1887

Abstract

is a major pathogen of respiratory infections that utilizes platelet-activating factor receptor (PAFR) for firm adherence to host cells. The mucolytic agent -carboxymethylcysteine (-CMC) has been shown to exert inhibitory effects against infection by several respiratory pathogens including and . Moreover, clinical studies have implicated the benefits of -CMC in preventing exacerbation of chronic obstructive pulmonary disease, which is considered to be related to respiratory infections. In this study, to assess whether the potency of -CMC is attributable to inhibition of pneumococcal adherence to host cells, an alveolar epithelial cell line stimulated with interleukin-1α was used as a model of inflamed epithelial cells. Despite upregulation of PAFR by inflammatory activation, treatment with -CMC efficiently inhibited pneumococcal adherence to host epithelial cells. In order to gain insight into the inhibitory mechanism, the effects of -CMC on PAFR expression were also investigated. Following treatment with -CMC, PAFR expression was reduced at both mRNA and post-transcriptional levels. Interestingly, -CMC was also effective in inhibiting pneumococcal adherence to cells transfected with PAFR small interfering RNAs. These results indicate -CMC as a probable inhibitor targeting numerous epithelial receptors that interact with .

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2012-01-01
2020-07-07
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