1887

Abstract

A total of 817 human clinical isolates of from all Australian states were screened for AB strains by toxin gene PCR assays. Nine (1.1 %) strains were confirmed to be AB by enzyme immunoassay for toxin production. Of these, six (66.7 %) were binary toxin-positive by PCR. Using PCR ribotyping and toxinotyping, the AB strains could be grouped into seven ribotypes and three toxinotypes. Only one of the ribotypes had been reported previously (017). The prevalence of ribotype 017 was low in this study with only two strains detected. Two new AB toxinotypes were also defined (XXX, XXXI). Toxinotype XXX had a toxin B gene similar to that of toxinotype IV (AB) but with a novel cytopathic region. Toxinotype XXXI was similar to other AB types (X, XVII), but had a larger deletion to the toxin A gene than in either of those types. The types of AB strains identified in this study differed markedly from those described in other regions.

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2011-08-01
2019-09-23
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