1887

Abstract

In this study of the diversity of AmpC β-lactamase in clinical isolates of spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as using phylogenetic analysis of 16S rRNA and genes. In addition, the species was confirmed by DNA–DNA hybridization. The genetic environment of the gene was characterized, including the and genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated gene originated from the chromosome of .

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2012-01-01
2019-10-16
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References

  1. Bradford P. A. , Urban C. , Mariano N. , Projan S. J. , Rahal J. J. , Bush K. . ( 1997; ). Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the loss of an outer membrane protein. . Antimicrob Agents Chemother 41:, 563–569.[PubMed]
    [Google Scholar]
  2. Brenner D. J. , McWhorter A. C. , Kai A. , Steigerwalt A. G. , Farmer J. J. III . ( 1986; ). Enterobacter asburiae sp. nov., a new species found in clinical specimens, and reassignment of Erwinia dissolvens and Erwinia nimipressuralis to the genus Enterobacter as Enterobacter dissolvens comb. nov. and Enterobacter nimipressuralis comb. nov. . J Clin Microbiol 23:, 1114–1120.[PubMed]
    [Google Scholar]
  3. Chow J. W. , Fine M. J. , Shlaes D. M. , Quinn J. P. , Hooper D. C. , Johnson M. P. , Ramphal R. , Wagener M. M. , Miyashiro D. K. , Yu V. L. . ( 1991; ). Enterobacter bacteremia: clinical features and emergence of antibiotic resistance during therapy. . Ann Intern Med 115:, 585–590.[PubMed] [CrossRef]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. CLSI ( 2010; ). Performance Standards for Antimicrobial Susceptibility Testing, 20th informational supplement M100–S20. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  6. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  7. Hanson N. D. , Sanders C. C. . ( 1999; ). Regulation of inducible AmpC β-lactamase expression among Enterobacteriaceae . . Curr Pharm Des 5:, 881–894.[PubMed]
    [Google Scholar]
  8. Hoffmann H. , Roggenkamp A. . ( 2003; ). Population genetics of the nomenspecies Enterobacter cloacae . . Appl Environ Microbiol 69:, 5306–5318. [CrossRef] [PubMed]
    [Google Scholar]
  9. Jacoby G. A. . ( 2009; ). AmpC β-lactamases. . Clin Microbiol Rev 22:, 161–182. [CrossRef] [PubMed]
    [Google Scholar]
  10. Livermore D. M. , Winstanley T. G. , Shannon K. P. . ( 2001; ). Interpretative reading: recognizing the unusual and inferring resistance mechanisms from resistance phenotypes. . J Antimicrob Chemother 48: (Suppl. 1), 87–102.[PubMed] [CrossRef]
    [Google Scholar]
  11. Paauw A. , Caspers M. P. , Schuren F. H. , Leverstein-van Hall M. A. , Delétoile A. , Montijn R. C. , Verhoef J. , Fluit A. C. . ( 2008; ). Genomic diversity within the Enterobacter cloacae complex. . PLoS ONE 3:, e3018. [CrossRef] [PubMed]
    [Google Scholar]
  12. Pérez-Pérez F. J. , Hanson N. D. . ( 2002; ). Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR. . J Clin Microbiol 40:, 2153–2162. [CrossRef] [PubMed]
    [Google Scholar]
  13. Reisbig M. D. , Hanson N. D. . ( 2002; ). The ACT-1 plasmid-encoded AmpC β-lactamase is inducible: detection in a complex β-lactamase background. . J Antimicrob Chemother 49:, 557–560. [CrossRef] [PubMed]
    [Google Scholar]
  14. Reisbig M. D. , Hossain A. , Hanson N. D. . ( 2003; ). Factors influencing gene expression and resistance for Gram-negative organisms expressing plasmid-encoded ampC genes of Enterobacter origin. . J Antimicrob Chemother 51:, 1141–1151. [CrossRef] [PubMed]
    [Google Scholar]
  15. Rottman M. , Benzerara Y. , Hanau-Berçot B. , Bizet C. , Philippon A. , Arlet G. . ( 2002; ). Chromosomal ampC genes in Enterobacter species other than Enterobacter cloacae, and ancestral association of the ACT-1 plasmid-encoded cephalosporinase to Enterobacter asburiae . . FEMS Microbiol Lett 210:, 87–92. [CrossRef] [PubMed]
    [Google Scholar]
  16. Sanders C. C. , Sanders W. E. Jr , Moland E. S. . ( 1986; ). Characterization of β-lactamases in situ on polyacrylamide gels. . Antimicrob Agents Chemother 30:, 951–952.[PubMed] [CrossRef]
    [Google Scholar]
  17. Schmidt H. , Korfmann G. , Barth H. , Martin H. H. . ( 1995; ). The signal transducer encoded by ampG is essential for induction of chromosomal AmpC β-lactamase in Escherichia coli by β-lactam antibiotics and ‘unspecific’ inducers. . Microbiology 141:, 1085–1092. [CrossRef] [PubMed]
    [Google Scholar]
  18. Song W. , Kim J.-S. , Kim H.-S. , Yong D. , Jeong S. H. , Park M.-J. , Lee K. M. . ( 2006; ). Increasing trend in the prevalence of plasmid-mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosomal ampC gene at a Korean university hospital from 2002 to 2004. . Diagn Microbiol Infect Dis 55:, 219–224. [CrossRef] [PubMed]
    [Google Scholar]
  19. Sørensen A. B. , Duch M. , Jørgensen P. , Pedersen F. S. . ( 1993; ). Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method. . J Virol 67:, 7118–7124.[PubMed]
    [Google Scholar]
  20. Zhou J. , Fries M. R. , Chee-Sanford J. C. , Tiedje J. M. . ( 1995; ). Phylogenetic analyses of a new group of denitrifiers capable of anaerobic growth on toluene and description of Azoarcus tolulyticus sp. nov. . Int J Syst Bacteriol 45:, 500–506. [CrossRef] [PubMed]
    [Google Scholar]
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