1887

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Volume 60, Issue 9

Molecular epidemiology of group B streptococcal meningitis in children beyond the neonatal period from Angola Free

Abstract

is a major pathogen of neonates and immunocompromised adults. Prior studies have demonstrated that, beyond the neonatal period, rarely causes invasive infections in children. However, during 2004–2005, was the causative agent of 60 meningitis episodes in children aged 3 months to 12 years from Angola. To identify and study the specific causative genetic lineages of childhood meningitis, which lack characterization to date, we conducted an extensive molecular analysis of the recovered isolates ( = 21). This constitutes what we believe to be the first molecular study of the population structure of invasive isolates from Africa. A low genetic diversity was observed among the isolates, where the majority belonged to clonal complex (CC) 17 presenting the capsular subtype III-2 (86 % of cases) and marked by the intron group II GBSi1, which has previously been observed to be associated with neonatal hosts. The predominance of single-locus variants of sequence type (ST) 17 suggested the local diversification of this hypervirulent clone, which displayed novel alleles of the and virulence genes. The absence of the region in two isolates with the Ia/ST23 genotype is more typical of cattle than human isolates. Globally, these data provide novel information about the enhanced invasiveness of the CC17 genetic lineage in older children and suggest the local diversification of this clone, which may be related to the future emergence of a novel epidemic clone in Angola.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
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2011-09-01
2024-04-19
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References

