1887

Abstract

Invasive aspergillosis is associated with high morbidity and mortality rates; nevertheless, blood cultures almost invariably yield a negative result. The recovery and detection time of , and were studied in BACTEC Plus Aerobic/F, Mycosis-IC/F and Myco/F Lytic vials, incubated in the BACTEC 9240 and 9000 MB automated systems. Two different approaches were used for subculture in solid medium: (i) the routine method, using a sterile airway needle/subculture unit, and (ii) a novel procedure, using instead a tuberculin disposable syringe and collecting a larger aliquot (100 µl), following vigorous agitation of the vials. was detected at inoculum concentrations of >3 conidia per 10 ml after 21–40 h, in both BACTEC Plus Aerobic/F and BACTEC Mycosis-IC/F vials. A few more hours were needed to detect and . The novel subculture procedure of BACTEC culture vials on solid medium resulted in several positive results that were not detected by the routine sampling procedure. BACTEC Plus Aerobic/F vials show an advantage particularly in patients under antifungal treatment. In cases of polymicrobial bloodstream infections (concurrent bacterial growth), the inoculation of blood samples into a BACTEC Mycosis-IC/F vial achieved the best results. Further multicentre studies are needed to validate this improved automated detection of spp. from blood cultures in clinical laboratories, as this diagnostic procedure allows antifungal susceptibility testing of moulds.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.030601-0
2011-10-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/10/1467.html?itemId=/content/journal/jmm/10.1099/jmm.0.030601-0&mimeType=html&fmt=ahah

References

  1. Araujo R. , Rodrigues A. G. . ( 2004; ). Variability of germinative potential among pathogenic species of Aspergillus . . J Clin Microbiol 42:, 4335–4337. [CrossRef] [PubMed]
    [Google Scholar]
  2. Araujo R. , Rodrigues A. G. , Pina-Vaz C. . ( 2004; ). A fast, practical and reproducible procedure for the standardization of the cell density of an Aspergillus suspension. . J Med Microbiol 53:, 783–786. [CrossRef] [PubMed]
    [Google Scholar]
  3. Araujo R. , Pina-Vaz C. , Rodrigues A. G. , Amorim A. , Gusmão L. . ( 2009; ). Simple and highly discriminatory microsatellite-based multiplex PCR for Aspergillus fumigatus strain typing. . Clin Microbiol Infect 15:, 260–266. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bajjoka I. E. , Bailey E. M. , Vazquez J. A. , Abouljoud M. S. . ( 1999; ). Combination antifungal therapy for invasive aspergillosis infection in liver transplant recipients: report of two patients. . Pharmacotherapy 19:, 118–123. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bouza E. , Sousa D. , Rodríguez-Créixems M. , Lechuz J. G. , Muñoz P. . ( 2007; ). Is the volume of blood cultured still a significant factor in the diagnosis of bloodstream infections?. J Clin Microbiol 45:, 2765–2769.[CrossRef]
    [Google Scholar]
  6. Dagenais T. R. , Keller N. P. . ( 2009; ). Pathogenesis of Aspergillus fumigatus in invasive aspergillosis. . Clin Microbiol Rev 22:, 447–465. [CrossRef] [PubMed]
    [Google Scholar]
  7. Del Bono V. , Mikulska M. , Viscoli C. . ( 2008; ). Invasive aspergillosis: diagnosis, prophylaxis and treatment. . Curr Opin Hematol 15:, 586–593. [CrossRef] [PubMed]
    [Google Scholar]
  8. De Pauw B. , Walsh T. J. , Donnelly J. P. , Stevens D. A. , Edwards J. E. , Calandra T. , Pappas P. G. , Maertens J. , Lortholary O. et al. ( 2008; ). Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. . Clin Infect Dis 46:, 1813–1821. [CrossRef] [PubMed]
    [Google Scholar]
  9. Freifeld A. , Arnold S. , Ooi W. , Chen F. , Meyer T. , Wheat L. J. , Smedema M. , Lemonte A. , Connolly P. . ( 2007; ). Relationship of blood level and susceptibility in voriconazole treatment of histoplasmosis. . Antimicrob Agents Chemother 51:, 2656–2657. [CrossRef] [PubMed]
    [Google Scholar]
  10. Fuller D. D. , Davis T. E. Jr , Denys G. A. , York M. K. . ( 2001; ). Evaluation of BACTEC MYCO/F Lytic medium for recovery of mycobacteria, fungi, and bacteria from blood. . J Clin Microbiol 39:, 2933–2936. [CrossRef] [PubMed]
    [Google Scholar]
  11. Girmenia C. , Nucci M. , Martino P. . ( 2001; ). Clinical significance of Aspergillus fungaemia in patients with haematological malignancies and invasive aspergillosis. . Br J Haematol 114:, 93–98. [CrossRef] [PubMed]
    [Google Scholar]
  12. Meyer M. H. , Letscher-Bru V. , Jaulhac B. , Waller J. , Candolfi E. . ( 2004; ). Comparison of Mycosis IC/F and Plus Aerobic/F media for diagnosis of fungemia by the Bactec 9240 system. . J Clin Microbiol 42:, 773–777. [CrossRef] [PubMed]
    [Google Scholar]
  13. Nucci M. , Anaissie E. . ( 2007; ). Fusarium infections in immunocompromised patients. . Clin Microbiol Rev 20:, 695–704. [CrossRef] [PubMed]
    [Google Scholar]
  14. Perlin D. S. , Zhao Y. . ( 2009; ). Molecular diagnostic platforms for detecting Aspergillus . . Med Mycol 47: (Suppl. 1), S223–S232.[PubMed] [CrossRef]
    [Google Scholar]
  15. Sande W. V. , Tavakol M. , Vianen W. V. , Bakker-Woudenberg I. A. . ( 2009; ). The effects of antifungal agents to conidial and hyphal forms of Aspergillus fumigatus . . Med Mycol 18:, 1–8. [CrossRef] [PubMed]
    [Google Scholar]
  16. Segal B. H. . ( 2009; ). Aspergillosis. . N Engl J Med 360:, 1870–1884. [CrossRef] [PubMed]
    [Google Scholar]
  17. Thuret G. , Carricajo A. , Vautrin A. C. , Raberin H. , Acquart S. , Garraud O. , Gain P. , Aubert G. . ( 2005; ). Efficiency of blood culture bottles for the fungal sterility testing of corneal organ culture media. . Br J Ophthalmol 89:, 586–590. [CrossRef] [PubMed]
    [Google Scholar]
  18. Vetter E. , Torgerson C. , Feuker A. , Hughes J. , Harmsen S. , Schleck C. , Horstmeier C. , Roberts G. , Cockerill F. III . ( 2001; ). Comparison of the BACTEC MYCO/F Lytic bottle to the isolator tube, BACTEC Plus Aerobic F/bottle, and BACTEC Anaerobic Lytic/10 bottle and comparison of the BACTEC Plus Aerobic F/bottle to the Isolator tube for recovery of bacteria, mycobacteria, and fungi from blood. . J Clin Microbiol 39:, 4380–4386. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.030601-0
Loading
/content/journal/jmm/10.1099/jmm.0.030601-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error