1887

Abstract

strains of toxinotypes III ( = 13) and V ( = 45) were typed by agarose gel-based PCR ribotyping, capillary gel electrophoresis-based PCR ribotyping and PFGE using two different restriction enzymes, I and II. With conventional agarose gel-based PCR ribotyping, toxinotype III strains were distributed among six different PCR ribotypes and toxinotype V strains into three different PCR ribotypes. Capillary gel electrophoresis-based ribotyping was more discriminatory for toxinotype V strains, with six different ribotypes found. With PFGE using I, all toxinotype III strains grouped together into a single pulsotype. Using II, ribotype 027 strains grouped together with >90 % similarity and were <83 % similar to other ribotypes of toxinotype III strains. Within ribotype 078, seven (aI) and eight (cII) different pulsotypes were found, whilst ribotype 126 strains belonged to one (I) and two (II) pulsotypes. Within ribotype 066, it was possible to distinguish between pig and human isolates. Using II, a further distinction could also be made between pig isolates from two different farms. PFGE (I and II) clustered strains according to their toxinotype; however, correlation of PFGE and ribotyping was better with II. These data suggest that toxinotype III strains are a more heterogeneous group than toxinotype V strains and that II is more discriminatory than I. Alternatively, the use of both enzymes simultaneously could improve PFGE typing of .

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2011-08-01
2019-12-13
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