  1. Al Safadi R., Amor S., Hery-Arnaud G., Spellerberg B., Lanotte P., Mereghetti L., Gannier F., Quentin R., Rosenau A. 2010; Enhanced expression of lmb gene encoding laminin-binding protein in Streptococcus agalactiae strains harboring IS1548 in scpB-lmb intergenic region. PLoS ONE 5:e10794 [View Article][PubMed]
     [Google Scholar]
  2. Bernardino L., Magalhães J., Simões M. J., Monteiro L. 2003; Bacterial meningitis in Angola. Lancet 361:1564–1565 [View Article][PubMed]
     [Google Scholar]
  3. Bohnsack J. F., Whiting A., Gottschalk M., Dunn D. M., Weiss R., Azimi P. H., Philips J. B. III, Weisman L. E., Rhoads G. G., Lin F. Y. 2008; Population structure of invasive and colonizing strains of Streptococcus agalactiae from neonates of six US Academic Centers from 1995 to 1999. J Clin Microbiol 46:1285–1291 [View Article][PubMed]
     [Google Scholar]
  4. Brochet M., Couvé E., Zouine M., Vallaeys T., Rusniok C., Lamy M. C., Buchrieser C., Trieu-Cuot P., Kunst F., Poyart C. 2006; Genomic diversity and evolution within the species Streptococcus agalactiae . Microbes Infect 8:1227–1243 [View Article][PubMed]
     [Google Scholar]
  5. Brochet M., Couvé E., Bercion R., Sire J. M., Glaser P. 2009; Population structure of human isolates of Streptococcus agalactiae from Dakar and Bangui. J Clin Microbiol 47:800–803 [View Article][PubMed]
     [Google Scholar]
  6. CLSI 2009; Performance Standards for Antimicrobial Susceptibility Testing; 19th Informational Supplement. M100-S19. Wayne, PA: Clinical and Laboratory Standards Institute;
  7. de Azavedo J. C., McGavin M., Duncan C., Low D. E., McGeer A. 2001; Prevalence and mechanisms of macrolide resistance in invasive and noninvasive group B Streptococcus isolates from Ontario, Canada. Antimicrob Agents Chemother 45:3504–3508 [View Article][PubMed]
     [Google Scholar]
  8. Florindo C., Viegas S., Paulino A., Rodrigues E., Gomes J. P., Borrego M. J. 2010; Molecular characterization and antimicrobial susceptibility profiles in Streptococcus agalactiae colonizing strains: association of erythromycin resistance with subtype III-1 genetic clone family. Clin Microbiol Infect 16:1458–1463 [View Article][PubMed]
     [Google Scholar]
  9. Franken C., Haase G., Brandt C., Weber-Heynemann J., Martin S., Lämmler C., Podbielski A., Lütticken R., Spellerberg B. 2001; Horizontal gene transfer and host specificity of β-haemolytic streptococci: the role of a putative composite transposon containing scpB and lmb . Mol Microbiol 41:925–935 [View Article][PubMed]
     [Google Scholar]
  10. Gagneur A., Héry-Arnaud G., Croly-Labourdette S., Gremmo-Feger G., Vallet S., Sizun J., Quentin R., Tandé D. 2009; Infected breast milk associated with late-onset and recurrent group B streptococcal infection in neonatal twins: a genetic analysis. Eur J Pediatr 168:1155–1158 [View Article][PubMed]
     [Google Scholar]
  11. Gherardi G., Imperi M., Baldassarri L., Pataracchia M., Alfarone G., Recchia S., Orefici G., Dicuonzo G., Creti R. 2007; Molecular epidemiology and distribution of serotypes, surface proteins, and antibiotic resistance among group B streptococci in Italy. J Clin Microbiol 45:2909–2916 [View Article][PubMed]
     [Google Scholar]
  12. Gray K. J., Bennett S. L., French N., Phiri A. J., Graham S. M. 2007; Invasive group B streptococcal infection in infants, Malawi. Emerg Infect Dis 13:223–229 [View Article][PubMed]
     [Google Scholar]
  13. Gygax S. E., Schuyler J. A., Kimmel L. E., Trama J. P., Mordechai E., Adelson M. E. 2006; Erythromycin and clindamycin resistance in group B streptococcal clinical isolates. Antimicrob Agents Chemother 50:1875–1877 [View Article][PubMed]
     [Google Scholar]
  14. Jones N., Bohnsack J. F., Takahashi S., Oliver K. A., Chan M.-S., Kunst F., Glaser P., Rusniok C., Crook D. W. et al. 2003; Multilocus sequence typing system for group B Streptococcus. J Clin Microbiol 41:2530–2536 [View Article][PubMed]
     [Google Scholar]
  15. Jones N., Oliver K. A., Barry J., Harding R. M., Bisharat N., Spratt B. G., Peto T., Crook D. W. Oxford Group B Streptococcus Consortium 2006; Enhanced invasiveness of bovine-derived neonatal sequence type 17 group B Streptococcus is independent of capsular serotype. Clin Infect Dis 42:915–924 [View Article][PubMed]
     [Google Scholar]
  16. Kim K. S. 2010; Acute bacterial meningitis in infants and children. Lancet Infect Dis 10:32–42 [View Article][PubMed]
     [Google Scholar]
  17. Lin F.-Y., Weisman L. E., Troendle J., Adams K. 2003; Prematurity is the major risk factor for late-onset group B streptococcus disease. J Infect Dis 188:267–271 [View Article][PubMed]
     [Google Scholar]
  18. Liu G. Y.-H., Nizet V. 2004; Extracellular virulence factors of group B streptococci. Front Biosci 9:1794–1802 [View Article][PubMed]
     [Google Scholar]
  19. Luan S.-L., Granlund M., Sellin M., Lagergård T., Spratt B. G., Norgren M. 2005; Multilocus sequence typing of Swedish invasive group B Streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching. J Clin Microbiol 43:3727–3733 [View Article][PubMed]
