1887

Abstract

The pathogenesis of the primary stage of lymphogranuloma venereum (LGV) is poorly understood. There is no skin cell model and LGV pathogenesis studies are therefore carried out on cells of different origin. Moreover, such studies usually use reference strains, which may have evolved over the years in culture. In this study, a model was developed in which enters and grows in human keratinocytes at 37 and 33 °C. Keratinocytes were infected with fresh clinical isolates and the three LGV reference strains L1, L2 and L3. Growth was monitored for 5 days post-infection using fluorescence microscopy and image analysis software. Chlamydial replication was quicker at 37 than at 33 °C, despite 33 °C being the temperature of human skin. The serovar L2 reference strain grew significantly faster than the other strains, although the fresh clinical isolates were also serovar L2. When grown in keratinocytes at 33 °C, the L2 and L3 reference strains produced much larger inclusions than the other strains tested. This model, which utilizes keratinocytes, better simulates the conditions present at the initial site of infection in LGV than previously published literature, making it a useful tool for future LGV pathogenesis studies. In addition, the results indicate that fresh clinical isolates should be included in LGV pathogenesis studies.

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2011-11-01
2024-03-28
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References

  1. Abou Elela S., Nazar R. N. 1997; Role of the 5.8S rRNA in ribosome translocation. Nucleic Acids Res 25:1788–1794 [CrossRef]
    [Google Scholar]
  2. Black C. M. 1997; Current methods of laboratory diagnosis of Chlamydia trachomatis infections. Clin Microbiol Rev 10:160–184[PubMed]
    [Google Scholar]
  3. Boukamp P., Petrussevska R. T., Breitkreutz D., Hornung J., Markham A., Fusenig N. E. 1988; Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 106:761–771 [View Article][PubMed]
    [Google Scholar]
  4. Carlson J. H., Porcella S. F., McClarty G., Caldwell H. D. 2005; Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains. Infect Immun 73:6407–6418 [View Article][PubMed]
    [Google Scholar]
  5. Fields K. A., Fischer E., Hackstadt T. 2002; Inhibition of fusion of Chlamydia trachomatis inclusions at 32 °C correlates with restricted export of IncA. Infect Immun 70:3816–3823 [View Article][PubMed]
    [Google Scholar]
  6. Maleka D. M., Hoosen A. A., Sturm A. W., Kiepiela P. 1996; The laboratory diagnosis of lymphogranuloma venereum (LGV) . MMedSc dissertation, University of Natal Medical School, Department of Medical Microbiology; Durban, South Africa:
    [Google Scholar]
  7. Miyairi I., Mahdi O. S., Ouellette S. P., Belland R. J., Byrne G. I. 2006; Different growth rates of Chlamydia trachomatis biovars reflect pathotype. J Infect Dis 194:350–357 [View Article][PubMed]
    [Google Scholar]
  8. Moodley P., Sturm P. D. J., Vanmali T., Wilkinson D., Connolly C., Sturm A. W. 2003; Association between HIV-1 infection, the etiology of genital ulcer disease, and response to syndromic management. Sex Transm Dis 30:241–245 [View Article][PubMed]
    [Google Scholar]
  9. O’Farrell N., Morison L., Moodley P., Pillay K., Vanmali T., Quigley M., Sturm A. W. 2008; Genital ulcers and concomitant complaints in men attending a sexually transmitted infections clinic: implications for sexually transmitted infections management. Sex Transm Dis 35:545–549 [View Article][PubMed]
    [Google Scholar]
  10. Schachter J., Osoba A. O. 1983; Lymphogranuloma venereum. Br Med Bull 39:151–154[PubMed]
    [Google Scholar]
  11. Schachter J., Smith D. E., Dawson C. R., Anderson W. R., Deller J. J. Jr, Hoke A. W., Smartt W. H., Meyer K. F. 1969; Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J Infect Dis 120:372–375 [View Article][PubMed]
    [Google Scholar]
  12. Sturm P. D. J., Moodley P., Govender K., Bohlken L., Vanmali T., Sturm A. W. 2005; Molecular diagnosis of lymphogranuloma venereum in patients with genital ulcer disease. J Clin Microbiol 43:2973–2975 [View Article][PubMed]
    [Google Scholar]
  13. Van Ooij C., Homola E., Kincaid E., Engel J. 1998; Fusion of Chlamydia trachomatis-containing inclusions is inhibited at low temperatures and requires bacterial protein synthesis. Infect Immun 66:5364–5371[PubMed]
    [Google Scholar]
  14. Ward M. E. 2002; Chlamydia. In Medical Microbiology, 16th edn. pp. 358–368 Edited by Greenwood D., Slack R. C. B., Peutherer J. F. London: Elsevier Science;
    [Google Scholar]
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