1887

Abstract

Brucellosis is a worldwide zoonotic disease that often requires serology for diagnosis. The serum agglutination test is the gold standard assay, but ELISAs are used by many laboratories. Many commercial ELISAs are available, but few studies have compared their performance. This study compared the ability of four commercially available ELISA kits (from Bio-Quant, Immuno-Biological Laboratories – America, Vircell and Euroimmun) to diagnose brucellosis in patients from Egypt and the USA. The sensitivities for all kits tested, except the Vircell kit, were >90 %, whilst the specificities were variable, with the Bio-Quant assay having a specificity of <40 %. Detection of IgG antibody was more sensitive than detection of IgM antibody for diagnosing brucellosis cases, but the specificity was comparable. Overall, there was good agreement between all of the kits except for the Bio-Quant kit. None of the diagnostic assays was 100 % reliable for diagnosing brucellosis; therefore, serology results need to be considered in tandem with patient history, clinical signs and other test results.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.033381-0
2011-12-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/12/1767.html?itemId=/content/journal/jmm/10.1099/jmm.0.033381-0&mimeType=html&fmt=ahah

References

  1. Afifi S., Earhart K., Azab M. A., Youssef F. G., El Sakka H., Wasfy M., Mansour H., El Oun S., Rakha M., Mahoney F.. ( 2005;). Hospital-based surveillance for acute febrile illness in Egypt: a focus on community-acquired bloodstream infections. . Am J Trop Med Hyg 73:, 392–399.[PubMed]
    [Google Scholar]
  2. Al Dahouk S., Tomaso H., Nöckler K., Neubauer H., Frangoulidis D.. ( 2003;). Laboratory-based diagnosis of brucellosis – a review of the literature. Part II: serological tests for brucellosis. . Clin Lab 49:, 577–589.[PubMed]
    [Google Scholar]
  3. Araj G. F.. ( 1999;). Human brucellosis: a classical infectious disease with persistent diagnostic challenges. . Clin Lab Sci 12:, 207–212.[PubMed]
    [Google Scholar]
  4. Araj G. F., Lulu A. R., Mustafa M. Y., Khateeb M. I.. ( 1986a;). Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. . J Hyg (Lond) 97:, 457–469. [CrossRef][PubMed]
    [Google Scholar]
  5. Araj G. F., Lulu A. R., Saadah M. A., Mousa A. M., Strannegard I. L., Shakir R. A.. ( 1986b;). Rapid diagnosis of central nervous system brucellosis by ELISA. . J Neuroimmunol 12:, 173–182. [CrossRef][PubMed]
    [Google Scholar]
  6. Araj G. F., Dhar R., Lastimoza J. L., Haj M.. ( 1990;). Indirect fluorescent antibody test versus enzyme-linked immunosorbent assay and agglutination tests in the serodiagnosis of patients with brucellosis. . Serodiag Immunother Infect Dis 4:, 1–8. [CrossRef]
    [Google Scholar]
  7. Bajani M. D., Ashford D. A., Bragg S. L., Woods C. W., Aye T., Spiegel R. A., Plikaytis B. D., Perkins B. A., Phelan M. et al. & other authors ( 2003;). Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. . J Clin Microbiol 41:, 803–809. [CrossRef][PubMed]
    [Google Scholar]
  8. Brown S. L., Klein G. C., McKinney F. T., Jones W. L.. ( 1981;). Safranin O-stained antigen microagglutination test for detection of brucella antibodies. . J Clin Microbiol 13:, 398–400.[PubMed]
    [Google Scholar]
  9. Buchanan T. M., Faber L. C.. ( 1980;). 2-Mercaptoethanol brucella agglutination test: usefulness for predicting recovery from brucellosis. . J Clin Microbiol 11:, 691–693.[PubMed]
    [Google Scholar]
  10. Cakan G., Bezirci F. B., Kacka A., Cesur S., Aksaray S., Tezeren D., Saka D., Ahmed K.. ( 2008;). Assessment of diagnostic enzyme-linked immunosorbent assay kit and serological markers in human brucellosis. . Jpn J Infect Dis 61:, 366–370.[PubMed]
    [Google Scholar]
  11. CDC ( 1997;). Case definitions for infectious conditions under public health surveillance. . MMWR Recomm Rep 46:, 1–55.[PubMed]
    [Google Scholar]
  12. Fleiss J. L.. ( 1981;). Statistical Methods for Rates and Proportions. New York:: John Wiley & Sons;.
    [Google Scholar]
  13. Gotuzzo E., Carrillo C., Guerra J., Llosa L.. ( 1986;). An evaluation of diagnostic methods for brucellosis – the value of bone marrow culture. . J Infect Dis 153:, 122–125. [CrossRef][PubMed]
    [Google Scholar]
  14. Mantecón M. A., Gutiérrez P., Del Pilar Zarzosa M., Dueñas A. I., Solera J., Fernández-Lago L., Vizcaíno N., Almaraz A., Bratos M. A. et al. & other authors ( 2006;). Utility of an immunocapture-agglutination test and an enzyme-linked immunosorbent assay test against cytosolic proteins from Brucella melitensis B115 in the diagnosis and follow-up of human acute brucellosis. . Diagn Microbiol Infect Dis 55:, 27–35. [CrossRef][PubMed]
    [Google Scholar]
  15. Marrodan T., Nenova-Poliakova R., Rubio M., Ariza J., Clavijo E., Smits H. L., Diaz R.. ( 2001;). Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests. . J Med Microbiol 50:, 663–666.[PubMed]
    [Google Scholar]
  16. Pappas G., Akritidis N., Bosilkovski M., Tsianos E.. ( 2005;). Brucellosis. . N Engl J Med 352:, 2325–2336. [CrossRef][PubMed]
    [Google Scholar]
  17. Sharma R., Chisnall C., Cooke R. P.. ( 2008;). Evaluation of in-house and commercial immunoassays for the sero-diagnosis of brucellosis in a non-endemic low prevalence population. . J Infect 56:, 108–113. [CrossRef][PubMed]
    [Google Scholar]
  18. Sim J., Wright C. C.. ( 2005;). The kappa statistic in reliability studies: use, interpretation, and sample size requirements. . Phys Ther 85:, 257–268.[PubMed]
    [Google Scholar]
  19. Young E. J.. ( 1995;). An overview of human brucellosis. . Clin Infect Dis 21:, 283–289. [CrossRef][PubMed]
    [Google Scholar]
  20. Young E. J., Corbel M. J.. ( 1989;). Brucellosis: Clinical and Laboratory Aspects. Boca Raton, FL:: CRC Press;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.033381-0
Loading
/content/journal/jmm/10.1099/jmm.0.033381-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error