     [Google Scholar]
  20. Madhi S. A., Radebe K., Crewe-Brown H., Frasch C. E., Arakere G., Mokhachane M., Kimura A. 2003; High burden of invasive Streptococcus agalactiae disease in South African infants. Ann Trop Paediatr 23:15–23 [View Article][PubMed]
     [Google Scholar]
  21. Manning S. D., Springman A. C., Lehotzky E., Lewis M. A., Whittam T. S., Davies H. D. 2009; Multilocus sequence types associated with neonatal group B streptococcal sepsis and meningitis in Canada. J Clin Microbiol 47:1143–1148 [View Article][PubMed]
     [Google Scholar]
  22. Manning S. D., Springman A. C., Million A. D., Milton N. R., McNamara S. E., Somsel P. A., Bartlett P., Davies H. D. 2010; Association of group B Streptococcus colonization and bovine exposure: a prospective cross-sectional cohort study. PLoS ONE 5:e8795 [View Article][PubMed]
     [Google Scholar]
  23. Mullaney D. M. 2001; Group B streptococcal infections in newborns. J Obstet Gynecol Neonatal Nurs 30:649–658 [View Article][PubMed]
     [Google Scholar]
  24. Pelkonen T., Roine I., Monteiro L., Correia M., Pitkäranta A., Bernardino L., Peltola H. 2009; Risk factors for death and severe neurological sequelae in childhood bacterial meningitis in sub-Saharan Africa. Clin Infect Dis 48:1107–1110 [View Article][PubMed]
     [Google Scholar]
  25. Rato M. G., Bexiga R., Nunes S. F., Cavaco L. M., Vilela C. L., Santos-Sanches I. 2008; Molecular epidemiology and population structure of bovine Streptococcus uberis . J Dairy Sci 91:4542–4551 [View Article][PubMed]
     [Google Scholar]
  26. Rolland K., Marois C., Siquier V., Cattier B., Quentin R. 1999; Genetic features of Streptococcus agalactiae strains causing severe neonatal infections, as revealed by pulsed-field gel electrophoresis and hylB gene analysis. J Clin Microbiol 37:1892–1898[PubMed]
     [Google Scholar]
  27. Sáez-Llorens X., McCracken G. H. Jr 2003; Bacterial meningitis in children. Lancet 361:2139–2148 [View Article][PubMed]
     [Google Scholar]
  28. Schrag S. J., Zywicki S., Farley M. M., Reingold A. L., Harrison L. H., Lefkowitz L. B., Hadler J. L., Danila R., Cieslak P. R., Schuchat A. 2000; Group B streptococcal disease in the era of intrapartum antibiotic prophylaxis. N Engl J Med 342:15–20 [View Article][PubMed]
     [Google Scholar]
  29. Slotved H. C., Kong F., Lambertsen L., Sauer S., Gilbert G. L. 2007; Serotype IX, a proposed new Streptococcus agalactiae serotype. J Clin Microbiol 45:2929–2936 [View Article][PubMed]
     [Google Scholar]
  30. Sørensen U. B., Poulsen K., Ghezzo C., Margarit I., Kilian M. 2010; Emergence and global dissemination of host-specific Streptococcus agalactiae clones. mBio 1:e00178-10 [View Article][PubMed]
     [Google Scholar]
  31. Springman A. C., Lacher D. W., Wu G., Milton N., Whittam T. S., Davies H. D., Manning S. D. 2009; Selection, recombination, and virulence gene diversity among group B streptococcal genotypes. J Bacteriol 191:5419–5427 [View Article][PubMed]
     [Google Scholar]
  32. Sutcliffe J., Tait-Kamradt A., Wondrack L. 1996; Streptococcus pneumoniae and Streptococcus pyogenes resistant to macrolides but sensitive to clindamycin: a common resistance pattern mediated by an efflux system. Antimicrob Agents Chemother 40:1817–1824[PubMed]
     [Google Scholar]
  33. Tazi A., Disson O., Bellais S., Bouaboud A., Dmytruk N., Dramsi S., Mistou M. Y., Khun H., Mechler C. et al. 2010; The surface protein HvgA mediates group B streptococcus hypervirulence and meningeal tropism in neonates. J Exp Med 207:2313–2322 [View Article][PubMed]
     [Google Scholar]
  34. Trager J. D., Martin J. M., Barbadora K., Green M., Wald E. R. 1996; Probable community acquisition of group B Streptococcus in an infant with late-onset disease: demonstration using field inversion gel electrophoresis. Arch Pediatr Adolesc Med 150:766–768[PubMed] [CrossRef]
     [Google Scholar]
  35. Tzanakaki G., Mastrantonio P. 2007; Aetiology of bacterial meningitis and resistance to antibiotics of causative pathogens in Europe and in the Mediterranean region. Int J Antimicrob Agents 29:621–629 [View Article][PubMed]
     [Google Scholar]
  36. WHO 2010; World Health Statistics 2010. Geneva: World Health Organization; http://www.who.int/whosis/whostat/2010/en/
  37. Zhao Z., Kong F., Zeng X., Gidding H. F., Morgan J., Gilbert G. L. 2008; Distribution of genotypes and antibiotic resistance genes among invasive Streptococcus agalactiae (group B streptococcus) isolates from Australasian patients belonging to different age groups. Clin Microbiol Infect 14:260–267 [View Article][PubMed]
     [Google Scholar]
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Molecular epidemiology of group B streptococcal meningitis in children beyond the neonatal period from Angola
J. Med. Microbiol. 60, 1276 (2011); https://doi.org/10.1099/jmm.0.031674-0
/content/journal/jmm/10.1099/jmm.0.031674-0
/content/journal/jmm/10.1099/jmm.0.031674-0
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