1887

Abstract

Prairie dogs () are used to study the aetiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. Epidemiological and experimental studies have suggested a connection between infection with species and cholesterol cholelithiasis, cholecystis and gallbladder cancer. Ten of the 34 prairie dogs in this study had positive species identified by PCR using genus-specific primers. Ten of 34 prairie dogs had positive species identified in the intestine by PCR with genus-specific primers. Six sp. isolates and three sp. isolates were identified taxonomically by 16S rRNA gene analysis. The prairie dog helicobacters fell into three clusters adjacent to . On the basis of 16S rRNA gene sequence analysis, three strains in two adjacent clusters were included in the species . Three strains were only 97.1 % similar to the sequence of and can be considered a novel species with the provisional designation sp. Prairie Dog 3. The prairie dog campylobacters formed a single novel cluster and represent a novel sp. with the provisional designation sp. Prairie Dog. They branched with at 96.3 % similarity and had the greatest sequence similarity to at 97.1 % similarity. Whether or the novel sp. and sp. identified in prairie dogs play a role in cholesterol gallstones or hepatobiliary disease requires further studies.

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2011-09-01
2019-10-19
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