RT Journal Article SR Electronic(1) A1 Datta, Priya A1 Gulati, Neelam A1 Singla, Nidhi A1 Rani Vasdeva, Hena A1 Bala, Kiran A1 Chander, Jagdish A1 Gupta, VarshaYR 2011 T1 Evaluation of various methods for the detection of meticillin-resistant Staphylococcus aureus strains and susceptibility patterns JF Journal of Medical Microbiology, VO 60 IS 11 SP 1613 OP 1616 DO https://doi.org/10.1099/jmm.0.032219-0 PB Microbiology Society, SN 1473-5644, AB Meticillin-resistant Staphylococcus aureus (MRSA) has been recognized as one of the major pathogens in hospital as well as community settings. In India, the mean isolation rate of MRSA is 20–40 % and many studies have suggested an escalating rate of infections caused by this organism. Despite pharmaceutical and technological advancement, infections caused by MRSA still remain difficult to diagnose. The present study was undertaken to compare five phenotypic methods for the detection of MRSA. This involved examining 200 isolates of S. aureus by oxacillin disc diffusion, cefoxitin disc diffusion, oxacillin screen agar test, the latex agglutination test and growth on CHROMagar. PCR for mecA gene detection was taken as the gold standard. It was found that 35 % of all S. aureus infections were caused by MRSA. The cefoxitin disc diffusion method, as recommended by the Clinical and Laboratory Standards Institute, was found to be a reliable method for MRSA detection but it should be supplemented with some other method like latex agglutination, CHROMagar or oxacillin screen agar testing so that no MRSA is missed. We recommend that along with cefoxitin disc diffusion, another method, preferably latex agglutination, should be routinely used in all hospitals to detect MRSA., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.032219-0