1887

Abstract

In this study of the diversity of AmpC β-lactamase in clinical isolates of spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as using phylogenetic analysis of 16S rRNA and genes. In addition, the species was confirmed by DNA–DNA hybridization. The genetic environment of the gene was characterized, including the and genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated gene originated from the chromosome of .

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2012-01-01
2024-03-28
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