, 35–40. [CrossRef] [PubMed]
    [Google Scholar]
  24. Nation R. L. , Li J. . ( 2009; ). Colistin in the 21st century. . Curr Opin Infect Dis 22:, 535–543. [CrossRef] [PubMed]
    [Google Scholar]
  25. Nemec A. , Krizova L. , Maixnerova M. , van der Reijden T. J. , Deschaght P. , Passet V. , Vaneechoutte M. , Brisse S. , Dijkshoorn L. . ( 2011; ). Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). . Res Microbiol 162:, 393–404. [CrossRef] [PubMed]
    [Google Scholar]
  26. Park Y. K. , Choi J. Y. , Jung S. I. , Park K. H. , Lee H. , Jung D. S. , Heo S. T. , Kim S. W. , Chang H. H. . & other authors ( 2009a; ). Two distinct clones of carbapenem-resistant Acinetobacter baumannii isolates from Korean hospitals. . Diagn Microbiol Infect Dis 64:, 389–395. [CrossRef]
    [Google Scholar]
  27. Park Y. K. , Jung S. I. , Park K. H. , Cheong H. S. , Peck K. R. , Song J. H. , Ko K. S. . ( 2009b; ). Independent emergence of colistin-resistant Acinetobacter spp. isolates from Korea. . Diagn Microbiol Infect Dis 64:, 43–51. [CrossRef] [PubMed]
    [Google Scholar]
  28. Park Y. K. , Lee G. H. , Baek J. Y. , Chung D. R. , Peck K. R. , Song J. H. , Ko K. S. . ( 2010; ). A single clone of Acinetobacter baumannii, ST22, is responsible for high antimicrobial resistance rates of Acinetobacter spp. isolates that cause bacteremia and urinary tract infections in Korea. . Microb Drug Resist 16:, 143–149. [CrossRef] [PubMed]
    [Google Scholar]
  29. Paterson D. L. , Doi Y. . ( 2007; ). A step closer to extreme drug resistance (XDR) in gram-negative bacilli. . Clin Infect Dis 45:, 1179–1181. [CrossRef] [PubMed]
    [Google Scholar]
  30. Peleg A. Y. , Seifert H. , Paterson D. L. . ( 2008; ). Acinetobacter baumannii: emergence of a successful pathogen. . Clin Microbiol Rev 21:, 538–582. [CrossRef] [PubMed]
    [Google Scholar]
  31. Perez F. , Hujer A. M. , Hujer K. M. , Decker B. K. , Rather P. N. , Bonomo R. A. . ( 2007; ). Global challenge of multidrug-resistant Acinetobacter baumannii . . Antimicrob Agents Chemother 51:, 3471–3484. [CrossRef] [PubMed]
    [Google Scholar]
  32. Turton J. F. , Shah J. , Ozongwu C. , Pike R. . ( 2010; ). Incidence of Acinetobacter species other than A. baumannii among clinical isolates of Acinetobacter: evidence for emerging species. . J Clin Microbiol 48:, 1445–1449. [CrossRef] [PubMed]
    [Google Scholar]
  33. van den Broek P. J. , van der Reijden T. J. K. , van Strijen E. , Helmig-Schurter A. V. , Bernards A. T. , Dijkshoorn L. . ( 2009; ). Endemic and epidemic Acinetobacter species in a university hospital: an 8-year survey. . J Clin Microbiol 47:, 3593–3599. [CrossRef] [PubMed]
    [Google Scholar]
  34. van Dessel H. , Dijkshoorn L. , van der Reijden T. , Bakker N. , Paauw A. , van den Broek P. , Verhoef J. , Brisse S. . ( 2004; ). Identification of a new geographically widespread multiresistant Acinetobacter baumannii clone from European hospitals. . Res Microbiol 155:, 105–112. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wisplinghoff H. , Hippler C. , Bartual S. G. , Haefs C. , Stefanik D. , Higgins P. G. , Seifert H. . ( 2008; ). Molecular epidemiology of clinical Acinetobacter baumannii and Acinetobacter genomic species 13TU isolates using a multilocus sequencing typing scheme. . Clin Microbiol Infect 14:, 708–715. [CrossRef] [PubMed]
    [Google Scholar]
  36. Woodford N. , Ellington M. J. , Coelho J. M. , Turton J. F. , Ward M. E. , Brown S. , Amyes S. G. , Livermore D. M. . ( 2006; ). Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. . Int J Antimicrob Agents 27:, 351–353. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.033852-0
Loading
/content/journal/jmm/10.1099/jmm.0.033852-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error