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Volume 60, Issue 10

Improved rapid molecular diagnosis of multidrug-resistant tuberculosis using a new reverse hybridization assay, REBA MTB-MDR Free

Abstract

Rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB) is essential for the prompt initiation of effective second-line therapy to improve treatment outcome and limit transmission of this obstinate disease. A variety of molecular methods that enable the rapid detection of mutations implicated in MDR-TB have been developed. The sensitivity of the methods is dependent, in principle, on the repertoire of mutations being detected, which is typically limited to mutations in the genes , and the promoter region of . In this study, a new reverse hybridization assay, REBA MTB-MDR (M&D), that probes mutations in the intergenic region, in addition to those in , and the promoter region, was evaluated. A set of 240 clinical isolates from patients receiving retreatment regimens was subjected to conventional phenotypic drug-susceptibility testing (DST) and the REBA MTB-MDR assay. The nucleotide sequences of the loci known to be involved in drug resistance were determined for comparison. In brief, the results showed that the REBA MTB-MDR assay efficiently recognized nucleotide changes in the intergenic region as well as those in , and the promoter region with higher sensitivity, resulting in an 81.0 % detection rate for isoniazid resistance. Inclusion of the intergenic region in the REBA MTB-MDR assay improved the overall sensitivity of molecular DST for MDR-TB from 73.1 to 79.9 %.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
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2011-10-01
2024-04-18
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Improved rapid molecular diagnosis of multidrug-resistant tuberculosis using a new reverse hybridization assay, REBA MTB-MDR
J. Med. Microbiol. 60, 1447 (2011); https://doi.org/10.1099/jmm.0.032292-0
/content/journal/jmm/10.1099/jmm.0.032292-0
/content/journal/jmm/10.1099/jmm.0.032292-0
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